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MicroBio Test 3
N/A
65
Biology
Undergraduate 2
03/29/2012

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Term
3 main groups of archaea
Definition
Methanogens (stricly anaerobic, degrade molecules to methane)
Extreme halophiles (obligate aerobes, high salt)
Hyperthermophiles (high temps)
Term
Largest taxonomic group in Bacteria
Definition
Proteobacteria
Term
Streptococci Differentiation most important pathogens
Definition
Beta Hemolytic (completely lyses red blood cells)
Term
How are mycobacteria identified microscopically?
Definition
Acid Fast Test
Term
Study of fungi
Definition
mycology
Term
All fungi are
Definition
chemoheterotrophs
Term
2 parts of molds
Definition
body - hyphae
mass of hyphae - myclelium
Term
Yeasts
Definition
non filamentous unicellular fungi
Term
Lichens
Definition
symbiotic association of fungus and algae
Term
Algae
Definition
simple eukaryotes
photoautotrophic
Term
How much atmospheric oxygen is produced by algae?
Definition
80%
Term
What kind of algae cause red tide?
Definition
genus Alexandrum
Term
Intermediate host of malaria
(where asexual replication occurs)
Definition
humans
Term
Definitive host of malaria
(organism that harbors adult)
Definition
Mosquito
Term
Vector of African Sleeping sickness
Definition
tsetse fly
Term
Vector of Chagas Disease
Definition
kissing bug
Term
Trematodes
Definition
flukes
Term
Cestodes
Definition
tapeworms
Term
Roundworms
Definition
Nematoda
Term
Vector of Epidemic typhus
Definition
lice
Term
Vector of R. M. Spotted Fever
Definition
tick
Term
Vector of bubonic plague
Definition
rat flea
Term
Vector of Lyme Disease
Definition
Deer tick
Term
parts of a Virus
Definition
nucleic acid surrounded by a protein coat
Term
First virus to be seen by electron microscopy
Definition
tobacco mosaic virus
by Wendell Stanley
Term
Protein coat around the nucleic acid core of a virus
Definition
capsid
Term
Who pioneered the method of growing viruses in embryonated eggs?
Definition
Ernest Goodpasture
Term
Describe Plaque Method
Definition
Bacteriophages are mixed with bacteria then put in agar. As the host cells are killed, transparent spots in a cloudy lawn can be seen. Each spot is a plaque and corresponds to one bacteriophage. # of plaques --> plaque forming units
Term
Plaque Method for animal viruses
Definition
cytopathic effect
Term
Steps of Viral Lytic Cycle
Definition
Attachment
Penetration
Biosynthesis
Maturation
Release
Term
When a prophage exits the bacterial chromosome, it can carry and package adjacent bacterial genes
Definition
specialized transduction
Term
How does replication of animal viruses differ from phage replication?
Definition
Penetration (whole virus enters by endocytosis)
Uncoating
Term
Positive strand serves as mRNA for synthesis of capsid proteins
Definition
Positive-sense genome
Term
enveloped positive strand virus
Definition
flaviviridae
Term
caries a viran RNA dependent RNA polymerase that synthesizes the positive RNA strand
Definition
Negative sense RNA genome
Term
One new protein is NA dependent RNA polymerase needed to synthesize negative strands and other new proteins form capsid
Definition
Double stranded RNA genome
Term
RNA genome, but replication occurs by way of a DNA intermediate

carries own RNA dependent DNA polymerase
Definition
Retroviruses
Term
when the double stranded DNA intermediate then permanently integrates into host cell DNA
Definition
provirus
Term
Infectious proteins that catalyze a change in the conformation of normal proteins molecules and cause disease
Definition
prions
Term
Pathology
Definition
scientific study of disease including etiology (cause) and pathogenesis (way it develops)
Term
Infection
Definition
Colonization by a microorganism (doesn't mean you have disease)
Term
Steps in Koch's Postulate
Definition
1. Same pathogen must be present in every case
2. Pathogen must be isolated from diseased host and grown in pure culture
3. Pathogen from pure culture must cause disease when inoculated in healthy animal
4. Pathogen must be isolated from inoculated animal and shown to be the same as original organism
Term
Difference in Sign/Symptom
Definition
Symptom cannot be directly observed (pain discomfort)
Sign is a change in physical appearance (fever, swelling)
Term
Difference between incidence/prevalence of disease
Definition
incidence indicated spread of disease (new cases)
prevalence - old and new
Term
Endemic disease
Definition
always present
Term
Septicemia
Definition
blood poisoning
bacteremia, viremia, toxemia
Term
Development of Disease phases
Definition
Incubation, Prodromal, Period of Illness, Period of Decline, Period of convalescense
Term
Hospital Acquired Infections
Definition
Nosocomial
most common: staph
Term
Father of Epidemiology, studied cholera outbreak
Definition
John Snow
Term
Notifiable Diseases
Definition
AIDS, Measles, Tetanus, Typhoid Fever, Gonorrhea
Term
the ability of a host organism to cause disease by overcoming the defenses of a host
Definition
pathogenicity
Term
Adherence to host tissues
Definition
pathogen has adhesins or ligands
adhesins are usually glycoproteins or lipoproteins
Term
How does capsules aid pathogens
Definition
capsules inhibit phagocytosis by macrophages
Term
M protein of Strep
Definition
Aids in attachment to epithelial cells, resists phagocytosis by making cell think it sees a clot
Term
Waxy lipids of TB
Definition
resists phagocytosis
Term
degrade lysosomal compartment
Definition
leucocidens
Term
hemolysins
Definition
cause disruption of cell membranes of red and white blood cells
Term
How do bacteria gain access to host cell
Definition
membrane ruffling
Term
How do bacterial pathogens damage host cells?
Definition
Direct damage at site of invasion
Use of host resources (iron stolen by siderophores)
Toxin production
Term
Produced by bacteria an released from cell to do damage elsewhere
Definition
Exotoxins: cytotoxins, neurotoxins, enterotoxins
made by gram positive bacteria
Term
A-B toxins
Definition
protein consisting of an active domain and binding domain
ex: diptheria toxin, cholera toxin
Term
Neurotoxins
Definition
interfere with nerve impulse transmission
ex: botulinum toxin, tetanus toxin
Term
Endotoxins
Definition
Produced by gram negative bacteria (in LPS)
lipid portion (Lipid A) is the endotoxin
Term
shock caused by gram negative bacteria
Definition
septic shock
Term
the visible effects of viral infection in a cell
Definition
cytopathic effects (CPE)
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