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effects homeostatic activity due to microorganisms infecting body
- first use in reference to bitter wine |
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discovered infectious diseases come from microogranisms |
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Golden Age of Microbiology |
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when most disease causing bacteria were identified and laboratory experiments, asceptic techniques, and microscopes were common |
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Classification of microorganisms |
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all life forms placed into domains based on genetic sequences of rRNA |
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Which domains are prokaryotic? |
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Bacteria and archea
Eukarya is eukaryotic |
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Father of taxonomy (naming) |
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Genus species
- capitalize genus only
-must be underlined or italicized |
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where genetic information is stored for prokaryotes |
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1. Algae
2. Fungi
3. Protozoa
4. Parasite |
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- single celled prokaryotes
- specfic shape/rigid cell wall
- binary fission
- move by flagella
- no nucleus/organelles |
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Definition
1. Bacillus- rod/cylinder
2. Cocci- circular/spherical
3. Spiro- spiral |
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Makes up the cell wall of bacteria ONLY
- also known a murein |
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- comparable to bacteria: no nucleus/ organelles, single celled
- Binary fission, flagella, rigid cell wall
- Grow in extreme environments |
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Definition
Virus- nucleic acid + protein coat
Viroid- nucleic acid (plant disease)
Prion- protein coat (neurodegenerative diseases)
**cannot replicate outside of host** |
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Definition
Lactobacillus
Lactococcus
Streptococcus
Leuconostoc
Pediococcus |
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Definition
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Liquid portion that is removed
nutrient poor |
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added to hasten protein coagulation by converting casein (milk protein, not sugar) |
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Lactobacillus delbrueckii subsp bulgaricus |
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Definition
consumption of exotoxin
Staphylococcus aureus **gram +
Clostridium botulinum |
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Definition
Consumption of living organisms (growth in body)
Salmonella
Campylobacter |
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Hemolytic uretic syndrome
(HUS) |
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Definition
caused byEscherichia coli
fatal, from consumption of rare meat |
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protozoology or parasitology |
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Do microbial organisms cause disease? |
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Definition
Most microbial organisms DO NOT cause disease |
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Large prokaryotic cell exceptions |
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Definition
Thiomargarita namibiensis
Epulopiscium fishelsoni |
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Roles of microorganisms in nature (5) |
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Definition
1. Important members of the ecosystem
2. Food Production
3. Biodegraditive activities
4. Biotechnology
5. Food spoilage/disease |
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Nitrogen in the ecosystem |
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Definition
-Nitrogen cycle depends on bacteria for DNA regulations, amino acids and nucleic acids
-Convert nitrogen to ammonia
-Covert ammonia to nitrates |
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ricotta & cottage cheese
- left at curd stage |
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Steps for cheese production (5) |
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Definition
Inoculation
Coagulation
Whey Removal
Salting
Ripening/maturation |
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Definition
Choose starter culture/lactic acid bacteria
- lowers pH
- denatures proteins |
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Definition
Addition of secondary microorganism
ex) penicillium spp. |
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Possible cheese starter cultures |
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Definition
Lactococcus (streptococcus) lactis Lactococcus (streptococcus) cremoris Lactococcus cremoris subsp. Diacetylactic
Lactobacillus delbrueckii subsp. bulgaricus
+ other lactic acid bacteria |
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Definition
- Commonly from grapes, but can be from anything with sugar
- Ferment sugar to alcohol (ethanol)
- Bacteria used is a yeast- saccharomyces cerevisiae- “brewer’s yeast”
- Collect fluid from crushed grapes, remove everything but liquid and seed
- If skin is left, tannin is added for flavor, results in red or white wine |
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What role does SO2 play in wine production? |
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Definition
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- Combine adjuncts - Add malt- Add mash- Wort forms - Add hops -Boiled/brewed - Add wort in fermentation vat with yeast - Age-Filter/pasteurize- Bottle |
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Definition
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Why can't saccharomyces cerevisae be used in beer production? |
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Definition
Barley is a starch, not a sugar so malt needs to be used |
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What happens when malt is added in beer production? |
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Definition
Germination to activate amylase so starch can be broken down |
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Definition
More adjuncts added to malt
Warm water added to separate solid and form wort (sugar) |
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coagulate enzymes and prevent bad flavor |
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Definition
Cloning
Plants (drought, resistance to insects/molds)
Gene expression
Increased agricultural productivity |
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What is the most prolific producer of antibiotics? |
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Definition
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Types of bacteria that can cause disease |
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Definition
1. Cutaneous
2. Respiratory
3. Gastro-intestinal
4. Neurological
5. Hematological |
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Definition
cause greater harm to microorganism than to the human host
- interfere with essential biological structures or biochemical processes common in microorganisms |
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Prokaryotic cell structure |
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Definition
1. appendages- pili, fimbriae, flagella
2. Cell envelope- glycocalyx, cell wall, cell membrane
3. Cytoplasm- cytosol, ribosomes, granules, nucleoid/chromosomes |
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Cell membrane vs. cell wall |
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Definition
Membrane- a thin membrane (phospholipid bilayer) enclosing the cytoplasm of a cell; proteins in the membrane control passage of ions in and out of the cell
Wall- rigid barrier that surrounds a cell, keeping the contents from bursting out (peptidoglycan in bacteria) |
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Functions of the cell envelope |
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Definition
1. Colonization of environmental surfaces
2. Protection from osmotic imbalance
3. Metabolic functions
4. Protect cell from elimination by endocytosis and infection by viruses
5. Toxic and immunological properties |
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Metabolic functions of cell envelope |
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Definition
1. Location of transport proteins- adhesions
2. Origin of many signal transduction pathways
3. Envelope associated enzyme activities |
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Definition
virus that infects bacteria |
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Definition
Found in all domains, but all organisms do not possess one
- green plants- cellulose
- fungi- chitin
- bacteria- peptidoglycan / murein |
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Functions of the cell wall |
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Definition
1. Protection from osmotic pressure changes
2. Rigidity and stability of cell (interbridges)
3. Morphology and arrangement
4. Basis from gram postive/negative staining reaction |
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Gram positive & negative colors |
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Definition
Positive- blue
Negative- red |
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Definition
Diplococcus, streptococcus, tetrad, cuboidal/sarcinae, staphylococcus |
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Definition
Fusobacteria- sharp edged bacillus
Single, diplobacillus, streptobacillus, cocco-bacillus |
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Spiral, spirochate, vibrio |
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What domain has been shown to contain the majority of disease causing prokaryotic cells? |
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Microorganisms are generally considered to be smaller than... |
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Definition
1 mm
- bacteria are most commonly 1 to 10 micrometers |
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Microorganism responsible for strep throat |
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Definition
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Definition
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Prokaryotic vs. eukaryotic cell wall |
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porkaryotic: peptidoglycan, muerin, tecihoic acid
eukaryotic: plants=cellulose, fungi=chitin
similar: made up of monomers of sugars (amino sugars) |
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Definition
group of microogranisms classified based on antigens |
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Definition
group of microorganisms classified based on shape |
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0157, 0166 --> have different antigens on surface
-0157 is toxic to bacterial envelope |
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1mm- human
micrometer- microscope
nanometer- electron microscope
.1 nm/1 angstrom- special microscope |
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