Shared Flashcard Set

Details

Microbiology Exam
fdfsdf
354
Biology
Undergraduate 2
05/09/2012

Additional Biology Flashcards

 


 

Cards

Term
What are the characteristics of a Gram + beta-lactamase?
Definition
1) Similar substrate specificity
2) Active against penicillin
3) Inducible
4) Produced in large quantities
5) excreted into the external environment
6)Plasmid oriented
Term
What are the characteristics of a Gram - beta-lactamase?
Definition
1) Different substrate specificity (spectrum)
2)Active against penicillin and cephalosporins
3) Consititutive or Inducible
4) Produced in small quantities
5) Excreted into Periplasmic space
6) Plasmid/Chromosomal Oriented
Term
Which is better to have a low inducer or high inducer when considered beta-lactamases?
Definition
High inducer for the inducible beta-lactamase will be seen right away and can be taken care of.

Low inducers have the patient originally get better then crash as the beta lactamase is produced.
Term
What are 3 sensitivity tests?
Definition
1) Disk Diffusion- Kirby Bauer (place many disks on agar and look for zones of inhibition)
2) MIC (Minimum Inhibitory Concentration) Determination
3) E-test (combo of MIC and Disk)
Term
What are the 6 limitations of Sensitivity Tests?
Definition
1) Site of infection-abscess or foreign body
2) Host infections- protein binding and immune response
3) Pharmokinetics- metabolism and elimination for antibiotic concentration decreases over time
4) Post antibiotic effect
5) Sensitivity end points- test every 24hrs dose patient ever 6hrs
6) Inoculum- how much bacteria is present
Term
What are the 6 Methods of Antibiotics?
What antibiotics fit under each?
Definition
1) Folate Metabolism- sulfanomides
2) Cell Wall- penicillins, cephalosporins, vancomysin, monobactam
3) Protein Synthesis- tetracycline, aminoglycosides, erthromycin, chlorophenicol
4) Cell membrane- polymyxin B amphoterium B(very toxic)
5) DNA Replication (DNA gyrase)- quinolones
6) RNA Polymerase- rifampin
Term
What are 7 types of antibiotic resistance?
Definition
1) Altered transpeptidase
2) Altered RNA polymerase
3) Altered DNA Gryase
4) increased efflux and decrased uptake
5) Altered porin structure
6) beta-lactamase
7) cephalosporinase
Term
What are the four stages of Bacterial Growth?
Where are antibiotics working the most?
Definition
1) Lag Phase- no growth
2) Exponential phase- rapid growth (bacteria start to release toxin and antibiotics are most active)
3) Stationary- cell division = cell death due to toxins
4) Death- eventually all bacteria die to toxin
Term
What are the 3 major types (with subcategories) of types of Bacterial growth? ie oxygen etc
Definition
1) Aerobes- need 02
a)Strict aerobes- any 02 kills them
b) microaerophillic- grow with lil 02, but love c02
2) anaerobes- die in 02
a)Strict anaerobes- any 02 kills them
b)Aerotolerant- can survive in a lil 02
3) Facultative- can grow both aerobically and anaerobically (most pathogens)
Term
What are the 4 Steps to DNA replication?
Definition
1) Initiation
2) Elongation
3) Proofread Product
4) Termination
Term
What are the 3 ways that Bacteria transfer DNA?
Which is the most common?
Definition
1) Transformation- bacteria dies/lysis. DNA fragments are taken up by another
2)Transduction- virus infects bacteria and picks up some bacterial DNA. When it infects another bacteria it transfers the DNA to it
3) Conjugation- bacterial sex pili used to transfer whole chromosome or plasmids (most common, see transposons-jumping genes)
Term
Describe a Gram + cell Wall
Definition
1) Structural proteins on outtermost part
2) Thick peptidoglycan
3)Cytoplasmic membrane that contains transport proteins
4) Not very complex
Term
Describe a Gram - Cell Wall
Definition
1) Lipopolysaccharide (LPS)
a)O antigen
b)Core
c)Lipid A
2) Thin peptidoglycan layer
3)Periplasmic space- holds antibiotics to develop resistance
4) Cell membrane containing porins
Term
Describe the Steps to a Gram Stain
Definition
1) Heat fix bacteria to slide
2) Flood with crystal violet which dyes all bacteria blue, then wash off
3) Flood with Grams Iodine which forms complex molecule in bacteria, wash off
4) Decolorize with alcohol or acetone- breaks up molecule in gram -
5) Add saffrennin- dyes gram - red
Term
What is the Gram stain of Group A Strep?
Definition
Gram + cocci in chains
Term
How is Group A Strep transfered?
Definition
person to person
Term
What are the virulence factors of Group A Strep?
Definition
1) Capsule
2) Hyaluronidase-eats tissue to cause necrotic
3) Streptolysin O/S- betahemolytic
4) Erthyrogenic toxin- rash
5) Streptokinase- dissolves clots
6) Streptodornase- dissolves clots
3)
Term
What are the 10 diseases caused by Group A Strep?
Definition
1) Pharyngitis-most common bacterial cause
2) Fulminate pneumonia
3) Pyoderma- red hot skin
4) Erysipelas- skin peels off
5) impetigo- boils
6) Necrotizing Faciitis- flesh eating bacteria
7) Toxic Shock Syndrome
8) Scarlet Fever- rash
9) Rheumatic Fever- endocarditis due to heart valve damage
10) Acute glomerulonephritis
Term
How is Group A Strep diagnosed?
Definition
1) Throat Culture
2) A Disk (Basitracin) on blood agar
Term
How is Group A Strep treated?
Definition
1) Penicillin up to 9 days after diagnosis
2) Amputation/filet of necrotized tissue
Term
What is the gram and shape of Group B Strep?
Definition
Gram + cocci in chains
Term
What are the Virulence Factors for Group B Strep?
Definition
1) Capsule
2) Hyaluronidase
Term
Which types of Strep are betahemolytic?
Which ones are alphahemoltyic?
Definition
Betahemolytic
1)Group A
2)Group B
3)Group C,G,F
Alpha-hemolytic
1)Strep. pneumonia
2)Viridans Srep
Term
How is Group B Strep transmitted?
Definition
Vertical Transmission (Birth canal)
Treat women at 38weeks to prevent transfer to baby
Term
What 5 diseases are caused by Group B Strep?
Definition
1) Neonatal sepsis/meningitis
2) Postpartum sepsis/meningitis
3) Sepsis
4) Osteomyelitis
5) Wound Infections
Term
How is Group B Strep Treated?
Definition
Penicillin
Term
How is Group B Strep Diagnosed?
Definition
Camp test
Term
What is the gram and shape of Group C,G,F Strep?
Definition
Gram + cocci in chains
Term
What 6 diseases are caused by Group C,G,F Strep
Definition
1)Cellulitis
2)Endocarditis
3)Sinusitis
4)Abscess
5)Bacteremia
6)Pharyngitis
Term
How is Group C,G,F Strep treated?
Definition
1) Amoxicillin
2) Penicillin
Term
What is the gram stain and shape of Strep. pneumonia?
Definition
Gram + diplococci
Term
What are the virulence factors of strep. pneumonia?
Definition
1) Capsule
2) Hyaluronidase
Term
How is Strep pneumonia transmitted?
Definition
person to person
Term
What are the 7 diseases caused by strep. pneumonia?
Definition
1)Lobar Pneumonia
2)Meningitis
3)Sinusitis
4)Otitis Media
5)Mastoiditis
6)PErtonitis
7)Bacteremia
Term
What two groups are at risk for strep. pneumonia?
Definition
Blacks and elderly
Term
What disease parallels strep. pneumonia?
Definition
flu
Term
How does the vaccine for strep. pneumonia work? Who is it for?
Definition
1)develops antibodies against the polysaccharides but doesnt cover all (19A)
2)for those over 60
Term
How is Strep pneumonia diagnosed?
Definition
P Disk(Optochin) on blood agar
Term
How is Strep pneumonia treated?
Definition
Penicillin
Term
What is the gram and shape of Viridans Strep?
Definition
Gram + cocci in pairs
Term
What are the virulence factors of Viridans Strep?
Definition
1) Capsule
2) Hyaluronidase
Term
What 3 diseases does Viridans Strep cause?
Definition
1)Subacute Bacterial Endocarditis
2) Dental Carries
3) Abscess
Term
What is one specific type of Viridans Strep? What does it indicate?
Definition
Strep bovis indicates GI Cancer
Term
What is the treatment for Viridans Strep?
Definition
1) Aminoglycosides
2) Penicillin
Term
What two specific bacteria indicate GI cancer?
Definition
1) Strep bovis
2) Clostridia septicum
Term
What is the gram and shape of Genus Enterococcus?
Definition
gram + cocci
Term
What 5 diseases are caused by Genus enterococcus?
Definition
1) Vancomysin resistant enterococcus (VRE)
2) UTI
3) Wound Infections
4) Abscesses
5) Bacteremia
Term
How is genus enterococcus treated?
Definition
Vancomysin
Term
What is the definition of an enteric?
Definition
gram - rod that is part of the normal bowel flora
Term
What are the 4 bacteria that can cause meningitis?
Definition
1) Group B Strep
2) Strep. pneumonia
3) Nisseria Meningiditis
4) Haemophilus influenzae
Term
What are the two bacteria that can grow on a Jembec plate?
Definition
1) Nisseria Gonorrhea
2) Moraxella Catarrhalis
Term
What is the gram of Staph. Aureus?
Definition
Gram + cocci in clusters
Term
What are the two types of MRSA and how are they transmitted?
Definition
1) Hospital acquired- hands to hands
2) Community Acquired- skin to skin
Term
What are the 7 virulence Factors of Staph. Aureus?
Definition
1) Enterotoxin (diarrhea, gastroenteritis, food poisoning)
2) Cytolytic (betahemolytic)
3) Toxic Shock syndrome Toxin
4) Exfoliative Toxin (scalded skin syndrome)
5) Protein A
6) Hyaluronidase
7) Lipase
Term
What are the _ diseases caused by Staph Aureus?
Definition
1) Boils
2) Impetigo
3) carbuncles (pus filled lumps)
4) faruncles (severe carbuncles)
5) Food poisoning
6) Scalded skin syndrome
7) Toxic Shock Syndrome
8) Pneumonia
9) Bacteremia
7) Osteomyelitis
Term
What are the 6 symptoms of toxic shock syndrome?
Definition
1) high fever
2) hypotension
3) diarrhea
4) mental confusion
5) renal failure
6)scaling/desquamation of palms and soles
Term
How is staph aureus diagnosed?
Definition
1) Blood agar
2) Chocolate Agar
3) Selective Plates-oxacillin plate
4) NAATs
5) Catalase +
6) Coagulase +
7) betahemolytic
Term
What are the 4 treatments for staph aureus?
Definition
1) Oxacillin
2) Vancomysin
3) Cefazolin
4) Rifampin
Term
What is the gram of staph saprophyticus?
Definition
gram + cocci in clusters
Term
What diseases does staph saprophyticus cause?
Definition
UTI in women of child bearing age
Term
How is staph saprophyticus treated?
Definition
penicillin
Term
How can you differentiate between staph aureus and staph saprophyticus
Definition
staph aerus is coagulase +
staph saprophyticus is coagulase -
Term
What is the gram of Nisseria gonorrhea?
Definition
Gram - dipplococci intracellular
Term
What other bacterial infection often occurs with GC?
Definition
Chlamydia (50% chance)
Increased susceptibility to get HIV
Term
What are the 5 characteristics of Cervicitis in GC?
Definition
1) Abnormal mucopurulent vaginal discharge
2) easy cervical bleeding
3)intermenstrual bleeding
4) dysuria (painful urination)
5) lower abdominal pain
6) dyspareunia (painful sex)
Term
What are the 6 diseases caused by GC in women?
What percentage show no symptoms?
Definition
1) cervicitis
2) Pelvic Inflammatory Disease (PID)
3) Fitz-Hugh-Curtis Syndrome (PID in abs)
4) Bartholinitis
5) Vulvovagintis
6) Proctitis

50% asymptomatic
Term
What are the 5 diseases caused by GC in men?
What percentage show no symptoms?
Definition
1) urethritis (mucopurulent dischange)
2) Dysuria
3) Epididymitis
4) Prostatitis
5) PRoctitis

10% asymptomatic
Term
What are 3 infections by GC not directly associated with women or men?
Definition
1)oropharyngeal
2) opthalmia neonatorium (baby eye infection)
3) Disseminated gonococcal infection (in blood)
Term
What are the 6 ways you can diagnose GC?
Definition
1) Urethral swab/vaginal swab
2) Chocolate agar based plates
3) Jembec plate
4) DNA probe
5) NAATs
6) Gram stain showing intracellular
Term
How is GC treated?
Definition
1) Quinolones which have increased resistance
2) Cephalosporins
Term
What is the gram stain of nisseria menigiditis?
Definition
gram - diplococci not intracellular
Term
How is nisseria minigiditis transmitted?
Definition
person to person
Term
What are the 3 virulence factors of nisseria menigiditis?
Definition
1) capsule
2) pili
3) ALOT of lipid A
Term
What are the 6 diseases caused by nisseria meningiditis?
Definition
1) meningococcemia (in blood)
2) meningitis
3) Pharyngitis
4) Sinusitis
5) Primary pneumonia
6) DIC
Term
When diagnosing a spinal tap for nisseria meningiditis, what are the two conditions you look for?
Definition
1) lots of organisms with no WBCs
2) no organisms with lots of WBCs
Term
How is nisseria meningiditis treated?
Definition
penicillin
Term
What is the gram stain of moraxella catarrhalis?
Definition
gram - cocci in pairs (not intracellular)
Term
What four diseases are caused by moraxella catarrhalis?
What must exist for them to occur?
Definition
1) bronchitis
2) sinusitis
3) otitis Media
4) pneumonia

COPD or other pre-existing condition
Term
How is moraxella catarrhalis diagnosed?
Definition
Jembec plate
Term
What is the gram of Haemophilus
Definition
gram - pleomorphic rod
Term
What disease is caused by haemophilus egyptius?
Definition
conjunctivitis
Term
What disease is caused by haemophilus aphrophilius?
Definition
endocarditis
Term
What disease is caused by haemophilus pareinfluenzae?>
Definition
upper respiratory infection
Term
What disease is caused by haemophilus duereyi
Definition
STD
Term
How is haemophilus influenzae transmitted?
Definition
person to person via aersol
Term
What are the four diseases caused by haemophilus influenzae?
Definition
1) meningitis (HIBHIV vaccine stops most)
2) Cellulitis
3) otitis media
4) Respiratory infection
Term
How is haemophilus influenzae diagnosed?
Definition
1) Grows on chocolate agar
2) satelites around staph on a blood agar
Term
Why does haemophilus influenzae require chocolate agar plates and not blood?
Definition
haemophilus influenzae needs broke down cell parts to work
Term
What is the treatment for haemophilus influenzae?
Definition
cephalosporins
Term
What is the gram of bordetella pertusis?
Definition
gram - rod
Term
What are the stages of whooping cough?
Definition
1) catarrhal- runny nose
2) paroxysmal- whooping cough/cyanosis
3) convalescent- get better or die
Term
How is bordatella pertusis transmitted?
Definition
person to person via upper respiratory tract
Term
Who are the two groups at risk for bordatella pertusis?
Definition
1) infants before all 3 vaccinations
2) children 8-11
Term
What is the virulence factor of bordatella pertusis?
Definition
toxin
Term
What are the 3 diagnosis for bordatella pertusis?
Definition
1) REgan-Lowe plate with charcoal to absorb toxin (grows as mercury droplets)
2) Flourescent staining
3) PCR
Term
What is the best sample for bordatella pertusis?
Definition
nasopharyngeal swab transported in tube with charcoal
Term
What is the gram of shigella?
Definition
gram - rod
Term
What are the two types of shigella and where are they found?
Definition
Shigella dysenteriae- 3rd world
Shigella flexneri-USA
Term
Does shigella have a high or low infecting dose?

Does shigella incubate for long or short time?
Definition
low and short
Term
How is shigella transmitted?
Definition
fecal-oral
Term
What is the virulence factor of shigella?
Definition
Shigella toxin that binds to ribosome to stop protein synthesis
Term
What are the 5 symptoms of shigella?
Definition
1) diarrhea
2) Gastroenteritis
3) Nausea
4) Vomiting
5) Cramps
Term
What is the treatment for shigella?
Definition
hydration
Term
What is the gram of salmonella
Definition
Gram - rod
Term
Does salmonella have a high or low infecting dose?
Definition
high
Term
What are the two virulence factors of salmonella?
Definition
1) cytotoxins
2) enterotoxins
Term
What is one specific type of salmonella and what does it cause
Definition
Salmonella typhi- thypoid fever
Term
What are the 4 symptoms of salmonella?
Definition
1) Gastroenteritis
2) Fever
3) diarrhea
4) loss of appetite
Term
How is salmonella treated?>
Definition
quinolones
Term
What is the gram stain for Yersinia Entercolitica?
Definition
gram - rod
Term
What are the virulence factors of yersina entercolitica?
Definition
1) enterotoxin
2) betalactamase
Term
What are sources of yersina entercolitica?>
Definition
cattle, pig, deer, birds, unchlorinated water
Term
What are the 6 symptoms of yersina entercolitica?
Definition
1) actue entercolitis
2) Actue gastroenteritis
3) acute mesenteric lymphadenititis
4) Mimics appendicitis
5)diarrhea
6) fever
Term
What bacteria causes symptoms that mimic appendicitis?
Definition
Yersina entercolitica
Term
What plate is used to diagnose yersina entercolitica?
Definition
CIN agar
Term
What is the treatment of yersina entercolitica?
Definition
1) usually clears on own
2) aminoglycosides
3) quinolones
Term
What is the gram of Helicobacter pylori
Definition
gram - spiral
Term
What 3 diseases are caused by helicobacter pylori?
Definition
1)stomach ulcers
2) dyspepsia
3) gastritis
Term
How is heliobacter pylori diagnosed?
Definition
1) Culture of antrad biopsy
2) CLO test (red=+, green=-) testing for urease which makes stomach acid not harmful to bacteria
3) silver stain
4) serology
5) antigen test on stool
Term
What is the treatment of helicobacter pylori?
Definition
Triple Therapy with protein pump inhibitor
Term
What is the gram stain of campylobacter?
Definition
gram - curved rod
Term
What is very specific about campylobacter's growth needs
Definition
1) microaerophillic
2) grows at elevated temp 42C
Term
What is the source of campylobacter?
Definition
kenneled/house pets, undercooked poultry
Term
What are 4 symptoms of Campylobacter?
Definition
1) diarrhea
2) abdominal cramps
3) Fever
4) Vomiting
Term
What is used to treat campylobacter?
Definition
Erythromycin
Term
What is important to note about patients taking erythromycin?
Definition
it causes stomach upset so many people stop using it prematurely
Term
What is the gram for pseudomonas aeruginosa?
Definition
gram - rod
Term
What does pyoveradin do? which bacteria produces it?
Definition
1) turns wounds green
2) psuedomonas aeruginosa
Term
What are the 6 virulence factors of psuedomonas aeruginosa?
Definition
1) Capsule
2) Pili
3) Lipid A
4) Exotoxin A
5) Elastase
6) Cytotoxin
Term
What are teh 5 Resistance factors of psuedomonas aeruginosa?
Definition
1) beta-lactamase
2) decreased permeability
3) altered transpeptidase
4) enzymatic hydrolysis
5) Altered DNA Gyrase
Term
What preexisting condition has a 100% rate of having psuedomonas aeruginosa?
Definition
Cystic Fibrosis
Term
What are the 7 diseases caused by psuedomonas aeruginosa?
Definition
1) UTI
2) Infections of wounds, burns, ulcers
3) external otitis
4) Septicemia
5) Eye infections
6) Lung infections
7) hot tub folliculitis
Term
What bacteria grows clear on a MacConkey agar?
Definition
Psuedomonas aeruginosa
Term
What is the treatment for psuedomonas aeruginosa?
Definition
None
Term
What is the gram of mycobacteria leprae?
Definition
gram + rod
Term
What are the virulence factors of mycobacteria leprae?
Definition
1) Cell wall Waxes
2) Cord Factor
Term
How is mycobacteria leprae diagnosed?
Definition
1) cant be cultured
2) based on symptoms
Term
What general disease is caused by mycobacteria leprae?
Definition
Hansens disease
Term
What are the two symptom (diseases) classes of mycobacteria leprae?
Definition
1) Lepromatous- disfigures face, ears, etc, invades PNS for sensory loss
2) Tuberculoid- acts like TB
Term
What is the gram stain of mycobacteria tuberculosis?
Definition
gram + rod
Term
How many cases of mycobacteria tuberculosis are dormant?
Definition
95%
Term
What are the 2 virulence factors of mycobacteria tuberculosis?
Definition
1) Cell wall waxes
2) cord factor
Term
How is mycobacteria tuberculosis trasmitted?
Definition
infectious droplets
Term
where to mycobacteria tuberculosis develop in the body>
Definition
inside macrophages in the lungs
Term
What are the symptoms of mycobacteria tuberculosis?
Definition
1) Hilar and mediastinal lymph node swelling
2) granulomas in lungs
3) long history of weight loss, night sweats, coughing, malase
Term
What group of people are most likely to carry mycobacteria tuberculosis?
Definition
Foreign born people who sometimes have the vaccine giving them a positive skin test
Term
What diagnostic tests are used for mycobacteria tuberculosis?
Definition
1) skin test
2) Culture of sputum
3) Acid Fast Stain
4) Quantification on gold
Term
What is the treatment for mycobacteria tuberculosis?
What is one side effect to look for?
Definition
Directly observed therapy- 3 drug combo for 9 months to a year watching the patient take it
2) potential liver damage
Term
What does MOTT Stand for?
Definition
Mycobacteria Other Than TB
Term
What MOTT is very common among Aids patients?
Definition
Mycobacteria avium-intracellular (MAC,MAI)
Term
What are the symptoms of Mycobacteria avium-intracellular?
Definition
Looks like TB, pneumonia, bacteremia, lessions in bone marrow, spleen, and liver
Term
What is the treatment of mycobacteria avium-intracellular?
Definition
4-5 drugs, never cured only contained
Term
What MOTT causes cervical lymphadentis?
Definition
Mycobacteria scrofulaceum
Term
WHat MOTT is found in fish tanks and causes wound infections?
Definition
Mycobacteria marinum
Term
What is the gram of Yersina Pestis?
Definition
Gram - pleomorphic rod
Term
What are the 5 virulence factors of Yersinia Pestis?
Definition
1) pesticin- kills other organisms
2) Fibrinolysin- lyses fibrin clots
3) coagulase
4) Capsule
5) Lipid A
Term
What is the source of Yersinia pestis?
What is the reservoir?
Definition
1) fleas
2) rats
Term
What are the two disases caused by yersinia Pestis?
How are they transfered?
What is their fatality risk?
Definition
1) Bubonic plage
a) isolated in humans
b) transfered flea to human
c) not that fatal
2) Pneumonic Plague
a) bubonic that spread to lungs
b) highly infectious person to person
c) fatal
Term
What plate does yersina pestis grow on?
Definition
blood agar
Term
What is the gram for Brucella?
Definition
gram - rod
Term
What are 5 characteristics of Brucella?
Definition
1) microaerophillic
2) grows on blood agar in 2-4 days
3) Biochemicaly inert
4) Highly infectious
5) Grows intracellularly
Term
What is the source of Brucella
Definition
cattle, dog, pigs, goats, unpasteurized milk
Term
Who is at greater risk for Brucella infections?
Definition
1) Vets
2) abaitors (slaughterhouse workers)
Term
What are 3 symptoms of Brucella?
Definition
1) Conjunctivitis
2) Enlarged lymph nodes
3) osteomyelitis
Term
What 3 drugs can be used to treat Brucella?
Definition
1) Aminoglycosides
2) Gentamicin
3) Tetracycline
Term
What is the gram stain of Francisella tularensis?
Definition
gram - rod
Term
What are 5 characteristics of Francisella tularensis?
Definition
1) Nonencapsulated
2) Stains bipolar (red, clear, blue)
3) requires special media to grow
4) grows in 1-4 days
5) biochemically inert
Term
What disease is caused by francisella tularensis?
Definition
tularemia
Term
What are the 4 ways that francisella tularensis is transmitted?
Definition
1) Anthropod bites
2) Contact with infected tissues/blood (hunters)
3) Ingestion
4) Inhalation (run over bunny cutting grass)
Term
What is the source for Francisella tularensis?
What is the reservoir?
Definition
1) ticks
2) Rabbits
Term
What are 3 diagnostic tests for francisella tularensis?
Definition
1) Serology- most used
2) Culture of lymph nodes
3) Tissue IFA
Term
What are 4 drugs used to treat Francisella tularensis?
Definition
1) Streptomycin
2) Chlorophenicol
3) Genatmicin
4) Tetracycline
Term
What is the gram stain of Pasteurella multicida
Definition
Gram - rod
Term
What are 2 characteristics of Pasteurella multicida?
Definition
1) Capsulated
2) unique cell wall
Term
What are the sources of pasteurella multicida?
Definition
scratches from cats, swine, dogs, rats
Term
What type of infections does pasteurella multicida cause
Definition
1) wound infections
2) chronic skin ulcers
3) Osteomyelitis
Term
What is the treatment for pasteurella multicida?
Why is it unique?
Definition
1) Penicillin
2)due to the unique cell wall, it is the only gram - that can be treated with penicillin
Term
What is the gram stain of Bartonella henselae?
Definition
gram - rod
Term
What 5 diseases can be caused by Bartonella henselae?
Definition
1) Cat Scratch Fever
2) Bacillary Angiomatosis
3) Relapsing Fever w/ bacteremia
4) Peliosis hepatis
5) Endocarditis
Term
What are teh sources for Bartonella henselae?
Definition
scratches from cats or contact with cat feces
Term
What are the symptoms of cat scratch fever 3-10 days after scratch?
after 2 weeks?
Definition
1) primary pustule at inoculation site
2) a)regional lymphadenopathy
b) Low grade fever
c) Headache/muscle ache
d) Cutaneous rash
Term
What are two tests used to diagnose Bartonella henselae (Cat Scratch Fever)?
Definition
1) Clinical (tender nodes that develop over 3-4 weeks)
2) Histopathology (silver stain on lymph biopsy)
Term
Why are skin tests no longer used for Bartonella henselae?
Definition
Used human material
Term
Why are serological tests no longer used for Bartonella henselae?
Definition
many people have the antibodies but not the infection
Term
What is the treatment for Bartonella henselae?>
Definition
None clears on own
Term
What is the gram stain for Treponema Pallidum?
Definition
gram - spiral "Spirochete"
Term
What disease is caused by Treponema Pallidum?
Definition
syphilis
Term
What are the stages of Treponema Pallidum?
Definition
1) Primary
2) Secondary
3) Latent
4) Tertiary
Term
What is the RPR and FTA of Treponema Pallidum during the secondary stage?
Definition
RPR=99%(peak before decrease) FTA=100%
Term
Why is it important to diagnosis syphilis during the primary or secondary stage?
Definition
during the latent stage RPR decreases till undetectable... Asymptomatic
Term
What are some symptoms of tertiary syphilis?
Definition
Gummas, CNS infections (dementia)
Term
What are the tests for syphilis?
Definition
1) VDRL- only done in reference labs for tertiary syphilis
2) Rapid Plasma Region (RPR)- agglutination test with dilutions
3) FTA-ABS- looks for syphilis antibodies (once positive always positive)
4) Immunofluorensce- for primary syphilis(scrap ulcer for organisms)
Term
Why can RPR for syphilis be a false negative?
Definition
nonspecific can be positive for viral infections (VZV, Hepatitis, EBV) autoimmune (rheumatoid arthritis, lupus), and pregnancy
Term
What is the treatment for syphilis?
Definition
penicillin
Term
Why is Chlamydia impossible to gram stain?
Definition
it is an almost bacteria
Term
What are the 4 stages of the Chlamydia life cycle?
Definition
1) infectious elementary bodies infect a macrophage
2) Multiply in macrophage
3) Lyse macrophage to release many elementary bodies
4) repeat
Term
What STD occurs often with Chlamydia?
Definition
Nisseria gonorrhea
Term
What are 7 symptoms of chlamydia in males?
Definition
1)mucopurulent discharge
2) Dysuira
3) Conjunctivitis
4) Urethritis
5) Postatitis
6) Reiters syndrome
7) Epididymitis
Term
What are symptoms of chlamydia in females?
Definition
1) mucopurlent dischange
2) edamatous (swollen with fluid) cervical ectopy with erthemia (red and painful)
3) conjunctivitis
4) urethritis
5) cervicitis
6) proctitis
7) PID
8) endometritis
9)Salpinitis
10) Perihepatitis
11) Reiters syndome
12) Infertility
13) Ectopic pregnancy
Term
what tests are used for Chlamydia?
Definition
1) sample of dischange
2) Culture (100% accurate used for sexual abuse cases only)
3) NAAT
Term
Why is screening for Chlamydia important?
Definition
can decrease incidence of PID by 50% and help prevent its spread to others
also it is usually asymptomatic
Term
What is the treatment for Chlamydia?
Definition
fluoroquinolones
Term
What are the four types of e.coli?
Definition
1) Enterotoxigenic
2) Enteroinvasive
3) Enterohemorrhagic (E.col0157h7
4) Enterophathogenic
Term
What test can be used to find ecoli0157h7?
Definition
ELISA
Term
What is the treatment for e.coli?
Definition
cephalosporins
aminoglycosides
Term
What is the scientific name for an anaerobic infection?
Definition
endogenous
Term
What are the 6 clues you have an anaerobic infection?
Definition
1) Foul odor
2) gas in tissue
3) Tissue necrosis
4) Black discoloration
5) unique morphology
6) gram + stain, but no agar growth
Term
What causes many OBGYN anaerobic infections
Definition
IUD, bacteria adhere to it
Term
What group is most likely to suffer from an anaerobic thoracic infection?
Definition
alcoholics that aspirate saliva leading to lung infections
Term
What are the 7 virulence factors of anaerobic infections?
Definition
1) Necrotizing enzymes- cause gas gange green
2) Phospholipase
3) Hemolysin- lyses RBCs
4) Protease
5) Hyaluronidase
6) Enterotoxins- gastroenteritis
7) Spore Formation
Term
What are the two best samples for anaerobic infections?
Definition
1) aspirates
2) Tissue samples
Term
How do you transport anaerobic organisms?
where do they grow?
Definition
1) in syringe
2) anaerobic enviroment machine
Term
What is the gram stain for Clostrida?
Definition
Gram + rod
Term
What disease does Clostridia perfringes cause?
Definition
food poisoning
Term
What disease does Clostridia botulinum cause?
Definition
botulism
Term
What disease does Clostridia septicum cause?
Definition
Indicates GI cancer
Term
What disease does Clostridia tetani cause?
Definition
tetnus
Term
What are the two parts of a Clostrida difficle bacteria?
Definition
1) spore
2) vegetative
Term
What are sources of Clostrida difficle?
Definition
Human feces, hospitals, soil and water
Term
What are the virulence factors of clostridia difficle?
Definition
toxin A and B
Term
How does clostridia difficle infect?
Definition
antibiotics kill all normal flora so clostrida difficle can florish.
4-10 after starting antibiotics
up to two weeks after stoping them.
Term
What are the effects of toxin A produced by the vegetative part of clostridia difficle?
Definition
causes cells to lyse and unable to control water movements to cause intense diarrhea
Term
How is clostridia difficle transmitted?
Definition
fecal oral
aerosols by spores
Term
What are psuedomembranes, what bacteria causes them?
Definition
1) membranes that obliterate the bowel to inhibit function
2) clostridia difficle
Term
What are 4 symptoms of clostrida difficle?
Definition
1) abdominal pain
2) watery diarrhea
3) Ileus- blocked bowel
4) WBCs in stool
Term
What are the two best ways to diagnose clostrida difficle?
Definition
1) Immunoassay looking for toxin a and b only 70% right
PCR
Term
Who is at risk for clostrida difficle?
Definition
elderly have a high mortality, and anyone in a longterm care facility or hospital.
Term
What are the 4 types of clostrida difficle?
Definition
1) community onset
2) community associated
3) hospital onset
4) long term care onset
Term
What is the treatment of clostrida difficle?
Definition
1) Stop antibiotics
2) Anti-C difficle drugs- metronidazole(never vancomysin for it can lead to VRE)
3)Fecal transplant
Term
What is the shape of clostridia difficle in a gram stain
Definition
box car
Term
What is the gram stain of legionella pneumophilia?
Definition
gram - rod
Term
Is legionella pneumophila aerobic or anaerobic?
Definition
aerobic
Term
What is the source of legionella pneumophilia?
Definition
contaiminated water in AC for large bldgs
Term
Who is at risk for a legionella pneumophilia infection?
Definition
1) Vets
2) Those with impaired respiratory defense (smokers, alcoholics, elderly)
Term
What are the 6 diseases caused by legionella pneumophilia?
Definition
1) Multilobar pneumonia
2) Extensive X ray findings (looks obliterated
3) Nonproductive cough (no sputum)
4) No pathogen seen in culture/no gram stain
5) No response to tradtition therapy
6)Extrapulomary findings (kidney/liver failure)
Term
What is test for legionella pneumophilia?
Definition
1) Urinary antigen test (grows like ground glass)
Term
What is the treatment for legionella pneumophilia?
Definition
erythromycin because it can get into the macrophages were it is
Term
What are the four classes of fungi?
Definition
1) Zygomacetes
2) Ascomycetes
3) Basidiomycetes
4) Deuteromycetes
Term
What characteristics separate 1) zygomycetes, and 2) Dueteromycetes from the other fungi
Definition
1) as aseptate
2) are imperfect fungi "no sexual reproduction"
Term
What are the types ways of which poisonous mushrooms onset their syptoms?
Which is worse and why?
Definition
1) Rapid onset, delayed onset
2) delayed is worst because you cant easily ID what caused it
Term
What fungi is penicillin derived from?
Definition
Penicillium
Term
What are the 5 types of fungi (how they cause disease)?
Definition
1) Allergic-allergies
2) Mycotoxicosis- ingest toxin
3) Superficial- remain on skin
4) Subcutaneous- skin and lymph
5) Systemic- invasive everywhere
Term
State the area of infection for the following Tineas.
1)capitis
2) corpotis
3) barbae
4) unguium
5) cruris
6) pedis
Definition
1) scalp
2) body
3) beard
4) unguium
5) groin
6) foot
Term
What is the food source for dermatophytes?
Definition
keratin
Term
What are the two symptoms of a generic dermatophyte infection?
Definition
1) itching
2) inflammation
Term
What are two clinical methods for dermatophyte infections?
Definition
1) KOH test looking for fungal elements
2) removal of infected nail
Term
What diseases does Trichophytan rubrum cause?
Definition
1) Athletes foot (tinea pedis)
2) Nail infections
3) rare endothrix and exothrix hair infections
Term
What are the symptoms of Trichophytan rubrum?
Definition
1) Itch
2) White chalky flaky skin
Term
What color does trichophytan rubrum grow on media?
Definition
ruby red
Term
What disease is caused by Trichophytan tonsurans?
Definition
tinea capitis- endothrix
Term
What are the symptoms of trichophytan tonsurans?
Definition
1) inflammation
2) white powdery looking scalp
Term
What are the sources of trichophytan tonsurans?
Definition
1) comb
2) contact with infected person
Term
What color does trichophytan tonsurans grow on media?
Definition
butterscotch
Term
What two trichophytan are anthropophilic?
Definition
trichophytan rubrum and tonsurans
Term
What trichophytan is zoophilic?
Definition
trichophytan mentagrophytes
Term
What are the symptoms of trichophytan mentagrophytes?
Definition
1) inflammation
2) lesions on scalp
3) kerions (pus lesions 2ndary to bacteria infections)
Term
What disease is caused by micosporum canis?
Definition
ring worm
Term
What color does micosporum canis grow on media?
Definition
lemon yellow
Term
What fungi are dimorphic?
Definition
1) sporotrichosis
2) Histoplasmosis
3) Blastomycosis
4) Cryptococcis
Term
What fungi is a subcutaneous mycosis?
Definition
sporotrichosis
Term
What is the source of sporotrichosis?
Definition
sporothrix schenkii from soil an rose thorns
Term
Cigar bodies are formed by what fungi?
Definition
sporotrichosis
Term
What are the two treatments for sporotrichosis?
Definition
1) KI
2) Hydrotherapy
Term
What type of mycosis are oppertunistic infections (fungi)?
Definition
systemic mycosis
Term
What is the treatment for histoplasmosis?
Definition
amphotericin B
Term
Where is histoplasmosis located?
Definition
Mississippi River Valley
Term
What is the source of histoplasmosis?
Definition
soil, forest floor, saves
Term
Where is blastomycosis located?
Definition
Minnesota, Wisconsin
Term
What is the source of blastomycosis?
Definition
soil and ponds
Term
What fungi forms spherules in the body?
Definition
cocci
Term
What are the 8 stages of viral infection/replication?
Definition
1) adsorption- adhere to cell
2) penetration
3) uncoated- capsid removed
4) transcription
5) translation
6) processing
7)replication
8) assembly
Term
Compare envelope and nonenvelope viruses
Definition
envelope- bud from host cell and dont survive well in enviroment

nonenvelope- lyse host cell, live well in enviroment
Term
What are the 3 layers of a virus?
Definition
1) protein/carb group projections
2) capsid- protein coat around DNA/RNA
3) DNA/RNA
Term
What are the 6 viruses that cause Encephalitis?
Definition
1) California encephalitis
2) St. Louis encephalitis
3) Western Equire encephalitis
4) Herpes
5) Mumps
6) Enterovirus
Term
What is the source of aspergillosis?
Definition
compost
Term
What fungus causes a fungus ball to develop??
Definition
aspergillosis
Term
Uncontrolled diabetes can lead to what fungal infection?
Definition
zygomycosis
Term
Where does zygomycosis start then finish
Definition
nose (inhalation)->eye->brain
Term
When is zygomycosis diagnosed?
Definition
post mortum
Term
What infection does candidosis cause?
Definition
Thrush- white patch on tongue
Term
What are the two types of people most likely to get thrush?
Definition
1) those on antibiotics
2) Aids (early sign)
Term
Where does candidosis desseminate to?
Definition
kidney
Term
What is the source of cryptococcosis
Definition
inhalation of spores from bird poop
Term
What are the 7 viruses associated with gastroenteritis?
Definition
1) rotavirus
2) Hepatitis A
3) Adenovirus
4) Calcivirus
5) norwalk virus
6) norovirus
7) sapovirus
Term
Is rotavirus a DNA or RNA virus?
Definition
RNA
Term
What is the transmission method for rotavirus?
Definition
fecal-oral
Term
What are the 4 sypmtoms of winter vomiting disease (rotavirus)
Definition
1) vomit
2) diarrhea
3) green stool from bile
4) dehydration->hospital
Term
What is the treatment for rotavirus?
Definition
hydration
Term
What is the incubation period for Hepatitis A?
Definition
30 days with symptoms on and off
Term
What are the infection stages for Hepatitis A?
Definition
1) shed in stool
2) increase in symptoms
3) spike in liver enzymes
4) increase and decrease of IgM and anti-HAV
Term
How is Hepatitis A diagnosed?
Definition
increased liver enzymes and IgM Test
Term
What are the 3 highest sources of the Hepatitis A virus in the body?
Definition
1) feces
2) serum
3) saliva
Term
What are the 3 transmission methods for Hepatitis A?
Definition
1) personal contact- sex
2) contaminated food/water
3) Blood exposure- IVs and transfusions
Term
What are the 3 problems with the Hep A vaccine?
Definition
1) greatly varies in efficacy
2) doesnt always protect you
3) wears off
Term
Who gets the Hep A vaccine?
Definition
travelers, gay men, drug users
Term
What hepatitis causes liver disease?
Definition
Hepatitis B
Term
What are the high sources, medium sources, and low sources in the body for Hep B?
Definition
High- blood, serum, wound exudates
Medium- semen, vaginal fluid, saliva (STD)
Low- urine, feces, sweat, tears, breast milk
Term
WHat are the 3 ways Hepatitis B is transmitted?
Definition
1) Sexual
2) Parenteral (needles)
3) Perinatal (birth Canal)
Term
What is the incubation period for Hepatitis B?
Definition
60-90 days
Term
Why is the vaccine for Hep B very important
Definition
15-25% die prematurely due to chronic infection
Term
What is the difference in the serological stages of Acute vs chronic Hep B?
Definition
in acute the HBsAG increases then decreases, while in chronic it remains high
Term
Which Hepatitis causes the most deaths and is related to liver cancer?
Definition
Hepatitis C
Term
What type of virus is Repsiratory Syncytial Virus (RSV)
Definition
enveloped RNA
Term
What diseases does RSV cause in young children
Definition
croup and pneumonia
Term
What are the symptoms of Type A flu?
Definition
fever and body ache for 3-5days
cough and lack of energy - 2 weeks
Term
What are the two transmission methods for Typa A flu?
Definition
1) person to person via cough
2) fomites
Term
What do the H and N in a flu name stand for and how many options are there
Definition
h- hemaggultinites 15
n- neuraminadase 9
Term
What is antigenic drift?
Definition
small changes over time in H and N causeing new strands to replace the older ones- need for annual vaccine
Term
What is antigenic shift?
Definition
Abrupt changes and reassortment in H and N creating a novel pandemic flu.
ex) swine, spanish, asian, hong kong flu
Term
What are the 3 animals involved in antigenic drift mixing?
Definition
1) bird->pig->human
Term
What is a sentinel?
Definition
physicans who look for flu-like symptoms and send samples to state to keep track of what flus are circulating
Term
What does a typical flu vaccine contain?
Definition
1 A, 1B, and 1 novel strain
Term
What are the 4 viruses that fall under Herpes Virus?
Definition
1) Herpes complex I and II
2) Varicella Zoster
3) Cytomegalovirus
4) Epstein Barr Virus
Term
What 6 diseases are caused by Herpes Simplex>
Definition
1) gingiovostomatitis- seen first
2) keratoconjunctivitis
3) herpetic whitlow - dentists
4) adult necrotizing encephalitis
5) neonatal encephalitis
6) herpes genitalis- STD
Term
Where does the herpes virus reside in the body?
Definition
Nerves for life
Term
How do you diagnose herpes?>
Definition
clinical
Term
What are the 3 diseases caused by Varicella Zoster?
Definition
1) chicken pox
2) pneumonitis in immunosuppressed
3) shingles
Term
What is the clinical look of chicken pox>
Definition
dew drops on a rose petal
Term
Why do all health care providers need to get a varicella zosters vaccine?
Definition
causes pneumonitis in immunosuppressed
Term
What are the 6 stages of replication for varicella zoster?
Definition
1) attachment to host cell
2) entry into host cell
3) uncoating
4) replication of parts
5) assembly of virus
6) escape from host cell
Term
What two diseases are caused by entameba histolytica?
Definition
1) acute colitis- abdominal pain, cramps, diarrhea with blood
2) fulminate amebic colitis- bloody diarrhea, liver abscesses
Term
What does the stool of a person with entameba histolytica look like?
Definition
mushy, water, foul stool
Term
How is entameba histolytica transmitted?
Definition
fecal-oral
Term
What is the source of entameba histolytica?
Definition
water
Term
What are the two major groups of parasites?
Definition
1) Protozoan- single celled
2) Helminths- multicelled
Term
What major parasite group does entameba histolytica fall under?
Definition
protozaon, ameba
Term
What major parasite group does giardia fall under?
Definition
Protozoan, flaggellate
Term
What disease does giardia cause?
Definition
"beaver fever"
Term
What are the symptoms of beaver fever?
Definition
1) acute giardiosis
nausea and GI discomfort
2) chronic reccurent diarrhea- headache, myalgia, and weight loss for up to two years
Term
What is the stool like for a giardia infection?
Definition
explosive, foul smelling, frothy diarrhea for 3-4 days
Term
Why is giardia poop frothy?
Definition
organism inhibits absorption of lipids
Term
What is the source of giardia?
Definition
water
Term
What is the most common way to test for parasites that are protozoan?
Definition
Microscope
Term
What parasite has no cyst stage?
Definition
Trichomonas
Term
What 5=4 diseases does trichomonas cause?
Definition
1) vaginitis
2) cystitis
3) urethritis- STd
4) burning/itchy discharge
Term
What parasite can be seen in urine occasionally?
Definition
Trichomonas
Term
What is a wet prep?
Definition
swab, saline, microscope
Term
What are the two hosts needed for a Cestode parasite?
Definition
1) Intermediate host (cow)- parasite develops into larva
2) Definitive host (person)- larva develop into adult parasite
Term
What are the 7 stages of a cestode life cycle?
Definition
1) adult worm in instintes of D host
2) eggs are passed in poop of D host
3) eggs are eaten by I host
4) Larva develop in tissues of I host
5) D host eats flesh of I host
6) scolex (head of worm) attaches to D host intestines
7) adult worm grows, repeat cycle
Term
What is the science name for tape worm?
Definition
Tinea saginata
Term
What is the source of tapeworm?
Definition
undercooked beef
Term
What parasite is hermaphroditic
Definition
1) tape worm
Term
What parasites require male and females?
Definition
1) Ascaris
2) Pin worm
Term
What does a tape worm have that helps it attach to the intestinal wall
Definition
hooks and suckers
Term
How do you remove a tape worm infection?
Definition
remove head from intestinal wall
Term
The parasite ascaris falls under what major group of parasites?
Definition
Helminths, nematode
Term
What does the parasite ascaris look like?
Definition
white earth worm minus the bandaid
Term
What is the source of the parasite ascaris?
Definition
soil
Term
What is the 5 stages of the life cycle of ascaris?
Definition
1) unembryonated eggs in soil mature for 2 to 3 weeks
2) ingest eggs, larva hatch in the duodenum and leave via the bloodstream to liver->heart->lungs
3) larva break out of capillaries into alveolar space
4) larva go into trachea where they are swallowed?
5)relocate to the intestines to repeat cycle
Term
What major group of parasites does pinworm fall under?
Definition
Helminths, nematode
Term
Which parasite is bigger? ascaris or pinworm?
Definition
ascaris
Term
What parasite causes white itchy patches due to it crawling just under the skin?
Definition
scabes
Term
What kind of toxin does a brown recluse spider release?
Definition
cytotoxin leading to necrotic ulcer
Term
What kind of toxin does a black widow spider release?
Definition
neurotoxin leading to cramps
Supporting users have an ad free experience!