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MicroBio Test 1
N/A
63
Biology
Undergraduate 2
01/31/2012

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Term
two categories of prokaryotes
Definition
bacteria and archaea
Term
rod like bacteria
Definition
bacillus
Term
spherical shaped bacteria
Definition
coccous
Term
corkscrew/spiral shaped bacteria
Definition
spirillum
Term
fungi cell wall
Definition
chitin
Term
algae cell wall
Definition
cellulose
Term
virus components
Definition
core of nucleic acid and protein
Term
Hippocrates
Definition
person who first observed that disease can be transmitted from person to person and by clothing
Term
Thucydides
Definition
person who first observed that people who had survived and recovered from plague could help sick and not be affected - immunization
Term
Varro
Definition
"invisible animals"
Term
Why does wine sour?
Definition
yeast organisms naturally present in fruit convert sugar to alcohol in presence of air. some bacteria compete for yeast and turn it to vinegar
Term
Robert Koch
Definition
Helped Pasteur develop germ theory of disease. worked with anthrax and animals
Term
Semmelwes
Definition
required doctors to disinfect their hands with chloride of lime
Term
Joseph Lister
Definition
treating surgical wounds and instruments with phenol (carbolic acid)
Term
Alexander Fleming
Definition
discovered penicillin
Term
Jenner
Definition
worked with cow pox, vaccination
Term
What type of microscopy is used to visualize spirochetes?
Definition
darkfield microscopy
Term
what human diseases are caused by spirochetes
Definition
syphilis, lyme disease
Term
confocal microscopy
Definition
specimens stained with fluorochrome antibody and stain internal structures. one plane at a time illluminated with laser. get a 3D image
Term
scanning electron microscope negative staining
Definition
used to see viruses
Term
basic dyes (more common)
Definition
crystal violet, malachite green
Term
acidic dyes
Definition
eosin, picric acid
Term
steps of gram staining
Definition
1. smear of bacteria fixed onto slide
2. slide is covered with crystal violet
3. wash of stain
4. add iodine as mordant
5. wash off (all cells purple)
6. wash with alcohol to decolorize
7. stain with safranin as counter stain
Term
gram positive cells
Definition
purple
Term
gram negative cells
Definition
red
Term
acid fast looks what color
Definition
red
Term
what human diseases are caused by mycobacteria
Definition
tuberculosis, leprosy
Term
steps of acid fast dye
Definition
1. a red dye (carbolfushin) is applied to a fixed smear of bacteria
2. slide is heated
3. slide is treated with alcohol to decolorize
4. slide then counter stained with methylene blue
Term
E. coli of food borne illness
Definition
0157:H7
Term
protein part of enzyme
Definition
apoenzyne
Term
non protein part of enzyme
Definition
cofactor/coenzyme
Term
example of competitive inhibition
Definition
PABA and sulfanilamide
Term
products of glycolysis
Definition
4 ATPs and 2 NADH (net gain 2 ATP)
Term
products of krebs cycle
Definition
8 NADH, 2 FADH, 2 ATP (net gain 2 ATP)
Term
products of electron transport chain
Definition
NADH and FADH are oxidized to yield 34 ATP
Term
where does the electron transport chain occur
Definition
prokaryotes - plasma membrane
eukaryotes - mitochondrial membrane
Term
how does penicillin work
Definition
inhibits final assembly of peptidoglycan in cell wells - leads to weak cells walls - leads to osmotic rupture
Term
characteristics of gram positive bacteria
Definition
thick peptidoglycan layer, sensitive to penicillin, major antigens for serotyping
Term
characteristics of gram negative bacteria
Definition
cel wall includes additional outer membrane, contains LPS, not sensitive to penicillin
Term
what important human pathogens are caused by endospores?
Definition
anthrax, botulism, tetanus
Term
how many naturally occurring elements
Definition
92
Term
how many elements are commonly found in living organisms
Definition
26
Term
acid
Definition
a substance that dissociates into one or more negative ions and one of more hydrogen ions (H+), proton donor
Term
base
Definition
a substance that dissociates into one or more positive ions and one or more hydroxide ions (OH-)
Term
alcohols
Definition
carbon skeleton, covalently bonded hydroxyl group
Term
carbohydrates
Definition
sugars and starches
Term
50% of cells
Definition
proteins
Term
phospholipid
Definition
glycerol, fatty acid, phosphate group
(essential for membranes)
Term
anaerobic respiration
Definition
final electron acceptor is a substance other than oxygen
important for recycling nitrogen and sulfur
Term
steps of ethanol production
Definition
glucose --> pyruvic acid --> acetaldehyde --> ethanol
(2 ATP harvested)
Term
steps of lactic acid production
Definition
glucose --> pyruvic acid --> lactic acid
(2 ATP harvested)
Term
How much energy from fats?
Definition
6 carbon glucose yields 38 ATPs
18 carbon fatty acid yields 147 ATPs
Term
decarboxylation test
Definition
identify bacteria that can catabolize amino acids
color will change to purple
Term
fermentation test
Definition
1. tube that contains a protein, a carb mannitol, a pH indicator, and an inverted tube
2. can't use mannitol --> no color change
3. can convert mannitol to acid --> color change
4. can convert mannitol and produce gas --> color change and gas in the inverted tube (E coli)
Term
light reactions
Definition
cyclic photophosphorylation - electrons return to chlorophyll
noncyclic photophosphorylation - electrons are transferred to NADPH
Term
light independent reactions
Definition
stored electrons are used
1 molecule of glucose takes 6 Calvin cycles
Term
carbohydrate biosynthesis
Definition
ADPG = building blocks for glycogen in bacteria
UDPG = building blocks of glycogen in animals
Term
classification of microorganisms by nutritional pattern
Definition
photosynthesis? yes - phototroph/ no - chemotroph

CO2 as source of carbon? yes - autotroph / no - heterotroph
Term
examples of photoautotrophs
Definition
green sulfur, purple sulfur bacteria, canobacteria
Term
examples of photoheterotrophs
Definition
green and purple nonsulfur bacteria
Term
examples of chemoheterotrophs
Definition
most bacteria, all fungi, protozoa, animals
Term
microorganism that causes bubonic plague
Definition
yersinia pestis
Term
symptoms of bubonic plague
Definition
bubo in groin, swelling in armpit, sudden fever, spitting blood, death
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