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Microbial Metabolism
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Microbiology
Undergraduate 2
10/22/2012

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Term
Catabolic Reactions
Definition
Involve the breaking of larger molecules into smaller molecules(precursor metabolites); Exergonic reaction
Term
Anabolic Reactions
Definition
Synthesize large molecules from smaller products of catabolism; Endergonic reactions
Term
Dehydrogenation Reactions
Definition
Oxidation reaction involving the loss of hydrogen atoms.
Term
Phosphorylation
Definition
Process of storing energy in high-energy phosphate bonds of molecules such as ATP; Inorganic phosphate is added to substrate
Term
Substrate-Level Phosphorylation
Definition
Involves the transfer of phosphate to ADP from another phosphorylated organic compound
Term
Oxidative Phosphorylation
Definition
Energy from redox reaction of respiration is used to attach inorganic phosphate to ADP
Term
Photophosphorylation
Definition
Light energy is used to phosphorylate ADP with inorganic phosphate
Term
Catalysts
Definition
Chemicals that increase the likelihood of a reaction but are not permanently changed in the process
Term
Enzymes
Definition
Organic catalysts
Term
Hydrolases
Definition
Catabolize molecules by adding water in a decomposition process, hydrolization; Primarily used in the depolymerization of macromolecules.
Term
Isomerases
Definition
Rearrange atoms within a molecule but do not add or remove anything (neither catabolic or anabolic)
Term
Ligases/Polymerases
Definition
Join 2 molecules together (anabolic)
Term
Lyases
Definition
Split large molecules without using water (catabolic)
Term
Oxidoreductases
Definition
Remove electrons from or add electrons to various substrates (catabolic and anabolic)
Term
Transferases
Definition
Transfer functional groups, between molecules (anabolic)
Term
Apoenzymes
Definition
Contain protein portions, rather than solely comprised of proteins; Inactive if not bound to nonprotein inorganic ions called Cofactors
Term
Coenzymes
Definition
Either vitamins or ocntain vitamins, which are organic molecules that are required for metabolism but cannot be synthesized by certain organisms
Term
Holoenzyme
Definition
An active enzyme formed by binding an apoenzyme and its cofactor
Term
Ribozymes
Definition
RNA molecules instead of protein molecules; Process other RNA molecules by removing sections of RNA and splicing the remaining pieces together
Term
Hyperthermophiles
Definition
Enzymes that work best above 80 degrees celcius
Term
Denaturation can occur when...
Definition
enzymes lose their 3D shape due to noncovalent bonds breaking
Term
How can pH affect enzyme activity?
Definition
Ions released form acids and bases can interfere with hydrogen bonding and distort an enzyme's 2D/3D structure
Term
As concentration increases...
Definition
enzymatic activity increases
Term
Competitive Inhibitors
Definition
Shaped to fit an enzyme's active site and therefore prevent the substrate from binding; Do not undergo a chemical reaction
Term
Noncompetitive Inhibitors
Definition
Prevent enzymatic activity by binding to an allosteric site located elsewhere on the enzyme; Alters the shape of the active site so the substrate can't bind
Term
Allosteric (Noncompetitive) Inhibition
Definition
Halts enzymatic activity
Term
Excitatory Allosteric Control
Definition
Binds activator molecules to allosteric site, causing a change in the shape which then activates an otherwise inactive enzyme
Term
Feedback Inhibition
Definition
Functions similar to a thermostat controlling a heater; End product is an allosteric inhibitor of an enzyme from an earlier part of the pathway; Slows or stops anabolic pathways when the product is in abundance
Term
Cellular respiration
Definition
Metabolic process involving complete oxidation of substrate molecules and production of ATP by redox reactions
Term
Fermentation
Definition
Results in organic waste products
Term
Glycolysis
Definition
Process that catabolizes 1 molecule of 6-C glucose -> 2 molecules of pyruvic acid and results in a small amount of ATP production
Term
Step 1 Glycolysis- Energy-Investment Stage
Definition
Must invest energy before product can be made; 2 mol ATP invested + 6-C glucose; Those rearrange to make 1,6-biphosphate
Term
Step 2 Glycolysis- Lysis Stage
Definition
Fructose 1,6-biphosphate cleaved into 3-phosphate (G3P)5 and DHAP; Each of these compounds contains 3 carbon atoms freely convertible to the other
Term
Step 3 Glycolysis- Energy-Conserving Stage
Definition
G3P is oxidized into pyruvic acid, giving 2 ATP molecules; DHAP is converted to G3P and also oxidized into pyruvic acid, giving another 2 ATP; Glycolysis also yields 2 molecules of NADH
Term
Substrate-Level Phosphorylation
Definition
The direct transfer of the phosphate between the two substances; In the presence of holoenzyme, the high-energy phosphate in PEP is transferred to an ADP
Term
Decarboxylation
Definition
Removal of carbon dioxide from pyruvic acid yields acetyl Co-A
Term
Synthesis of Acetyl-CoA
Definition
2 pyruvic acid molecules yield 2 molecules of acetyl co-A, 2 molecules of CO2, and 2 molecules of NADH
Term
Kreb's Cycle, TCA cycle, Citric Acid Cycle
Definition
Series of 8 enzymatically catalyzed steps that transfer much of the stored energy to NAD+ and FAD; Occurs in the cytoplasm of prokaryotes and matrix of mitochondria in eukaryotes
Term
Step 1 Kreb's cycle
Definition
Anabolism of citric acid- Splits bond between acetate and coenzyme A which forms 6-carbon compound citric acid
Term
Step 2 Kreb's cycle
Definition
Isomerization reaction
Term
Steps 3-4 Kreb's cycle
Definition
Decarboxylations- Releases 2 molecules of CO2 for each acetyl co-A that enters. For every 2 carbon atoms that enter, 2 are lost to the environment; At this point all 6 carbons have been lost
Term
Step 5 Kreb's cycle
Definition
Substrate level phosphorylation- For every 2 molecules of acetyl Co-A, 2 molecules of ATP are generated
Term
Step 7 Kreb's cycle
Definition
Hydration Reaction
Term
Steps 3-8 Kreb's cycle
Definition
Redox Reaction: Reduce FADH->FADH2; Reduce NAD+->NADH; For every 2 molecules of acetyl Co-A, 6 molecules of NADH and 2 FADH2
Term
Electron Transport
Definition
Series of membrane-bound carrier molecules; Energy from the electrons is used to pump H+ across the membrane, establishing a proton gradient that generates ATP
Term
Flavoproteins
Definition
Carrier molecule; Integral membrane proteins
Term
Ubiquinones
Definition
Carrier molecule; Lipid-soluble, nonprotein carriers
Term
Metal-containing proteins
Definition
Mixed group of integral proteins with a wide ranging number of iron, sulfur, and copper atoms
Term
Cytochromes
Definition
Integral proteins associates with heme
Term
Aerobic Respiration
Definition
Process of the oxygen final electron acceptor binding with H+ to make water
Term
Anaerobic Respiration
Definition
Use molecules instead of oxygen as the final electron acceptor; Critical in recycling nitrogen and sulfur in nature
Term
Chemiosmosis
Definition
Sue of ion gradients to generate ATP by utilizing the flow of ions down their electrochemical gradient across a membrane
Term
What type of phosphorylation happens in chemiosmosis?
Definition
Oxidative phosphorylation because proton gradient is created by the oxidation of components of the ETC
Term
Products of the ETC
Definition
34 molecules of ADP per molecule of glucose; Complete aerobic respiration of a prokayote yields 38 ATP, and eukaryote yields 36 (2 to transport NADH)
Term
Fermentation
Definition
The partial oxidation of sugar to release energy using an organic molecule form within the cell as an electron acceptor; Consists of metabolic reactions to oxidize NADH->NAD+ while reducing endogenous organic molecules which act as final electron acceptors
Term
Main function of fermentation
Definition
Regeneration of NAD+ for glycolysis so that ADP molecules can be phosphorylated into ATP
Term
Photosynthesis
Definition
Capture light energy form the sun and use it to drive the synthesis of carbohydrates from CO2 and water
Term
Chlorophylls
Definition
Molecules of hydrocarbon tail attached to a light-absorbing active site centered around a magnesium ion
Term
Photosystems
Definition
Light harvesting matrices that are embedded in cellular membranes, thylokoids
Term
Prokaryotic thylokoids
Definition
Invaginations of cytoplasmic membranes
Term
Eukaryotic thylokoids
Definition
Infoldings of inner membranes of chloroplasts
Term
Grana
Definition
Stacks of thylokoids in eukaryotic chloroplasts
Term
Stroma
Definition
Space between the outer membrane and the thylakoid membrane
Term
Reaction Center Chlorophyll
Definition
Chlorophyll molecule what all pigments eventually arrive at; When excited the center passes its excited electrons to the electron acceptor (take to ETC)
Term
Cyclic Photophosphorylation
Definition
The final electron acceptor is the original reaction center chlorophyll that donated the electrons
Term
Noncyclic Photophosphorylation
Definition
Requires both PSI and PSII; Generates molecules of ATP and reduces NADP+->NADPH
Term
Light independent reactions
Definition
Do not require light directly; instead use ATP and NADPH generated by the light-reactions
Term
Calvin-Benson Cycle
Definition
Involves attachment of molecules of CO2 to molecules of a 5-carbon organic compound, RuBP; 3 step process
Term
Amphibiolic
Definition
Reactions that can proceed in either direction, catabolism or anabolism
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