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Microbial Growth
Tortora, Funk, Case: Chapter 6 - Study Guide
52
Microbiology
Undergraduate 2
03/20/2011

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Term
Buffer
Definition
Used in media to neutralize acids
Term
Psychrophile
Definition

The general term for organisms capable of growth at

0°C

Term
Thermophile
Definition

Capable of growth at high temperatures;

optimum 50-60°C

Term
Extreme Halophile
Definition
Adapted to high salt concentrations, which are required for growth
Term
Plasmolysis
Definition
A phenomenon that occurs when bacteria are placed in high salt concentrations
Term
Psychrotrophs
Definition
Term used in text for organisms that grow well at refrigerator temperatures; optimum growth is at temperatures of 20-30°C
Term
Capnophile
Definition
Microbes that grow better at high CO2
Term
Hyperthermophile
Definition
Members of the archaea with an optimum growth temperature of 80°C or higher
Term
Hydrogel
Definition
The matrix that makes up a biofilm
Term
Catalase
Definition
An enzyme acting upon hydrogen peroxide
Term
Nitrogen Fixation
Definition
Rhizobium bacteria do this in symbiosis with leguminous plants
Term
Obligate Aerobe
Definition
Requires atmospheric oxygen to grow
Term
Microaerophile
Definition
Requires atmospheric oxygen, but in lower than normal concentrations
Term
Aerotolerant anaerobe
Definition
Does not use oxygen, but grows readily in its presence
Term
Obligate Anaerobe
Definition
Does not use oxygen and usually finds it toxic
Term
Peptones
Definition
Important source of energy, carbon, nitrogen, and sulfur requirements in complex media
Term
Peroxidase
Definition
Breaks down hydrogen peroxide without generation of oxygen
Term
Hydroxyl Radical
Definition
Formed in cytoplasm by ionizing radiation
Term
Catalase
Definition
An enzyme that converts hydrogen peroxide into oxygen and water
Term
Superoxide radicals
Definition
The toxic form of oxygen that is neutralized by superoxide dismutase - synonym for superoxide anions
Term
Oxyrase
Definition
A component added to some culture media that makes the Petri plate into a self-contained anaerobic chamber
Term
Streak Plate
Definition
Isolation method for getting pure cultures; uses an inoculating loop to trace a pattern of inoculum on a solid medium
Term
Spread Plate
Definition
Colonies grow on agar surface for identification
Term
Differential Medium
Definition
Used to increase the numbers of a small minority of microorganisms in a mixed culture to arrive at a detectable level of microorganisms
Term
Lyophilization
Definition
Preservation method that uses quick-freezing and a high vacuum
Term
Colonies
Definition
Accumulations of microbes large enough to see without a microscope
Term
Inoculum
Definition
Microbes added to initiate growth
Term
BSL-3
Definition
Usual laboratory designation for safe handling of tuberculosis
Term
BSL-4
Definition
Laboratory designation for the most dangerous microorganisms; personnel wear "space suits"
Term
BSL-1
Definition
A routine microbiology teaching laboratory would be designated this
Term
Stationary phase
Definition
New cell numbers balanced by death of cells
Term
Lag Phase
Definition
No cell division, but intense metabolic activity
Term
Log Phase
Definition
A logarithmic plot of the population produces an ascending straight line
Term
Reducing Media
Definition
Used to grow obligate anaerobes; generally contain ingredients such as sodium thioglycolate that chemically combine with dissolved oxygen
Term
Selective Media
Definition
Designed to suppress the growth of unwanted bacteria and to encourage growth of desired microbes
Term
Complex Media
Definition
Nutrients are digests or extracts; exact chemical composition varies slightly from batch to batch
Term
Polysaccharide
Definition
Agar is a             derived from a marine alga.
Term
cyanobacteria
Definition

A few bacteria and the photosynthesizing                    

are able to use gaseous nitrogen directly from the atmosphere

Term
Mesophiles
Definition
The most common microbes; their optimum growth temperatures are 25-40°C
Term
Nutrient broth
Definition
A complex medium in liquid form
Term
Absorbance (OD)
Definition
How turbidity is usually recorded in a spectrophotometer
Term
Dry weight measurement
Definition
How the growth of filamentous bacteria is usually recorded
Term
Facultative anaerobes
Definition
Grow more efficiently aerobically than they do anaerobically
Term
Facultative halophiles
Definition
do not require high salt concentrations, but they are able to grow at salt concentrations that may inhibit the growth of many other bacteria.
Term
Phsophate salts, amino acids, and peptones
Definition
are examples of buffers found in complex media
Term
Culture Medium
Definition
Any nutrient material prepared fro the growth of bacteria in a laboratory
Term
Agar
Definition
melts at about the boiling point of water but remains liquid until the temperature drops to about 40°C
Term
Pour plate method
Definition

Dilutions of a bacterial mixture are poured into a Petri dish and mixed with melted agar; this is a plate counting method.

Term
Peptones
Definition
Partially digested protein products that are used in complex media
Term
Living host cells
Definition
Usually necessary to grow obligate intracellular parasites such as rickettsias and chlamydias
Term
Reduction
Definition
The general term for tests that estimate microbial growth by the time required for them to deplete oxygen in the medium is                   tests.
Term
Colony-forming Unit
Definition
When a single colony arises from a clump a bacteria, it is recorded as a(n)                      .
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