Term
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Definition
Coloring specimens with stains/dyes.
Increasing contrast and resolution. |
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Term
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Definition
| thing film of of microorganisms on slide |
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Term
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Definition
| Gently heated by passing slide, smear up, through flame. |
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Term
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Definition
| applying methyl alcohol (methanol) or formalin (formaldehyde in water) to the smear for one minute. |
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Term
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Definition
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Term
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Definition
| Kill the microorganisms, attach them firmly to the slide, and generally preserve their shape and size. |
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Term
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Definition
Cationic. Goes into it.
Stains acidic structures.
Work best under basic conditions. |
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Term
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Definition
Anionic. Repels.
Stain basic structures.
Work best under acidic conditions. |
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Term
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Definition
Determine size, shape, arrangement.
All cells appear the same color.
Methylene Blue
Crystal Violet
Safranin |
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Term
Differential Staining:
Gram Stain |
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Definition
Differentiates between Gram Positive(purple)
and Gram Negative(pink)
Key differences in cell wall structures of two groups of cells. |
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Term
Differential Staining:
Acid-Fast Stain |
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Definition
Differentiates from acid-fast (pink)
from non-acid-fast(blue)
Mycobacterium
Nocardia |
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Term
Differential Staining:
Endospore Stain |
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Definition
Dye is forced by heat into resistant endospores
Cell walls are impermeable to all chemicals.
Distinguishes between spores and cells they come from (vegetative cells) |
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Term
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Definition
| Reveal special cell structures |
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Term
Special Stains:
Negative(Capsule) Stain |
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Definition
Utilizes an acidic stain.
Stains the background but does not penetrate the negatively charged capsule. |
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Term
Special Stains:
Flagellar Stain |
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Definition
Dyes and mordants bind.
Increasing diameter and changing their color to make them visible.
Permit determination of number and location. |
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Term
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Definition
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Term
| Three Domains of Carl Woese |
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Definition
| Eukarya, Bacteria, and Archaea |
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Term
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Definition
| Shape, Size, Presence/Absence of structures. |
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Term
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Definition
Distinguishes prokaryotes with similar morphology
Only used for bacteria grown in lab. |
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Term
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Definition
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