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Micro unit 3
micro unit 3
104
Biology
12th Grade
05/20/2015

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Term
Oxidative phosphorylation occurs exclusively in aerobic respiration, unlike this type of phosphorylation
Definition
substrate level phosphorylation
Term
Purple and green bacteria reduce H2S->S0->SO42- in these types of environments
Definition
anoxic
Term

true or false: ther terms "Microenvironment" and "Niche" are synonymous

 

Definition
False
Term
this common electron carrier in the electron transport chain contains a heme molecule
Definition
Cytochrome
Term
Non-cyclic electron flow happens exclusively in this process of harnessing light energy
Definition
Oxygenic photosynthesis
Term
This anaerobic process generates a product from SO42- that is toxic to animals and plants
Definition
sulfate reduction (H2S)
Term
Green bacteria have these structures that are especially good at capturing low intensity light
Definition
Chlorosomes
Term
This term describes the biotic and abiotic factors present within a place
Definition
ecosystem
Term
If you had a mutant bacterium that was lacking nitrite oxidoreductase, you would see a build up of what molecule in the environment?
Definition
NO2
Term
The reducing power for many inorganic electron harvesting processes comes from this:
Definition
Reverse electron flow, NADPH
Term
Photosynthesis occurs in these structures in cyanobacteria
Definition
Thylakoid membranes (also found in chloroplasts!)
Term
The nitrogenase enzyme found in nitrogen fixing bacteria is inhibited by this
Definition
Oxygen
Term
If an anaerobic microbe was presented with 2 glucose molecules, and an aerobic microbe was also presented with 2 glucose molecules, what would be the difference in ATP generated between the two? (Assume 1 NADH -> 3 ATP)
Definition
72 ATP
Term
This microbila lifestyle allows for interactions among species, and is found commonly in soil samples
Definition
biofilm formation
Term
Glycolysis begins with glucose, and results in these molecules which go into fermentation or aerobic respiration
Definition
Pyruvate, ATP, NADH
Term
This common electron carrier in the electron transport chain is not a protein and is a source of electrons for reverse electron flow
Definition
Quinones
Term
of the ones we've discussed, these bacteria have the most diverse array of bacteriochlorophylls
Definition
Green Sulfur Bacteria
Term
This is a common source of iron from human activities that leads to acid mine drainage
Definition
Pyrite, FeS2
Term
Microenvironments change quickly due to this, which also contributes to longer generation times of microbes in the wild
Definition
diffusion (of nutrients, molecules)
Term
this term describes the total number of species in a community
Definition
species richness
Term
This term describes the proprtion of species present in a community
Definition
species abundance
Term
These are the light harvesting molecules in anoxygenic photosynthesis
Definition
Bacteriochlorophylls
Term
This process was one of the first to evolve from LUCA
Definition
Methanogenesis
Term
In nitrification, the conversion of NO2- -> NO3- is performed by these organisms
Definition
Nitrobacter
Term
Heterocysts are important structures in this step of the N cycle
Definition
Nitrogen Fixation
Term
This process contributes nitrogen from the soil back into the atmosphere
Definition
Denitrification
Term
True or False: Facultative aerobes will exclusively use aerobic respiration
Definition
False
Term
Mehtanogens are what type of organisms
Definition
Archaea
Term
Methane can be derived from
Definition
CO2, acetate, alcohol
Term
Syntrophy typically occurs in
Definition
Secondary fermentation
Term
What is the most effective e- acceptor in ANaerobic respiration
Definition
NO3-
Term
What is important when considering microbe-microbe interactions for supporting larger scale chemical processes?
Definition
same microenvironment, same habitat, different niche
Term
Biofilm formation can occure where?
Definition
soil and water environments
Term
an important signaling molecules in biofilm formation is
Definition
cyclic-di-GMP
Term
Community
Definition
Collection of 2 or more species (biotic factors)
Term
Population
Definition
all of the organisms of a single species living in a geographical area
Term
Niche:
Definition
The adaptive role of the organism within the system
Term
Soil around plant roots
Definition
Rhizosphere
Term
<1% of microorganisms live here
Definition
Fresh water
Term
2.2% of microorganisms live here
Definition
Marine water: Has much lower nutrient concentrations compared to freshwater
Term
Oligotrophs
Definition
Slow growing, low metabolism, low population density, in marine environments
Term
Prochlorococcus
Definition
  • Photosynthetic bacteria related to cyanobacteria
  • sccount for half of the photsynthetic biomass in tropical and subtropical regions
Term
Advantages of Biofilms
Definition
  • greter acces to nutrients
  • protection from predation and physiochemical distrurbances 
  • stability (less prone to physical disruption)
  • facilitate nutrient nad genetic exchange
Term
Biofilm development
Definition
  1. Attachment: adhesion of a few cells to a suitable solid surface
  2. Colonization: intrcellular communication, growth, and plysaccharide formation
  3. Development: more growth and polysaccharide
Term
Good, bad, and ugly of Biofilms
Definition
  1. Good: Facilitates carbon and nitrogen cycling in the environment
  2. bad: provides means for transmission of pathogens
  3. Ugly: Increased antibiotic resistance which can lead to life threatening infections
Term
The organism responsible for half of the photynthetic biomass in tropical and subtropical regions
Definition
Prochlorococcus
Term
Fermentation as a metabolic strategy requires
Definition
cytoplasmic electron carriers
Term
ATP synthase can:
Definition
  1. use the proton motive force to catalyze the conversion of ATP from ADP
  2. hydrolyze ATP to ADP to pump protons out of the cell
Term
number of ATP molecules consumed in glycolysis:
Definition
2
Term
Total number of ATP molecules generated in glycolysis:
Definition
4
Term
Net number of ATP generated in glycolysis
Definition
2
Term
total number of NADH molecules generated in glycolysis
Definition
2
Term
Net number of ATP generated in glycolysis and oxidative phosphorylation
Definition
8
Term
Number of ATP molecules gnerated in glycolysis + oxidative phosphorylation + the citric acid cycle
Definition
38
Term
Number of ATP molecules generated exclusively in fermentation (not counting glycolysis)
Definition
0
Term
Purple and green bacteria are:
Definition
anoxygenic phototrophs
Term
Process which generates reduced nitrogen to be used for the synthesis of proteins
Definition
nitrogen fixation
Term
process which reduces the amount of fixed nitrogen in the ground
Definition
denitrification
Term
process in the N cycle which uses nitrogen compounds as an electron donor for respiration
Definition
Nitrification
Term
Process in the N cycle which uses organic nitrogen sources
Definition
ammonification
Term
Energetics Balance Sheet for Aerobic Respiration
Definition
[image]
Term

Fermentation e- acceptor is:

Organic vs. Inorganic

Definition
Organic
Term
Alcohol fermentation products
Definition
Reduces pyruvate into ethanol and CO2
Term
Complete process of Fermentation yeilds ____ ATP per 1 glucose
Definition
2
Term

Aerobic repiration e- acceptor:

Organic vs. Inorganic

Definition
Inorganic (usually O2)
Term
Process by which Aerobic respiration produces ATP
Definition
Oxidative phosphorylation (and substrate-level)
Term
the 5 types of electron carriers
Definition
  • (1) NADH dehydrogenases
  • (2) Flavoproteins
  • (3,4) iron-sulfur proteins, cytochromes
  • (5) quinones
Term
NADH dehydrogenases
Definition
[image]
Term
Flavoproteins
Definition
  • Contain a derivative of the vitamin riboflavin (FMN, FAD)
  • The flavin portion is a prosthetic group that is reduced (+ 2 e-, 2H+), then oxidized (-2e-)
Term
Iron-Sulfur proteins, cytochromes
Definition
[image]
Term
Quinones
Definition
  • Hydrophobic molecules (not proteins)
  • move about in the membrane
  • accepts 2 e- and 2 H+ but donates only 2 e-
  • can link between iron-sulfur and cytochromes
Term
how ATP synthase generates ATP
Definition
Proton motive force
Term

Citric Acid Cycle

 

Definition

Feeds into the electron transport chain

Generates:

-GTP

-CO2

-NADH

-FADH2

electrons released feed into the electron transport chain

 

Term
Phototrophy
Definition
the use of light energy
Term
Photosynthesis:
Definition
conversion of light energy to chemical energy
Term
Photoautotrophs vs photoheterotrophs
Definition

Photoautotrophs: use CO2 (most phototrophs are autotrophs)

Photoheterotrophs: use organic carbon

Term
variance in chlorophyll structure allows for what?
Definition
absorption of different wavelengths of light
Term
Different pigments of different organisms
Definition
[image]
Term
Bacterial Pigments and abosrption
Definition
[image]
Term
Where does photosynthesis occur in the cell?
Definition

Photosynthesis components are present in membrane systems

  • Chloroplasts (eukaryotic)
  • Invagination of the cytoplasmic membrane (purple bacteria)
  • from cytoplasmic membrane (heliobacteria)
  • from cytoplasmic membrane + chlorosomes (green bacteria)
  • thylakoid membranes (cyanobacteria)
Term
Arrangement of chlorphylls within the membrane
Definition

In most systems (except in the chlorosome) chlorophylls are attached to proteins in the membrane

[image]

Term
anoxygenic photosynthesis occur where?
Definition

in photosynthetic membranes

  • chromatophores, lamellae
Term
organisms who perform anoxygenic photosynthesis
Definition
  • purple bacteria
  • green sulfur bacteria
  • green nonsulfur bacteria
  • holiobacteria
  • acidobacteria

Note: H2S and H2 are common electron donors

Term
How anoxygenic photosynthesis works
Definition

[image]

 

Cyclic electron flow - > Cyclic photophosphorylation

[image]


 

Term
Photosystem II
Definition
Noncyclic electron flow generates proton motive force
Term
Photosystem I
Definition
Cyclic electron flow when enough NAD(P)H is present
Term
the (sideways) z Scheme:
Definition
[image]
Term
Why is the redox cycle so important in the nitrogen cycle?
Definition

essential for obtaining nitrogen in usable froms

[image]

Term
General nitrogen cycle
Definition
[image]
Term
Nitrogenase:
Definition
  • Inhibited by oxygen
  • rapid removal of O2 by respiration
  • slime layers
  • nitrogenase protect by complexing with another protein = conformational protection
  • special cel types called heterocysts
Term
NH4+-> NO2- performed by
Definition
Nitrosomas
Term
NO2-  ->NO3-performed by
Definition

 

Nitrobacter

 

Term

 

N2 + 8H -> 2NH3 + H2

 

Definition
  • Nitrogen fixation
  • performed by cyanobacteria, purple/green bacteria, others
  • Not performed by any Eukaryotes
  • performed by th enzyme nitrogenase
Term
Where does sulfur come from?
Definition

Rocks, Sediments (CaSO4)

Oceans (SO42-)

Fossil fuel burning (SO2)

 

Term
Significant states of Sulfur
Definition
  • -2 (sulfhydryl, sulfide)
  • 0 (elemental S)
  • +6 (sulfate)
Term
What are the affects of acid rain?
Definition
  • Acidifying soil, damages plants and microbes
  • ocean acidification, damages corals, micro auto- and heterotrophs, others
  • Hydrogen sulfide (H2S) is toxic to many plants and animals
Term
Iron, Nitrogen, Sulfur cycles put together
Definition
[image]
Term
Methanogens are members of what family
Definition

anaerobic Archaea

[image]

Term
Where are methanogens found?
Definition
  • Ruminants (animals with a Rumen: cows)
  • Humans
  • underground 

Note: important contributor to global warming

Term
Key Reactions in methanogenesis
Definition
[image]
Term
Syntrophy
Definition
process whereby two or more organisms cooperate to generate energy through degrading a substance neither can degrade on their own
Term
Fermenter and methanogen syntrophy
Definition
[image]
Term
Anoxic Decomposition Big picture
Definition
[image]
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