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Micro Test 3
Test
120
Biology
Undergraduate 3
04/01/2007

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Term
what MOs would might be found in soft-centered candies?
Definition
yeasts
Clostridium sporogenes
Term
what is 'exploding candy'?
Definition
soft centered candies that have been contaminated with gas producing MOs
Term
explain how invert sugar allow candy industry to fight spoilage?
Definition
1. only so much sucrose can be added until supersaturated
2. Invert sugar contains fructose, glucose and invertase
3. combines glucose and fructose into sucrose, allowing higher sugar concentration to be possible
Term
what does 'commercially sterile' mean in the canning industry?
Definition
target MO and MOs that would grow in the can on the shelf destroyed.
spores definitely present though
Term
what often prevents spores from germinating in canned goods? what may result in germination?
Definition
pH
damage to can
Term
what is the general shelf life of canned foods? what happens after this?
Definition
2 yrs
non-microbial spoilage begins (lower quality, not really dangerous)
Term
what are 2 examples of non-microbial spoilage in canned goods?
Definition
enzymatic browning
corrosion of can
Term
what 3 factors are responsible for microbial spoilage in canned goods?
Definition
inadequate cooling
inadequate heating
damage to can
Term
what is the main type of organism that is most likely to contaminate canned food during the process?
Definition
spore forming thermophiles
Term
what is used to determine the time and temp neede to destroy a target organism?
Definition
inoculated pack study
Term
name MOs that might be used in an inoculated pack study for a canned prodcut? why are these good choices?
Definition
C.thermosacchrolyticum
C.sporogenes
B.stearothermophilus
all non-pathogenic spore-formers with higher heat resistance that C-bot
Term
which of the indicator organisme for canned food is a TA spoiler?
Definition
C.thermosaccharolyticum
Term
which of the indicator organisms for canned foods is a putrefactive spoiler? what strain often used?
Definition
C.sporogenes PA 3679
Term
what indicator organism for canned foods causes flat sour spoilage? what strain often used?
Definition
B.stearothermophilus FS 1518
Term
what are the disadvantages to over-heat treating cans?
Definition
1.texture/color changes
2.vitamin and nutrient breakdown
3.energy cost
Term
what are the three general steps for determining the time and treatment of processing for a new product?
Definition
1. Research literature/existing protocols
2. inoculated pack study
3. plate counts
Term
what percentage of the total organisms are destroyed with each log reduction?
Definition
90%
Term
what is a D-value?
Definition
the time in minutes at a particular temperature (Fahrenheit) that results in 1 log reduction
Term
what is an equivalent kill?
Definition
an equivalent temp/time process that results in the same reduction
Term
what 5 members often make up a food development team?
Definition
1. Food chemist
2. Advertising person
3. Microbiologist
4. Production engineer
5. Nutritionist
Term
what are the steps that would commonly follow if a new food-product idea was accepted?
Definition
prototype
customer survey
in-house sampling
Term
what were the product ideas from the group projects?
Definition
OneShot
Banana butter
Cake in a Box
Maxwell House Plus
Chaloney
PBJ Sticks/ Grilled Cheese Pops
Term
What are the 3 main laws that govern food in the US?
Definition
1906 Pure Food and Drug Act
1938 Food, Drug, and Cosmetic Act
1958 The Delaney Clause
Term
explain the Pure Food and Drug Act
Definition
companies must provide 'safe and wholesome foods', if found unsafe, they can be taken to court
Term
what are the 2 government agencies that are in charge of food in the US?
Definition
the FDA and the USDA (Dept of Ag)
Term
what act pertaining to the USDA came out the same time as the Pure Food and Drug Act?
Definition
the Meat Inspection Act - states that the type of meat must be specified on the label
Term
what event prompted the Food, Drug, and Cosmetic Act?
Definition
increase in manufacturing after WWI
Term
what did the Food, Drug, and Cosmetic Act state?
Definition
1. prohibited manufacturing of unsafe or spoiled food or ingredients
2. holds manufacturers responsible for recalls and drug companies responsible for their drugs
Term
what is the Delaney Clause?
Definition
an ammendum to the Food, Drug, and Cosmetic Act
says that you cannot have known carcinogens in food
Term
what is the GRAS list?
Definition
list of chemicals/ingredients Generally Regarded As Safe
Term
what is one of the best known controversies of the GRAS list? how must this product be labeled?
Definition
saccharin
"has caused cancer in lab rats"
Term
what 3 pieces of infromation are included in a products code #
Definition
location
day
time/shift
Term
what are the two options for recalled foods?
Definition
destruction
re-processing
Term
whare may re-processed recalled foods end up?
Definition
discount stores (dollar store, Amelias, etc.) or overseas markets
Term
what factors are important to developing a sampling plan?
Definition
1.must be random
2.must be representative of the lot
3. must be meticulously documented
4. must involve the complete rejection or acceptance of the lot
Term
what is 'the lot' in regards to quality control?
Definition
all of a product made under the same conditions (same code #)
ex.-shift change, new lot
Term
what determines the jurisdiction of FDA and USDA?
Definition
USDA - unprocessed foods (raw spinach)
FDA - processed foods (frozen spinach)
Term
what is n, C, and m in regards to quality control?
Definition
n - sample size
C - acceptance #
m - value of deficiency
Term
if quality control reports poor quality food products, what can be done?
Definition
1. examine processing
2. increase sample size to get more accurate gauge of product
3. change C value
4. develop a product that can be made from defectives
Term
what is the vendor's risk in quality control?
Definition
probability that the company will hae to throw out an entire lot
Term
what is the consumer's risk?
Definition
the risk that a consumer receives a bad product
Term
which is generally higher, vendor or consumer risk?
Definition
consumer's risk
Term
what is pareve?
Definition
a word found on some products that a rabbi was present during production to assure that the food is kosher
Term
what is HACCP?
Definition
a systematic approach to food safety that addresses prevention.
Hazard Analysis/Critical Control Points
Term
what are the goals of HACCP?
Definition
1. analyze hazards associated with the food
2. determine the critical control points
3. establish control measures for each CCP
4. Establish procedures
5. Develop corrective measures
6. Meticulous record-keeping
Term
what are three types of food hazards and examples?
Definition
1.Biological - pathogens, spoilage
2.Chemical - pesticides, cleaning agents
3.Physical - allergies, foreign objects - glass, hair, plastic, etc
Term
what is a critical control point? what are some examples?
Definition
a point in the process where a hazard may enter
ingredients, processing, storage, shipping
Term
why is record keeping important to HACCP? what types of things are recorded?
Definition
must be a detail paper trail to follow to determine how much product may be hazardous
equipment maintenece, personnel, machinery calibrations, cleaning schedule, etc
Term
what changes are causing an increase in HACCP for the FDA and USDA?
Definition
1.emerging infectious diesases
2.more imported foods
3.more diversity of foods
4.new packaging and processing methods
Term
name two emerging infetious diseases that are affecting HACCP of government agencies?
Definition
Noravirus
E.coli 0157:H7
Term
what is the main cahracteristics of HACCP?
Definition
1.based on solid science
2.preventative, not reactive
3.in depth record-keeping
4.forces manufacturer to be responsible
Term
what agency originally developed HACCP?
Definition
NASA
Term
what are the 4 main types of fermentations?
Definition
1.Lactic acid
2.Yeast
3.Mold
4.Acetic Acid
Term
name a product that involves mold fermentation?
Definition
soy sauce, Roquefort cheese
Term
what are the 3 roles that fermentations have played over history?
Definition
1.Alters flavor
2.Preserves the food
3.Provides a variety of food choices from one source
Term
what does salt do in a vegetable fermentation? how much salt generally added?
Definition
2.5-3.5% w/w NaCl
the salt alters the flavor and pulls nutrient-rich cytoplasm out of cells providing rich substrate for MOs
Term
what does vegetable cytoplasm provide for fermenting organisms?
Definition
vitamins, minerals, glucose from photosynthesis
Term
what are the affects of an anaerobic environement for vegetable fermentations?
Definition
1.inhibits most spoilage MOs
2.allows fermentation (anaerobic process) to occur
Term
what is the pH of most fermented vegetables? why does it stop here?
Definition
3.6
fermentation bacteria inhibited and/or sugar is used up
Term
how are yeasts and mold inhibited one fermnative bacteria are inhibited by pH?
Definition
yeasts and molds are aerobic so product usually packaged anaerobically
Term
what are the 5 main organisms responsible for vegetable fermentations?
Definition
Leuconostoc mesenteroides
Pediococcus sp
S.faecalis
Lactobacillus plantarum
L.brevis
Term
what is the final lactic acid % in sauerkraut?
Definition
1.5%
Term
what are the 3 steps of sauerkraut fermentation?
Definition
1.pH down to 0.6% by Leuconostoc mesenteroides products
2.pH down to 1.2% due to lactic acid of L.plantarum
3.pH down to 1.5-1.7% by L.brevis
Term
what sauerkraut fermenter is homofermentative?
Definition
L.plantarum
Term
what are the products formed by Leuconostoc mesenteroides during the first stage of sauerkraut fermentation?
Definition
lactic acid
acetic acid
mannitol
ethanol
Term
what is the type of relationship between the sauerkraut fermenters?
Definition
symbiotic (commensal)
Term
what are the four types of sausage? which are fermented?
Definition
fresh
smoked
semi-dry (ferm.)
dry (ferm.)
Term
explain the sausage making process
Definition
1.meat chopped up/blended (comminuted meat)
2.packed into casing
3.fermentation
Term
what organisms involved in sausage fermentation?
Definition
Pediococcus w/ Lactobacillus OR
Pediococcus w/Bacillus thuringiensis
Term
what is the substrate of meat fermentations
Definition
glycogen
Term
what are the affects of exercising an animal before slaughter?
Definition
glycogen is decreased (less fermentation)
meat will be tougher
Term
who probably discovered milk fermentations?
Definition
nomadic herders who stored milk in animal stomachs
Term
what is the general succesion of products in milk fermentations?
Definition
Lactose =>
Glucose + Galactose =>
all Glucose =>
Pyruvate =>
Lactic acid
Term
what organisms perform the simplest milk fermentations producing mildly acidic, creamy products.
Definition
S.lactis
S.Cremoris
Term
what products for heterofermenters are used for flavor in milk fermentations?
Definition
CO2
diascetyl
acetic acid
ethanol
endproducts from non-sugar substrates
Term
why is milk a good substrate for fermentations?
Definition
naturally buffered
many different substrates
Term
what organisms involved in Koumiss fermentation?
Definition
S.lactis
Lactobacillus casei
Torula spp.
Term
what do S.lactis and L.casei produce in Koumiss? what does Torula produce?
Definition
bacteria - lactic acid only, makes the curd
yeast - ethanol and CO2
Term
what is the final acidity and alcohol content of Koumiss?
Definition
1% alcohol
1% acidity
Term
what type of milk ususally used in Koumiss?
Definition
mare's milk
Term
where is Koumiss traditionally made?
Definition
W.Asia, Russia, Turkey, N.Iraq
Term
how would you make Koumiss more alcoholic?
Definition
add sugar
add more yeast
add acid to kill lactics before they use up too much substrate
Term
where is Kefir traditionally made?
Definition
W.Russia, Ikraine, Bulgaria, Yugoslavia
Term
what are kefir granules?
Definition
popcorn-like globs that contain bacteria, yeast, and curdled milk
acts as starter for new batches of Kefir
3-D polymers of glucose and galactose formed by L.acidohilus. skimmed and dried from previous batch
Term
what bacteria are often used in making Kefir?
Definition
S.lactis
L.brevis
Leuconostoc kefir
L.acidophilus
Term
what yeasts are often used in kefir?
Definition
Torula
Saccharomyces
Candida
Term
what does L.acidophilus do in the making of Kefir?
Definition
forms 3-D polymers of glucose and galactose that traps MOs and CO2.
Term
what are 2 fairly recent trends in beer?
Definition
more imported beers
more micro-breweries
Term
how old is the oldest known beer recipe?
Definition
6000 yrs old
Term
what are the 4 main phases of beer making?
Definition
malting
mashing
fermentation
post fermentation steps
Term
what are the steps of malting?
Definition
1.barley grains germinated
2.shoots and roots removed
3.remaining parts dried
Term
what is the malt?
Definition
dried barley grains that have been germinated and had roots/shoots removed
Term
why are barley grains allowed to germinate in the malting process?
Definition
1.amylase released from embryo causing breakdown of starch into glucose and maltose
2.proteolytic enzymes breakdown proteins into amino acids
Term
what are the steps of mashing?
Definition
1.malt crushed
2.adjuncts added
3.H2O added
4.held at specific temp for specific time
Term
what time and temp. is mashing often performed at?
Definition
40C <= hour
followed by 70C <= hour
Term
what does the mashing step of beer-making accomplish?
Definition
1.enzymatic changes determining sugar content (affecting favor and alcohol content)
2.surface MO's inhibited by heat treatment
Term
what is the resulting product after mashing?
Definition
wort
Term
what are the steps of the fermentation process in beer making?
Definition
1.Hops added
2.Yeast added
3.Time and temperature determined
Term
what are hops?
Definition
the dried flowers from the Humulus plant
Term
what do hops do for in beer making?
Definition
1.adds to flavor
2.inhibits many MOs
3.contributes to foam by adding proteins
Term
what yeast typically used in beer making?
Definition
Saccharomyces cerevisiae
Term
what is the final range of alcohol content in most beers?
Definition
3.6-5.2%
Term
what is the average time and temp. for beer fermentation?
Definition
8 - 12 days at 3-15C
Term
what are might beer vats be made out or?
Definition
glass
stainless steel
copper or copper lined
Term
how do glass and steel differ from copper beer vats?
Definition
glass/steel - inert materials, no rxtn w/proteins and amino acids - less foam
copper - stabilizes proteins and a.a for better foam production
Term
why do kegs have a much shorter shelf life than cans/bottles?
Definition
cans/bottles are heat treated
kegs are not
Term
what are the post-fermentation steps in beer making?
Definition
1.settling/clarification
2.filtration
3.CO2 adjustment
Term
why is the clarification process performed? what temp?
Definition
to allow yeast and particulate material to settle out of solution
done at 0C
Term
when would filtration be included in a beer making process? what would be used?
Definition
if clear color is desired (most clear bottled beers)
diatomaceous earth used
Term
what does it mean that bacterial death has a 'logarithmic order'?
Definition
decrease in surviving bacter duringa treatment is exponential
survivors plotted on a logarithmic scale results in a straight line
Term
which show lagarithmic order of death - vegetative or spore cells?
Definition
both
Term
what is a survivor curve?
Definition
plot with survivors/volume on y-axis and time on x-ais
Term
what factors in food accect the heat resistance of MOs?
Definition
moisture level
pH
salts, sugars, solutes, colloidal materials
Term
why might two identical products with the same MOs need different heat treatments?
Definition
different initial concentrations of MOs
Term
what is a thermal destruction curve?
Definition
plot of D-values (or TDT values) on logarithmic scale on y-axis vs. temperature on x-axis
Term
what does a TD curve allow you to do?
Definition
determine time and temperatures that will result in the same D-value
determine z-value
Term
what is the z-value?
Definition
the number of degrees F required to reduce the D-value one log cycle (90%)
Term
what is the F-value?
Definition
the TDT at 250F (121C - common autoclave temp)
Term
what may cause deviations from the linear nature of a survivor curve?
Definition
1.spore germination prompted by initial heat
2.clump or chain configurations of bacteria
3.multiple strains of diff. heat resistance
4.microbial injury
Term
what is an example of an MO with a non-linear survivor curve?
Definition
ascospores of Byssochlamys fulva
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