Term
HIV 1 and HIV 2 probably came from what animals ? |
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Definition
HIV-1 from chimpanzees HIV-2 from sooty mangabey |
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Term
What subtype of HIV-1 is in USA ? |
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Definition
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Term
What is the first pathogenic human retrovirus ? |
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Definition
Human T-cell leukemia virus |
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Term
What sub-family do HIV-1 and HIV-2 belong in ? |
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Definition
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Term
Reverse transcriptase is what ? |
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Definition
RNA dependent DNA polymerase
Converts ss HIV RNA to ds HIV DNA |
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Term
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Definition
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Term
What enzyme helps HIV insert its DNA into host cell's DNA ? |
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Definition
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Term
What does HIV need to bind CD4 and co-receptors, allowing them to move on and infect other cells ? |
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Definition
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Term
2 reasons HIV is hard to stop ? |
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Definition
1) Reverse transcriptase has low fidelity so can make a lot of errors, and HIV keeps evlovling
2) Heterodimers increase variability of HIV genomes so cell can be co-infected by more than 1 HIV protein |
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Term
HIV enters the body via what cells ? |
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Definition
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Term
HIV encephalopathy is due to ? |
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Definition
Synctial fusion of HIV-infected macrophages and macroglia |
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Term
HIV therapy starts when CD4 + cells fall below ? |
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Definition
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Term
Seroconversion means what ? |
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Definition
When you can detect antibodies |
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Term
What is window period for HIV ? |
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Definition
Negative HIV test (primary infection to seroconversion) Viral load very high, increase high transmission risk |
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Term
Final stage of HIV infection ? |
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Definition
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Term
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Definition
A S L L O
1) Acute infection 2) Strong anti-HIV immune defense 3) Latent resorvior 4) Loss of CD4+ cells and loss of immune response 5) Onset of AIDS |
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Term
What bacteria causes bacterial pneumonia in AIDS pts ? |
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Definition
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Term
AIDS pts can get Mycobacterium Avium Complex under what circumstances ? |
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Definition
Advanced HIV infection and a CD4 lymphocyte count < 50
MAC presents as night sweats, weight loss, abdominal pain, anemia, etc... |
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Term
What is leading cause of death for those living with AIDS ? |
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Definition
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Term
Infections associated with AIDS ? |
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Definition
Bacterial Pneumonia MAC TB Salmoneliosis Bacillary Angiomatosis Viral Hepatitis (Hep B and Hep C) CMV HHV-1, HHV-2 HPV Progressive Multifocal Leukoencephalopathy (PML) - caused by JC virus
A lot of other infections |
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Term
PML is a serious brain infection in AIDS pts. caused by what ? |
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Definition
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Term
Cryptosporidium Parvum becomes self-limiting in individuals with CD4 counts greater than ? |
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Definition
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Term
Most common microorganism that causes infectious pharyngitis ? |
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Definition
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Term
Lower Resp. tract (37 degrees celsius) has no normal flora why ? |
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Definition
It is usually sterile
Particles should be less than 5 microns to get through. Nonciliated epithelium IgG and IgA |
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Term
What droplet tends to remain suspended in the air the longest and can cause infection ? |
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Definition
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Term
Another name for professional invader in a respiratory infection ? |
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Definition
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Term
3 Diseases of lower respiratory tract infections ? |
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Definition
Bronchitis Bronchiolitis Pneumonias |
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Term
Bordet-Gengou growth media can culture what organism ? |
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Definition
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Term
Tinsdale Agar can culture what organism ? |
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Definition
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Term
T or F : Some resp. viruses do not exert their main pathology in resp. tract ? |
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Definition
True
They are transported by respiratory droplets |
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Term
Influenza virus has what type of nucleic acid ? |
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Definition
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Term
Adenoviruses have what type of nucleic acid ? |
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Definition
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Term
Some adenoviral components that interfere with host immune response ? |
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Definition
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Term
Adenovirus Pathogenesis ? |
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Definition
Fiber protein : enables attachment to host cell receptor
Receptor varies with viral serotype
Penton base has toxic activity (inhibition of cellular mRNA synthesis, cell rounding, tissue damage) |
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Term
What virus causes phayngitis and conjunctivitis ? |
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Definition
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Term
Antigenic drift allows rhinoviruses to have ? |
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Definition
High number of viral serotypes |
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Term
What virus can cause common cold and SARS ? |
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Definition
Coronaviruses
ss (+) RNA enveloped helical nucleocapsid have "spike" proteins or peplomers
Transmission mostly via droplets, fecal/oral also possible
Replicate in ** ciliated nasal epithelium ** |
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Term
Etiologic agent for SARS ? |
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Definition
New Coronavirus (SARS-CoV) |
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Term
What viruses cause laryngotrachobronchitis and bronchitis ? |
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Definition
HPIV (Human Parainfluenza viruses)
Family is Paramyxoviridae Divided into 2 sub-famalies
Enveloped Glycoprotein with HN (Hemagglutinin-Neuraminidase) activity
Fusion Factor (F) protein involved in viral entry, and against which antibodies can neutralize virus |
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Term
Which HPIV virus causes Croup (or laryngotrachobronchitis) ? |
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Definition
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Term
Paramyoxovirinae subfamily of viruses evades immune response how ? |
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Definition
Via V proteins (fusion proteins)
Which prevent apoptosis, prevent interferon synthesis, etc... |
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Term
RSV belongs to what virus subfamily ? |
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Definition
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Term
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Definition
1) Entry is via epithelia nose and eye
2) F & G proteins mediate attachment ; F mediates membrane fusion
3) Primary site of replication is nasopharyngeal epithelium Direct CPE is loss of function |
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Term
Do not give what as trt of RSV ? |
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Definition
Heat-killed vaccine, b/c it enhances RSV disease...may due to CD8 T cells (immunopathology) |
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Term
Based on symptoms, how to differentiate b/w cold and flu ? |
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Definition
Flu has fever, headache, vomiting/diarrhea, and severe malaise |
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Term
3 types of influenza virus ? |
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Definition
A (found in birds like ducks, only one classified by HN system, can cause epidemic and pandemic)
B (also in seals, two lineages: Victoria-like and Yamagata-like)
C (no epidemic or pandemic) |
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Term
Influenza / flu is a zoonotic disease ? |
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Definition
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Term
Antigenic Shift and Influenza ? |
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Definition
Is a sudden rearrangement of 8 genetic subunits of influenza virus, usually due to co-infection of two diff. influenza A strains.
Only affects influenza A |
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Term
How do the following Anti-Flu drugs work ? |
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Definition
Amantadine and Rimantadine - Inhibit uncoating of Influenza A only Target M2 protein
Zanamivir and Oseltamvir (Tamiflu) - Inhibit neuraminidase by blocking its release, only works on influenza A and B |
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Term
What does not work against flu / influenza ? |
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Definition
Post-exposure trt
Excessive use of antimicrobials builds up drug-resistance |
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Term
How to distinguish Strep. Pyogenes from all other Beta-hemolytic streptococci ? |
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Definition
Strep. Pyogenes is Bacitracin sensitive |
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Term
How to distinguish Strep. Pneumoniae from all other alpah-hemolytic Strep such as Strep. Viridans ? |
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Definition
Strep. Pneumoniae is optochin sensitive |
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Term
Pneumolysin is one of the virulence factors of what bacteria ? |
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Definition
Strep. Pneumoniae
Is a pore-former inhibits ciliated epithelial activity decreases PMN effectiveness etc... |
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Term
What test is used for determining toxicity of Cornyebacterium Diptheriae ? |
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Definition
Elek test
Lines of precipitin on plate indicates toxigenic |
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Term
How is diptheria exotoxin produced ? |
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Definition
Via lysogenic bacteriophage conversion |
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Term
What bacteria most common cause of epiglottitis ? |
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Definition
Hemophilus influenzae (is a Gram - rod)
**Does not cause influenza** |
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Term
Pathogenesis of H. Influenzae ? |
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Definition
Pili Non-pilus adhesins LPS PRP (Poplyribose Ribitol Phosphate) - antiphagocytic capsule made of this |
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Term
Culture of H. Influenzae requires what ? |
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Definition
Chocolate agar with X factor (hemin) and V factor (NAD) |
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Term
What bacteria causes whooping cough (chronic bronchitis) ? |
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Definition
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Term
Why not use cotton swab or throat swab to culture Bordetella Pertussis ? |
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Definition
B/c the bacteria is very susceptible to drying
But can culture with nasopharyngeal swab or secretions |
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Term
Characteristics of Klebsiella Pneumoniae ? |
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Definition
Non-spore forming Gram - rod (bacillus)
**Necrotizing Pneumonia**
Bronchopneumonia, lung abscesses Two iron uptake systems: aerobactin and enterochelin (are siderophores) "Red currant jelly" sputum |
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Term
Characteristics of Legionella Pneumophila ? |
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Definition
Causes Legionnaire's disease (pneumonia) and Pontiac fever (self-limiting)
Gram - rod, motile, non-spore forming 80% of infections due to serogroup 1
Cannot gram stain b/c bacteria does not take up the counter stain Detect via serology for agglutination or fluorescent antibodies, or with ELISA |
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Term
What bacteria is a Gram - rod, non-hemolytic, ** smells like grapes ** , is found in fresh water and sea water, stains green on media due to pyocyanin and fluorescein pigments, whose most common infection is otitis externa (swimmer's ear), and is dangerous for people who are burn victims or have cystic fibrosis ? |
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Definition
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Term
What two bacteria can cause necrotizing pneumonia ? |
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Definition
Klebsiella Pneumoniae and Pseudomonas |
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Term
Two methods to culture M. tuberculosis ? |
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Definition
Lowenstein-Jensen agar Oleic acid - albumin broth |
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Term
What is a positive result for TB on Tuberculin test ? |
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Definition
Induration > 10 mm diameter |
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Term
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Definition
Prophylactic antimycotics
BCG (Bacillus Calmette-Guerin) vaccine - attenuated M. bovis strain |
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Term
Know TB first line and second line trts
and
TB Drug Resistance categories |
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Definition
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Term
Two clinically significant varieties of Histoplasma Capsulatum (causes Histoplasmosis) ? |
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Definition
H. capsulatum var capsulatum
H. capsulatum var duboisii |
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Term
Most virulent of all human mycotic pathogens ? |
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Definition
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Term
Most common fungal infection seen in AIDS patients ? |
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Definition
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Term
Two forms of Aspergillosis ? |
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Definition
1) Allergic
2) Invasive: hyphae invade tissue
Gan get "fungal ball" (Aspergilloma)
Can get acute pneumonia in severly immunocompromised persons (neutropenia) |
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