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Micro Vocab 3
Chapter 3 vocab words
37
Microbiology
Undergraduate 2
06/05/2010

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Term
Acid Fast Stain
Definition
differential stain used to identify bacteria that are not decolorized by acid-alcohol
Term
Acidic Dye
Definition
Salt in which the color is in the neg ion
Term
Atomic force microscopy (AFM)
Definition
(scanned probe microscopy) Microscopic tech used to obtain images of molecular shapes characters of chemicals/property to determine temp variations
Term
Basic Dye
Definition
Salt in which the color is in the positive ion (bacterial stains)
Term
Bright field microscope
Definition
Microscope that uses visible light, specimens are viewed against a white background
Term
Capsule
Definition
Outer viscous covering on some bacteria, (polysaccharide & Polypeptide)
Term
Confocal Microscopy
Definition
Light microscope, uses fluorescent stains & laser to make 2 & 3 dimensional shapes
Term
Condenser
Definition
Lens system located below the microscope stage that directs light through the specimen
Term
CMPD Light Microscope (LM)
Definition
instrument w/2 sets of lenses, uses visible light
Term
Counter Stain
Definition
Stain used to give contrast in differntial stain
Term
Darkfield Microscope
Definition
Microscope that has a device to scatter light from the illuminator - specimen appears white on black
Term
Decolorizing agent
Definition
solution used in the process of removing a stain
Term
Differential interference contrast microscope (DIC)
Definition
Instrument that provides a 3D image
Term
Differential Stain
Definition
Stain that distinguishes obj on the basis of reactions to the stain
Term
Electron Microscope
Definition
Microscope that usese electrons instead of light
Term
Endospore
Definition
Resting structure formed inside some bacteria
Term
Fluorescence Microscope
Definition
Microscope that uses a UV light source to illuminate specimens that fluoresce
Term
Fixing
Definition
Attaching a specimen to a slide or in chemicals or (combining elements to enter the food chain)
Term
Fluorescent antibody technique (FA)
Definition
Diagnostic tool using antibodies labeled w/fluorochromes and viewed through a fluorescence scope (immunofluorescence)
Term
Flagellum
Definition
appenage from cell, locomotion(flagellin structure in prokaryote cells and microtubules in eukaryote cells)
Term
Gram stain
Definition
Differential stain that classifies bacteria into 2 groups (g pos/neg)
Term
Gram positive bacteria
Definition
bacteria that retain the crystal violet color after decolorizing by alcohol (turn dk purple)
Term
Gram Neg Bacteria
Definition
Bacteria that lose crystal violet after decolorizing (will stain red with safarinin)
Term
Mordant
Definition
Substance added to stain sol to make it stain intensly
Term
Micrometer
Definition
(mm) equal to 1026 m
Term
Nanometer (nm)
Definition
equal to 1029m or 1023mm
Term
Neg staining
Definition
procedure that results colorless bacteria against a stained background
Term
Ocular lens
Definition
cmpd lm, lens closest to viewer (eyepiece)
Term
Objective lens
Definition
Cmpd lm, lenses close to specimen
Term
Phase-contrast microscope
Definition
Cmpd lm, that allows exam of structures inside cell through specialized condensor
Term
Resolution (resolving pwr)
Definition
Ability to distinguish fine detail with a magnify instrument
Term
Refractive index
Definition
relative velocity w/which light passes through a substance
Term
Scanning Acoustic Microscope (SAM)
Definition
Microscope that uses high-frequency ultrasound waves to penetrate surface
Term
Scanned probe Microscopy
Definition
same as atomic force microscopy (AFM)technique used to obtain images of molecular shapes, char of chem properties, determine temp
Term
Simple stain
Definition
method of staining microorganisms with a single basic dye
Term
Transmission Electron Microscope (TEM)
Definition
Electron microscope that provides high mag (10k-100k)
Term
Total magnification
Definition
magnification of microscopic spec, determined by multiplying the ocular lens by obj lens
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