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Microbiology 303
Exam 1
43
Biology
Graduate
02/10/2008

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Term
Syntropy
Definition

drive reaction forward

 

Syntrophy is an interaction between organisms that

combine their metabolic capabilities to catabolize

substances that individuals cannot catabolize alone.

Examples: PCBs and aromatic compounds 

Term
Termite Gut (genius name)
Definition

Trichomal protozoa

Term
Functions of Termite Gut
Definition

-Degrade cellulose to glucose

-Convert glucose to acetate and hydrogen

Term
Characteristics of Syntropy
Definition

•Degradation of fatty acids in rumen of termite

Syntrophs degrade lipids --- release H2

Methanogens (autotrophs, use CO2)

Term
Paulinella chromatophora
Definition

Endosymbiont

-an amoeboid that has taken on cyanobacterium

-provides photosynthate to amoeba

- closely related to free-living Synechococcus (cyanobacterial) species.

2 Endosymbionts in Plant cells = plastids and mitochondria

 

Term

Spherical (coccus) - give example

Definition

Staphylococcus aureus

- staph infections

-little things, pimples, skin infections, boils

-big things, pneumonia, meningitis, TSS

Term

rod shaped (bacillus) - give example

Definition

Azotobacter

form thick-walled cysts, and may produce large quantities of capsular slime

- gram negative bacteria

Term
Name two different

Spirochetes, and the disease they cause

Definition

Borrelia burgdorferi

(cause of Lyme’s disease)

 

Treponema pallidum

(cause of Syphilis)

Term

What is the first microbe grown in pure culture?

 

Which scientist discovered/researched it?

Definition

Bacillus anthracis

 

Robert Koch

Term
Mxycobacteria
Definition

Myxococcus xanthus

Gram negative rod-shaped bacteria

Term

tuberculosis

Definition
Mycobacterium
Term

cholera (Vibrio cholerae)

Definition

Gram-negative bacteria in contaminated food and water. 

Onset can be rapid - gets in lining of small intestine - excessive diarrhoea.

Term

phylogeny marker - 16S ribosomal RNA

Definition

Part of the ribosome

Required for translation of

mRNA into protein.

Present in all bacterial

(16S rRNA), archaeal and

eukaryotic cells (18S rRNA)

Term

Which domains are ester-linked (lipids)?

 

Ether-linked?

Definition

Ester- bacteria and eukaryotes

 

Ether- archaea

Term
Describe the difference in cell walls of the 3 domains
Definition

Eukaryotes - no cell wall or cellulose

 

Bactera- no cell wall, or gram +, or gram -

 

Archaea- proteins, pseudomurien, lattice

Term
Describe characteristics of Archaea
Definition

Very different

Extremophiles

High salt

High temperature

Acidic conditions

Basic conditions

Species also discovered at more normal conditions

Term
Which domain contains ALL pathogens?
Definition

Bacteria

Term
List major sources of soil nutrients
Definition

- chitin

-cellulose

-keratin

-lignin

Term

Describe some inner workings of tube worms (what they take up and what bacteria oxidize)

Definition

Worm uptakes CO2 and H2S using hemoglobin protein

 

•Bacterial symbiont is a sulfur oxidizer

 

Makes organic matter for worm

Term

What is the most important

thing in Microbe Mines?

Definition

FeS2 oxidation

Term
Mesophile
Definition

Organism that can live at hot or cold temperatures

Term
What are characteristics of mesophiles?
Definition

-ester-linked

 

-lipid bilayer

Term
What are characteristics of thermophiles?
Definition

ether-linked lipids

 

membrane MONO layer

Term
Wood Metabolism
Definition

1.Protozoa or bacteria break down cellulose

 

2. Methanogens or

homoacetogens use the hydrogen

 

3.  Without the breakdown of H2, cellulose wouldn't be broken down.

Term
Yeast (latin name)
Definition

Saccharomyces cerevisiae

Term

Pseudomonas putida

Definition

Ferments carbohydrates (on grapes), but it creates an acidic enviornment, yeast deacidifies the enviornment, allowing P. putida to survive.

(competition for carbohydrates)

Term

Quorum Sensing

Definition
The ability of bacteria to communicate and

coordinate behavior via small molecules; applies to

both intra and inter species communication.

Its purpose is to coordinate certain behavior based

on the local density of bacteria

-involves production of autoinducer

Term
What do bacteria use quorum sensing for?
Definition

Bacteria use quorum sensing for:

-Adaptation to availability of nutrients.

-Defense against other microorganisms which may compete for the same nutrients.

-Avoidance of toxic compounds potentially dangerous for the bacteria.

-Co-ordination of virulence in order to escape the immune response of the host to establish a successful infection

Term
What is a heterocyst?
Definition

-nitrogen-fixing cells made from cyanobacteria

-convert dinitrogen to nitrogen with nitrogenase

-need anaerobic environment

- it's physiology requires global change in gene expression

Term
What are the three compounds used for Quorum Sensing?
Definition
Oligopeptides, AHL, and AI-2
Term
Are oligopeptides Gram-Postive, Negative, or Both?
Definition
Postive
Term
Are AHL Gram-Postive, negative, or both?
Definition
Negative
Term

Are AI- 2 Gram-Postive, negative, or both?

Definition
Both
Term
Sctructurally, which autoinducer (quorum sensing compound) has a structure of two bound rings?
Definition
AI-2
Term
Sctructurally, which autoinducer (quorum sensing compound) has a structure of one ring plus a greasy end?
Definition
AHL
Term
Give an example of process regulated by Oligopeptides
Definition

-Staphylococcus aureus

Toxic shock and virulence

OR

Bacillus subtilis

Antibiotic production and sporulation

Term

Give a specific example of an AHL regulated process

Definition

-Vibrio Fischeri

Bioluminescence

OR

-Pseudomonas aeruginosa and Agrobacterium tumefaciens

 Virulence

Erwinia caratovora

Antibiotic and exoenzyme production

Term
Give an example of AI-2 or Lux regulated processes
Definition

Borrelia burgdorferi

Pleiotophic protein expression

OR

Salmonella typhi and Streptococcus mutans

Biofilm Formation

OR

Streptococcus pneumonae and Vibrio cholerae

Virulence

Term
What do you know about

Myxococcus?

Definition

Mound Formation!

Carnivourous rod-shaped Gram negative

bacteria

Obtain nutrients by secreting enzymes

that cause the lysis of other bacteria

If nutrients are scarce Myxococcus

produces fruiting bodies (A and C signaling)

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