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Microbio Exam 1
Microbio 303 Exam 1 Prof. Paustian UW Madison
36
Microbiology
Undergraduate 4
06/25/2010

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Term
Methods of Sterilization
Definition
Autoclaving: Coagulates proteins.
Oven: Coagulates Proteins
Filtration: Filter traps microbes
Radiation: Damages nucleic acids
Gas: Inactivates enzymes
Term
Simple Stain
Definition
Stains Microbe and and differentiates from background.
Term
Differential Stain
Definition
Stains cell differently depending on structure of microbe.
Gram Stain
Term
Specialized Stains
Definition
Stains Structures within a microbe.
Endospore Stain
Term
Building Blocks of Monomers
Definition
Amino acids, lipids, sugars, and nucleotides.
Term
Periplasm Functions
Definition
Hydrolitic Enzymes - Degradation
Binding Proteins and Transport
Chemoreceptors (receptors stim by chems)
Detoxifying enzymes
Osmotic Protection
Term
Cell Wall less forms
Definition
Mycoplasm
Term
Inclusions
Definition
Dense aggregates of particular chemical compounds. Chemical reservoirs.
Term
Surface Structures
Definition
Flagella
Pili and Fimbriae
Glycocalyx
Term
Organelles (E,A,B)
Definition
E- Yes A- No B- No
Term
Mode of uptake (E,A,B)
Definition
E- Endocytosis A + B - Transport by membrane proteins.
Term
Multicellular? (E,A,B)
Definition
E - Yes A + B No
Term
RNA Polymerase (# proteins) (E,A,B)
Definition
E- 12 proteins A- 9-12 B- 4
Term
Ribosome (E,A,B)
Definition
E- 80S, A- 70S, B-70S
Term
Cell walls (E,A,B)
Definition
E- None or Cellulose
A- proteins, pseudomurien, lattice
B- None or Gram (-) or (+)
Term
Lipids (E,A,B)
Definition
E- Ester Linked
A- Ether Linked
B- Ester Linked
Term
Cytoskeleton (E,A,B)
Definition
E- Microfilaments and Microtubules
A + B - FtsZ and MreB
Term
Organelles
Definition
Golgi Apparatus
Lysosomes
Nucleus
Endoplasmic Reticulum
Mitochondria
Plastids
Term
Promoter Structure (E,A,B)
Definition
E + A - TATA Box
B - -10/-35 region
Term
Macronutrients
Definition
Carbon - 50%
Oxygen - 20%
Nitrogen - 14%
Hydrogen - 8%
Phosphorous - 3%
Term
Micronutrients
Definition
Sulfur - 1%
Potassium - 1%
Magnesium - .5%
Calcium - .5%
Iron - .2%
Term
Autotrophs
Definition
Use CO2 as sole carbon
Term
Heterotrophs
Definition
Use reduced carbons
Term
Phototrophs
Definition
Use Light energy
Term
Chemotrophs
Definition
Oxidize Organic and Inorganic compounds
Term
Lithotrophs
Definition
Reduced inorganic molecules
Term
Organotrophs
Definition
extract electrons from organic material
Term
Z values
Definition
Measure Microbial sensitivity to temperature.
Term
Strict Aerobes
Definition
Require oxygen to survive.
Term
Strict Anaerobes
Definition
Unable to use oxygen and are killed by its presence.
Term
Facultative Anaerobes
Definition
Can use (O) if present but can grow in its absence.
Term
Aerotolerant Anaerobes
Definition
Can survive in the presence of Oxygen, but do not use in metabolism.
Term
Microaerophiles
Definition
Require oxygen for their metabolism but cannot live in atmospheric conditions.
Term
Gibbs Free Energy ΔG = ΔH - TΔS
Definition
ΔG = Amount of Free Energy
ΔH = Total Energy
ΔS = Amount of Entropy (more = more chaos)
Term
Oxidation Reduction
Definition
Oxidant + ne- -> Reductant
2H+ + 2e- -> H2
Term
Energy Currency
Definition
ATP
NADH+H
GTP
Phosphoenalpyruvate
Protons
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