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Microbiology 210 ICC Gavino
Unit 1
169
Microbiology
Undergraduate 2
06/08/2013

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Term
Cell Theroy
Definition
All organisms are composed of cells and new cells only come from preexisting cells
Term
Prokaryotic cell
Definition
DNA is not enclosed within a nucleus, does not have organelles enclosed by membranes.
Term
Prokaryotes
Definition
Are single celled (unicellular) organisms

Ex. Bacteria and Archaea
Term
Eukaryotic cell
Definition
DNA is inside a nucleus, has several organelles enclosed within membranes
Term
Eukaryotes
Definition
Can be unicellular or multicellular organisms
Ex. Protists (protizoa and algea), fungi, bacteria, and animals
Term
6 Basic Elements cells are build of
Definition
Co2, O2, hydrogen, nitrogen, phosphorus, sulfur
Term
DNA and its 4 nucleotide bases
Definition
-deoxyribonucleic acid, is a double stranded molecule made of adenine, thymine, guanine and cytosine
Hydrogen bonds hold the 2 strands together
Term
RNA and its 4 nucleotide bases
Definition
Ribonucleic acid is a single stranded molecule made of adenine, uracil, guanine and cytosine, which helpd DNA express traits and helps the ribosomes synthesize protein
Term
Genes
Definition
(Made of DNA) controls the traits of organisms, and have instructions for making proteins that are needed to express traits
Term
Genes (DNA) -> assisted by RNAs -> to make protiens -> to show traits
Definition
Term
Asexual Reproduction
Definition
A parent produces genetically identical clones
Term
Binary Fission
Definition
One cell devides in half
(Asexual)
Term
sexual reproduction
Definition
DNA from parents recombine to produce to genetically diverce offspring
Ex gametes or sex cells fused to form a zygote
Term
Theory of Evolution
Definition
Suvival of the fittest
Term
Photoautotrophs
Definition
use carbon dioxide as main source of carbon and solar energy to make their own food the process of photosynthesis
Ex cytanobacteria, algea and plants
Term
Heterotrophs
Definition
require different sources of organic carbon from food or organic nutrients
Term
the scientific method
Definition
1. observations and curiosity
2. hypothesis
3. experiment
4. data analysis
5. conclusion
Term
Louis Pasteur
Definition
- formed the basis of aseptic techniques
- fermentation
- pasteurization
- disapproved spontaneous generation
Term
Robert koch
Definition
-germ theory of disease
- pure cultures
- Microbacterium taburculosis
- vibro cholera
Term
Joseph Lister
Definition
Aseptic surgery
Term
Paul Ehrlich
Definition
-theory of immunity
- syphilis
Term
Alexander Fleming
Definition
- penicillin
Term
James Watson and Francis Crick
Definition
DNA structure
Term
Carlos Woese
Definition
three domain system of classification based on ribosomal DNA
Term
Robert Hook
Definition
first observation of cells
Term
Christian Gram
Definition
Gram staining procedure
Term
Antoni van Leeuwenhoek
Definition
first observation of live microorganisms
Term
Dimitri Iwanowski
Definition
observed the first virus
Term
Edward Jenner
Definition
first vaccine
Term
how do microorganisms contribute to our lives
Definition
- recycle
- sewage treatment
- bioremediation
- genetic engineering
- biotechnology
Term
What microbes recycle vital element and how?
Definition
bacteria and fungi decompose are we algae and cyanobacteria produce o2 and carbon dioxide
Term
What microbes contributes to sewage treatment and how?
Definition
Bacteria, to recycle H20
Term
Bioremediation
Definition
uses bacteria to degrade or detoxify pollutants in our environment such as oil spills and mercury
Term
Biotechnology (how are microbes used)
Definition
uses microbes to make food and chemicals
Term
Genetic engineering
(how are microbes used)
Definition
uses microbes to produce vaccines and enzymes (proteins)
Term
Normal Microbiota
Definition
microbes that normally grow on the surface or inside our body without causing disease
(symbiosis)
Term
Page 3 C dash 2!!!!!!!!!!!!!!!!!!1
Definition
Term
How are organisms grouped?
Definition
According to their characteristics
Term
Taxonomy
Definition
the science of classifying organisms; to give universal names; to identify newly discovered organisms; to understand how organisms are related
Term
Cell types that distinguish an organism
Definition
-Prokaryote cell
-Eukaryote cell
Term
Domain Bacteria
Definition
unicellular prokaryote; cell wall has peptidoglycan
Term
Domain Archaea
Definition
unicellular prokaryote; no peptidoglycan in cell wall; lives in extreme environments
Term
Hperthermophile
Definition
Class of archaea; like very hot places
Term
Methanogens
Definition
Class of archaea; like methane gas
Term
Halophile
Definition
Class of archaea; like very salty places
Term
Domain Eukarya
Definition
Can be unicellular or multicellular organisms
Ex. Protists (protizoa and algea), fungi, bacteria, and animals
Term
Taxonomic Hierarchy
Definition
domain, kingdom, phylum, class, order, family, genus, species
Term
Binomial
How is it written?
Definition
Two names that every organism is assigned; the genus and specific epithet (species)
-Both names are printed underlined or italicized
Term
What is a Eukaryotic species?
Definition
a group of closely related organisms that breed among themselves
Term
Genus
Definition
Is always a noun and is capitalized
-consists of species that differ from each other in certain ways but are related by descent
Term
Specific epithet (species)
Definition
Is usually an adjective and lowercase
Term
Family
Definition
consists of related genera
Term
Order
Definition
group of similar families
Term
Class
Definition
a group of similar orders
Term
Phylum
Definition
Related classes
Term
Kingdom
Definition
All related phyla
Term
Domain
Definition
All related Kingdoms
Term
Prokaryotic species
Definition
a population of cells with similar characteristics
Term
Clone
Definition
pure culture
-a population of cells derived from a single parent cell
Term
Strain
(how are they identified)
Definition
is when pure cultures of the same species are not genetically identical
-identified by numbers letters, or names that follow the specific epithet
Term
Viral species
Definition
population of viruses with similar characteristic (enzymes, genes, morphology) that occupy the same ecological niche (host).
Term
Morphology
Definition
based structural characteristics- shapes, size and arrangement of cells; used to identify eukaryotes because they are bigger than prokaryotes
Term
Differential Staining
Definition
Use dyes to identify bacteria based on their cell wall composition
ex gram positive or gram negative, acid fast stain
Term
Biochemical Tests
Definition
the presence of various enzymes (proteins) is used to differentiate bacteria
Term
Serology
Definition
tests the reaction of a microorganism (antigenic) with specific antibody (proteins)that is produced by an animals immune system
Term
Phage Typing
Definition
determines which phages (bacteriophages- bacterial viruses that usually cause lysis of the bacterial cell they infect) a bacterium is susceptible to.
Term
Fatty Acid Profiles
Definition
bacterial species can be identified based on the kinds of fatty acids the produce
Term
Flow Cytometry
Definition
tests the electrical conductivity of bacterial cells using lasers or light
Term
DNA Base Composition
Definition
the percentage of guanine and cytosine nucleotides of DNA can be used to classify organisms
Term
DNA Fingerprinting
Definition
an individual displays its unique DNA pattern based on its sequence---first the DNA is cut into fragments using restriction enzymes' then the different-sized fragments show a unique pattern on a agarose gel
Term
Polymerase Chain Reaction
Definition
makes many copies of a piece of DNA in order to identify species or strains this amplified piece of DNA may be a unique DNA sequence or gene
Term
Ribosomal RNA Sequencing
Definition
all prokaryotic and eukaryotic cells contain ribonucleic acids and ribosomes; the order of nucleotides of the RNA found in ribosomes is unique for a species
Term
Nucleic Acid (DNA)Sequencing
Definition
test the relatedness of individuals based on similarity of their DNA sequence; single strands of DNA from closely related organisms will bind (hydrogen boding between complementary bases) and form a double-stranded molecule
Term
Protozoa
Definition
unicellular, eukaryotic organisms
Term
Characteristics of Protozoa
1.Reproduction
2.Encystment
3.Nutrion
Definition
1. asexually; fission, budding, schizogony (multiple budding)
sexual; conjugation
2. Under certain adverse conditions some protozoa produce a cyst
3. Mostly aerobic heterotrophs
intestinal protozoa are capable of anaerobic growth
-all need large supply of H2O
Term
Trophozoite
Definition
the vegetative form of protozoa that fee and grow
Term
During Sexual Reproduction of Protozoa the DNA from the parents recombine to produce what?
Definition
Daughter Cells
Term
Domain; Eukarya, Kingdom; Protozoa, Phylum; Ciliophora, Amoebozoa, Apicomplexa, Archaezoa, Euglenazoa
Definition
Term
Domain Eukarya; Kingdom Protozoa; Phylum Ciliophora...
Definition
move and fee by means of cilia

ex. Balantidium coli
Term
Balantidium coli
Disease-
Source-
Phylum-
Definition
-dysentery
-Feces in drinking water
- Ciliophora
Term
Domain Eukarya; Kingdom Protozoa; Phylum Amoebozoa....
Definition
move and feed by means of Pseudopods (false feet)

ex. Entamoeba histolytica
ex. Balamuthia
Term
Entamoeba histolytica
Disease-
Source-
Phylum-
Definition
-Amoebic Dysentery
-Feces in drinking water
-Amoebozoa
Term
Balamuthia
Disease-
Source-
Phylum-
Definition
-Encephalitis
-Water
-Amoebozoa
Term
Domain Eukarya; Kingdom Protozoa; Phylum Apicomplexa...
Definition
not motile; special organelles penetrate host tissue
-Obligate intracellular parasites
-Complex life cycles (different hosts)

ex. Plasmodium vivax
ex. Cryptosporidium
ex. Toxoplasma gondii
Term
Obligate intracellular parasites
Definition
parasites that can only grow inside living host cells
Term
Plasmodium vivax
Disease-
Source-
Phylum-
Definition
-Malaria
-bite of a female anopheles mosquito
-Apicomplexa
Term
Cryptosporidium
Disease-
Source-
Phylum-
Definition
-Diarrhea
-Humans, animals, H2O
-Apicomplexa
Term
Toxoplasma gondii
Disease-
Source-
Phylum-
Definition
-Toxoplasmosis
-Cats
-Congenital disease passed from mother to fetus and affects blood and lymph vessels
-Apicomplexa
Term
Domain Eukarya; Kingdom Protozoa; Phylum Archaezoa...
Definition
moves by flagella, lives in digestive tract of animals
Term
Trichomonas vaginalis
-Disease
-Source
-Phylum
Definition
-Vaginitis
-Urethra
-Archaezoa
Term
Giardia lamblia (cysts)
-Disease
-Source
-Phylum
Definition
-Diarrhea
-feces in drinking water
-Archaezoa
Term
Domain Eukarya, Kingdome Protozoa, Phylum Euglenazoa
Definition
Moves by flagella; does not sexually reproduce
Term
Tripanosoma brucei gambiense
-Disease
-Source
-Phylum
Definition
-African Sleeping Sickness
-Bite of Tsetse Fly
-Euglenazoa
Term
Algae
-Domain
-Kingdome
Definition
-Prokaryote
-Algae
Term
Algae
-What are their environment?
-Primary producers in what?
Definition
-Most algae live in aquatic or moist environments
- aquatic food chains
Term
Dinoflagellate algae blooms?
-Disease
-Source
-Kingdom
Definition
(Red tide) Produce neurotoxins that cause
-Paralytic Shellfish Poisoning
-contaminated Clams and mussels
-Algae
Term
Phytophthora infestans
-Disease
-Source
-Kingdome
Definition
-Irish Potato Blight (caused the famine in Ireland in 1840)
-Potato
-Algae
Term
Some diverse vegetative structures of Algae?
Definition
-Unicellular ex diatoms and scenedesmus
-Filamentous ex. spiragyra
-colonial ex. volvox
-Multicellular have "body parts"
Term
What are the Multicellular Algae's 5 "body parts"?
Definition
Thallus-body
Holdfasts-roots
stipes-stems
pneumatocytes- used to float
blades- leaves
Term
Algae's Life cycle
Sexually-
Asexually-
Definition
-some reproduce sexually, some alternate generations
-all reproduce asexually
ex. cell division (unicellular)
ex fragmentation (filamentous, multicellular)
Term
Algae Nutrition
Definition
-Photoautotrophic
-Store their food as carbs, starch, glucose polymer and petroleum
Term
Photoautotrophic
Definition
use carbon dioxide as main source of carbon and solar energy to make their own food
Term
Fungi
Definition
-unicellular, ex. yeasts
-multicellular, ex. molds and mushrooms
-eukaryotic
-Chemoheterotrophic
-Reproduce by making spores
Term
Saprophytic
Definition
- feed on dead matter
ex. most fungi
Term
How are fungi grouped
Definition
based on their sexual sprores
Term
Are fungi parasitic?
Definition
Very few of plants and animals
Term
What type of environments do fungi like?
Definition
acidic environments PH5
Term
Mycology
Definition
the study of fungi
Term
Yeasts
Definition
-Unicellular
-Facultative anaerobe
-reproduce asexually
Term
Facultative anaerobe
Definition
Can live with or without O2
Term
3 ways yeasts can reproduce asexually
Definition
Fission
Budding
Pseudohyphae
Term
Buds of yeast are called?
Definition
Blastospores
Term
Blastospore
Definition
is an asexual spore
Term
How do yeasts reproduce sexually?
Definition
by fusing two nuclei and forming ascospores that are enclosed inside an ascus
Term
Ascospores
Definition
Sexual spore inside an ascus
- grouped in Phylum Ascomycota
ex yeasts
Term
Ascus
Definition
Sac that surrounds spores
Term
Molds
Definition
-multicellular fungus
-aerobic
Term
Hyphae
Definition
are long filaments of cells that form a mycelia
Term
Mycelia
Definition
a cottony mass that form from hyphae
Term
Septate hyphae
Definition
have cross-walls that separate cells
Term
Coenocytic hyphae
Definition
don't have cross walls
Term
Vegetateive hyphae
Definition
grow on the surface for molds to absorb nutrients
Term
Aerial hyphae
Definition
grow upward and usually hold reproductive spores
Term
Molds Asexual Spores
Definition
-Sporangiospores
-Conidiospores
Term
Sporangiospore
Definition
are formed inside a sac called a sporangium
-asexual
Term
Conidiospores
Definition
are formed as chains of spores not enclosed
Term
Molds Sexual Spores
Definition
-zygospore
-Ascospores
Term
Zygospore
Definition
-large spore enclosed in a thick wall
Group in Phylum Zygomycota
ex. Plycomyces and Rhizopus
Term
Life Cycle of Fungi
Definition
-Dimorphic
-Teleomorphic
-Anamorphic
Term
Dimorphic fungi
Definition
have 2 forms yeast like or mold like
Term
Teleomorphic fungi
Definition
produce both asexual and sexual spores
Term
Anamorphic fungi
Definition
produce asexual spores
Term
Mycoses
Definition
Fungal Diseases
Term
Systemic
Definition
deep within the body
Term
Cutaneous
Definition
affects skin or hair
Term
Subcutaneous
Definition
beneath the skin
Term
Mycotoxicosis
Definition
illness due to the toxins produced by fungi
ex. Aflatoxin from Aspergillus flavus
Term
Aspergillus Flavus
-what does it do
-where is it found
Fungi
Definition
-Produces aflatoxin and is carcinogenic
-peanuts and grains that are contaminated by this mold
Term
Opportunistic Pathogen
Definition
doesn't ordinarily cause disease but can become pathogenic under certain conditions

ex Canidida albicans
Term
Canidida albicans

Fungus
Definition
can cause a yeast infection
Term
3 Medically Important Phylums of Fungi
Definition
-Zygomycota
-Ascomycota
-Basidiomycota
Term
Domain Eukaryea, Kingdome Fungi, Phylum Zygomycota
Definition
-Zygospores
-Sporangiospores
-Coenocytic
ex. Rhizopus
Term
Domain Eukaryea, Kingdome Fungi, Phylum Ascomycota
Definition
-Ascospores
-Conidiospores
-Septate hyphae
ex. Aspergillus flavus
ex. Candida albicans
ex. Mycosporum
ex. Trichophyton
Term
Mycosporum
Definition
ringworm

under phylum Ascomycota
Term
Trichophyton
Definition
athletes food

under phylum Ascomycota
Term
Domain Eukaryea, Kingdome Fungi, Phylum Basidiomycota
Definition
-Conidiospores
-Septated hyphae
ex. Cryptococcus neoformans
Term
Cryptococcus neoformans

Fungi
Definition
-affects the nervous system (meningitis)

-systematic mycosis
Term
Virus Structure
Definition
-non cellular
-contains only DNA or RNA surrounded by protein coat
-Obligate intracellular parasite
-some have an envelope
-some have spikes
Term
Capsomers
Definition
are the subunits that make up the protein coat that surrounds virus's genetic material
Term
Virion
Definition
Complete virus
Term
Viroid
Definition
Infectious RNA
other parasite that cause disease
Term
Prion
Definition
Infectious protein particle
other parasite that cause disease
Term
Virus Morphology
Definition
Helical-rod
Polyhedral-many sides
Enveloped- spherical, variable shape
Complex virus- T-even (spaces ship)
Term
Taxonomy
-Genus
-Species
-Subspecies
Definition
-______-virus (herpes-virus)
-host (human herpes-virus)
-strain H1N1, H3N2
Term
Bacteriophages
Definition
virus that infects bacteria
Term
2 cycles of Bacteriophages
Definition
Lytic cycle
Lysogenic cycle
Term
Lytic cycle
Definition
phage causes lysis and death of a host cell
Term
5 steps of lytic cycle
Definition
-Attachment
-Penetration
-Biosynthesis
-Maturation (virons are complete)
-Release
Term
Lysogenic cycle
Definition
the prophage is replicated each time the host cell divides
Term
3 steps fo the Lysogenic cycle
Definition
1. Attachment
2. Penetration
3. Prophage
Term
3 outcomes of lysogeny
Definition
Immunity
Phage conversion
Specialized transduction
Term
Immunity form lysogeny
Definition
the lysogenic bacterial host becomes immune to reinfection by the same bacteriophage
Term
Phage Conversion
Definition
the lysogenic host by acquire new genes and traits from the bacteriophage (antibiotic resistant genes can be passed along)
Term
Specialized Transduction
Definition
phage can transfer bacterial genes from one cell to another creating a new strain
Term
Multiplication of Animal Viruses
(seven steps)
Definition
Attachment
Penetration
Endocytosis
Fusion
Uncoating
Provirus- Virus DNA integrates with Host's DNA
Biosynthesis
Maturation
Release by rupture or budding
Term
Latent infection
Definition
remains asymptomatic for long periods
Term
Persistent infections
Definition
process occurs over a long period of time
Term
Oncogenic viruses
Definition
Provirus DNA integrates into host DNA and can caused uncontrolled cell division (tumor)
Term
Identifying Viruses
Definition
Cytopathic effect-compare healthy and infected tissue
Serological Test- use antibodies agglutination
Nucleic Acid Tests- Polymerase Chain Reaction and DNA fingerprinting
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