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Definition
How, when and why Genes are expressed |
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What is the subdivision of the genome? |
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Definition
all genetic material is the Genome and its further divided into Chromosomes and plasmids and then genes and non coding regions |
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what are the 4 nucleotides in DNA |
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Definition
Adenine, Thymine, Cytosine and Guanine |
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what are the 4 nucleotides in RNA |
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Definition
Adenine, Urasil, Cytosine and Guanine |
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What year was the structure of DNA discovered and who got credit? |
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Definition
1953 Francis Crick and James Watson |
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2 enzymes responsible for the uncoiling of DNA |
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Definition
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Enzyme that unzips the unwound DNA |
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Definition
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What is Semi-Conservative replication |
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Definition
1 parental strand + 1 new strand = daughter DNA |
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What is the proofreading enzyme called |
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Definition
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The first step in going from genes to proteins is called (not the entire genome) |
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Definition
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What tells The RNA polymerase to stop |
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Definition
a specific region of DNA known as a terminator |
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Definition
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Definition
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how many non coding codons |
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Definition
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Codon preference or usage bias mainly serves what function |
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Definition
relates to the speed of translation |
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Term
How many t-RNA molecules are there? |
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Definition
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when t-RNA has bound an amino acid what is it called? |
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Definition
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Term
what is the site of the t-RNA called that binds to a codon |
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Definition
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the genetic makeup of an organism |
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Definition
Genotype - Anything an organism can make or do must be encoded for in the genotype |
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the observable or measurable traits an organism has |
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Definition
Phenotype - For example, the ability to ferment lactose is a phenotype |
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Term
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Definition
occur when a change in one nucleotide results in a change of 1 amino acid CTT to CTA |
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Term
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Definition
These mutations occur when a base change results in a nonsense codon, prematurely stopping protein synthesis ATG to ATT (stop) |
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Term
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Definition
Occur when one nucleotide is either added or subtracted from the gene so The message is completely changed |
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Definition
genes passed from parent to offspring |
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Definition
genes passed from one organism to another |
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Definition
refers to the passing of DNA from one bacteria to another in the form of naked DNA |
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Definition
killed mice by mixing non capsulated and dead capsulated bacteria and pulling out live capsulated bacteria. Naked DNA from dead decaying capsulated bacteria was taken up by live bacteria and they learned to make capsules |
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Term
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Definition
are nothing more that small self replicating circular pieces of extra-chromosomal DNA |
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Definition
carry genes for conjugation |
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Definition
carry genes for unusual sugar and carbohydrate usage |
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Definition
carry genes which encode antibiotic resistance |
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Definition
carry genes which encode virulence traits |
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Definition
occurs when a phage (virus of bacteria) accidentally transfers bacterial DNA into another host bacteria |
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Definition
small fragments of DNA which can move around or transpose to different locations in the genome |
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Definition
any protein which decrease or prevent gene transcription This is typically done by impeding the RNA Polymerase |
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Term
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Definition
any protein which promote or increase gene transcription This is typically done by removing repressors or promoting RNA polymerase binding |
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Term
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Definition
Binding site of repressors and inducers located upstream of the coding gene, and typically contains a regulatory gene, a promoter region and an operator region |
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Term
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Definition
is where RNA polymerase binds to the DNA and initiates transcription |
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Definition
is where repressors bind DNA to block transcription |
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Definition
is upstream of both the promoter and operator, and encodes the repressors and inducers |
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Definition
are clusters of several genes which serve one function under control of a single regulatory region |
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Definition
Any use of life (cells, plants, microorganisms) to make a product |
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Term
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Definition
These recognize short sequences of DNA and cut reliable at the same place in that sequence. DNA with blunt or sticky ends |
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Term
Transformation: (introducing a vector) |
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Definition
this is done with chemicals and temperature shock |
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Term
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Definition
this technique uses electricity to make small holes or pores in the cell though which the plasmid can travel. |
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