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microbio
Lab quiz
16
Microbiology
Undergraduate 1
09/25/2014

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Term
Solution
Definition
a homogeneous mixture of two or more substances, which may be solids,liquids,gases,or a combination of these.
Term
Solute
Definition
a substance dissolved in another substance, usually the component of a solution present in the lesser amount
Term
Solvent
Definition
liquid,capable of dissolving another substance
Term
chromophore
Definition
the colored solute in a solution
Term
Stains
Definition
Stains are solutions that contain a solvent and a colored solute called a chromophore
Term
Methylene Blue
Definition
blue, sometimes a blue-green color
Term
Crystal Violet
Definition
blue-purple
Term
Safranin
Definition
purple-red
Term
staining helps determine
Definition
cell morphology, size and arrangement
Term
gram staining uses 2 basic stains
Definition
crystal violet and Safranin
Term
Gram Stain
Definition
purpose to distingguisg between gram positive ans gram- negative bacteria
Term
gram stain steps
Definition
1 flood mear with crystal violet (60 sec)
2 rinse with water
3 flood the smear with iodine (at least 1 min)
4 rinse again with water
5 decolonize with alcohol until fluid runs clear (no longer than 30seconds)
6 rinse again with water
7 counter stain with safranin (60 sec)
8 rinse again with water
9 Blot dry
Term
basic stains have a positively charged chromophore which forms an ionic bond with the negatively charged bacterial cell.
Definition
Term
Gram- positive cells have the ability to resist alcohol decolorization
Definition
gram+cells have lower lipid content in their cell walls.
Term
safranin is used as to counterstain gram- negative cells
Definition
Term
decolotization with ethanol removes crystal violet
Definition
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