Shared Flashcard Set

Details

Micro midterm
Just studying
48
Microbiology
Undergraduate 2
02/28/2014

Additional Microbiology Flashcards

 


 

Cards

Term
Steps of translation
Definition
Initiation
Elongation
Termination
Protein folding
Protein processing
Term
Codon
Definition
3base sequence of mRNA that codes for a specific amino acid
Term
Enzymes involved in replication
Definition
Helicase (untwisting, breaking h-bonds)
Primase (synthesizing RNA primer)
DNA Polymerase 2 (adds bases, proofreads)
DNA Polymerase 1 (remove RNA primer, close gaps, repair mismatches)
Ligase (final binding of nicks in DNA synthesis and repair)
Toiposomerases (supercoiling and untangling)
Term
Inducible operon
Definition
Is usually in off mode, until the appropriate substrate binds to its allosteric site
Term
Repressive operon
Definition
Normally in on mode, and will be turned off when the nutrientis no longer required; excess nutrient serves as corepressor needed to block the action of the operon
Term
Spontaneous Mutations
Definition
Random changes in DNA due to replication error
Term
Induced mutations
Definition
Exposed to mutagens in environment
Term
3 means for recombination
Definition
Conjugation, transformation, and transduction
Term
Conjugation
Definition
Sex pilus of F+ bacteria (contains plasmid) transfers plasmid into F- bacteria
Viable factors: virulence, resistance genes
Term
Transformation
Definition
Free floating DNA, cell must be competent (have proper membrane receptors to pick up DNA)
Unlimited cloning techniques (dead cells can give to live cells
Term
Transduction
Definition
Generalized: random fragments of host DNA are taken up by the phage during assembly
Specialized: a highly specific part of the host genome is regularly incorporated into the virus
Term
Sterilization
Definition
Destruction of ALL microbial life
Term
Disinfection
Definition
Destroys most microbial life, reducing contamination on inanimate surfaces
Term
Asepsis
Definition
Destroys most microbial life on living surfaces (iodine)
Term
Decontamination
Definition
The mechanical removal of most microbes from a living or non-living surface (hand washing, filtration)
Term
Bacteristatic
Definition
Agents that prevent the growth of bacteria on tissues or objects
Term
Bactericidal
Definition
Agent that destroys all bacteria except for those in the endospore stage
Term
Anti-microbial targets:cell membrane
Definition
Detergents (surfactants) work as microbial agents, break open phospholipid bilayer
Term
Anti-microbial targets: cell wall
Definition
chem, detergents, alcohol) block synthesis, digest it, breakdown surface
Term
Nucleotide
Definition
Phosphate, sugar, nitrogenous base
Term
Purpose of ETC
Definition
Form ATP from ATP Synthase
Term
Fermentation
Definition
Incomplete oxidation of glucose or other carbohydrates in the absence of oxygen
Term
Anaerobic respiration
Definition
Utilizes oxygen containing ions instead of free oxygen (CO3, H2O...)
Term
Exoenzyme
Definition
Transported extracellularly where they breakdown large food molecules or harmful chemicals
Term
Endoenzyme
Definition
Break things down in cell
Term
Denatured
Definition
Weak bonds that maintain native shape of apoenzyme are broken
Term
Levels of structure
Definition
Primary:polypeptide chain
Secondary: intrachain bonds and folds (helical or sheets)
Tertiary: 3D folding
Quaternary: several 3d shapes bound together by weak forces
Term
Co-factor
Definition
Non-protein molecules (organic, inorganic molecules) that help enzymatic reactions
Term
Synergism
Definition
Members cooperate and share nutrients
Term
Antagonism
Definition
Some members are inhibited or destroyed by others (antibiotics)
Term
Mutualism
Definition
Obligatory dependent; both members benefit
Term
Commensalism
Definition
One benefits; other member not harmed
Term
Parasitism
Definition
Parasite dependent; host harmed
Term
Capnophile
Definition
Grows best at higher co2 tension than is normally present in atmosphere (streptococcus pneumonia)
Term
Quorum sensing
Definition
Monitor population growth and interact with each other (share info, harder to kill).
Term
Nf=(Ni) 2^n
Definition
Total number of cells= (starting number of cells) 2^ total time/generation time
Term
Superoxide dismutase
Definition
2o2 +2h ----> h2o2 +o2
Term
Catalase
Definition
2h2o2 -------> 2h2o + o2
Term
Psychrophile
Definition
-15degrees ---> 15degrees freezer microbes
Term
Mesophile
Definition
Human pathogens; 20-40 degrees Celsius (10-50 in extremes)
Term
Thermopile
Definition
Optimally >45 degrees; 45-80
Term
Extremophile
Definition
80-121 (Cyanobacteria)
Term
2 categories of microbe nutrition
Definition
Macronutrients (CHO)
Micronutrients (trace elements)
Term
Lithotroph
Definition
Relies totally on inorganic molecules (micronutrients)
Term
Phototroph
Definition
Uses light rays and transforms them into chemical energy
Term
Chemotroph
Definition
Use organic compounds for energy and inorganic compounds as a carbon source
Term
Environmental factors on microbes
Definition
Gases
Ph
Temperature
Ecological associations
Osmotic pressure
Term
Total ATP outcomes:aerobic, anaerobic, fermentation
Definition
Aerobic: 34-38
Anaerobic: 2-36
Fermentation: 2
Supporting users have an ad free experience!