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Micro
Genetics
15
Microbiology
Undergraduate 3
05/05/2013

Additional Microbiology Flashcards

 


 

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Term
DNA
Definition
Stores genetic information. Composed of nucleotides (nitrogenous base, sugar, & phosphate). Complimentary, antiparallel, double helix.
Term
Semi-conservative replication
Definition
Results in 2 molecules; each w/ 1 original strand & 1 new strand. Uses multiple enzymes.
Term
DNA helicase
Definition
Unwinds the double helix. Breaks H+ bonds between bases.
Term
DNA polymerase III
Definition
Builds new strands, requires RNA primer.
Term
DNA polymerase I
Definition
Removes & replaces the RNA primers with DNA
Term
DNA ligase
Definition
Joins together DNA fragments.
Term
Transcription
Definition
Process by which RNA is created from a DNA template
Term
Translation
Definition
Process by which a polypeptide strand is created from a mRNA template. Occurs in the ribosome.
Term
Electrophoresis
Definition
Uses an electrical current and a gelatin-like medium to create a visual pattern of DNA fragments.
Term
Oligonucleotides
Definition
Smaller segments of DNA created for research purposes.
Term
Polymerase Chain Reaction (PCR)
Definition
Allows one to create millions of copies of a specific segment of DNA
Term
Conjugation
Definition
Pilus forms a bridge between 2 organisms and a replicated plasmid is transferred.
Term
Transformation
Definition
Cell "picks up" a piece of DNA from the environment usually from a lysed cell. Allows cell to get genetic material from an entirely different species.
Term
Transduction
Definition
Bacteriophage carries genetic material from its previous host cell into the new host.
Term
Transfection
Definition
Artificially introducing genes into organisms. Microbes, plants, or animals.
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