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Micro Lecture Exam 2
11/07/12
89
Biology
Undergraduate 4
11/06/2012

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Term
1. Caulobacter
Definition
GRAM NEGATIVE

a. Asymmetrical binary division
b. Starts off as a vibrio form
c. Prostecate (appendaged), no flagella
i. Increase SA to absorb nutrients (lazy organism)
ii. Reproductionbudding
iii. Attachment via holdfast
Term
a. Borrelia burgdorferi
Definition
GRAM NEGATIVE
i. SPIROCHETE; Anaerobic/microaerophillic
ii. Lyme disease; tick vector
iii. ECM: Erythena chronicum migran
iv. Ixodes pacificus (disease)
Term
b. Borrelia recurrentis
Definition
GRAM NEGATIVE SPIROCHETE
i. Relapsing fever; tick vector
ii. Organism keeps producing new sets of antigen
Term
c. Treponema pallidum
Definition
GRAM NEGATIVE SPIROCHETE
i. Anaerobe
ii. Syphilis + Yaws
iii. Active systemic.
Term
a. Myxococcus xanthus
Definition
GRAM NEGATIVE

i. Unicellular glider
ii. Myxocyst with myxospore
iii. Forms fruiting bodies.
Term
b. Beggiatoa
Definition
GRAM NEGATIVE

i. Filamentous or trichome
ii. Sulfur granules
Term
4. Spirillum (3 organisms)
Definition
a. Rhodospirillum (+)
b. Spirilla
c. Helicobacter pylori
i. Cause gastic ulcers
Term
a. Desulfovibrio
Definition
GRAM NEGATIVE

Vibrio
i. Anaerobic respire
Term
b. Bdellovibrio
Definition
GRAM NEGATIVE
Vibrio
i. Parasite of bacteria
ii. Grows/replicate DNA inside host bacteria
Term
c. Vibrio cholera
Definition
GRAM NEGATIVE

i. Causes cholera (dehydrates people)
ii. Epidemic INTOXICATION
iii. Fecal Oral transmission
iv. Self-limiting
v. Use oral dehydration system
Term
6. Pseudomonas aeruginosa
Definition
GRAM NEGATIVE

a. ONE polar flagella
b. Opportunistic
c. Use oil as nutrient carbon source
Term
7. Salmonella typhosa
Definition
a. Peritrichous flagella
b. Cause typhoid fever
Term
8. Actinomyces
Definition
Forms fungus-like branched networks of hyphae

4 Examples
Corynebacterium
Streptomyces
Mycobacteria
Actinomyces
Term
9. Streptomyces
Definition
a. Geomins (soil smell)
b. Produces tetracycline (antibiotic)
c. Hyphae
d. Spores
Term
10. Corynebacterium diphtheriae
Definition
a. Club-shaped (Coryneform)
b. Metachromatic granules (phosphate storage)
Term
11. Acetic acid bacteria
Definition
GRAM NEGATIVE

a. Actobacter sp.
i. Aerobic, motile
ii. Incomplete oxidation of ethanol
Term
12. Nitrogen-fixing bacteria
Definition
GRAM NEGATIVE

a. Klebsiella
b. Azotobacter
c. Rhizobium
Term
16. Staphylococcus
Definition
GP
falcutative
catalse positive
salt tolerant
pH tolerant
Term
17. Micrococcus
Definition
a. Sarcina or tetrad
Term
20. Rickettsia sp.
Definition
a. Tick vector
b. Rocky Mountain fever
c. Reticulate bodies in tick nymph
d. Microbe phagocytized into cell and lysed
e. F actin
Term
21. Chlamydia
Definition
a. Cell wall but NO PEPTIDOGLYCAN
b. Elementary body: infective, rigid cell wall
c. Reticulate body: active, flexible cell wall
Term
E.coli
Definition
GI tract of warm blooded animals
enterotoxin/enteroinvasive

Motile
Ferments Glucose + lactose
NEGATIVE for citrase, H2S, urease, and VP
Term
Shigella sp.
Definition
NON-motile
Ferments glucose but not lactose
NEGATIVE for citrase, H2S, urease, and VP

Causes amoebic dysentery and produce neurotoxin. Infects captive population with poor sanitation.
Term
Salmonella sp.
Definition
Motile
Ferments glucose but not lactose
POSITIVE for citrase and H2S
NEGATIVE for urease and VP
Term
Salmonella typhi
Definition
1 serotype
Enteric typhoid fever
Term
Salmonella cholerasuis
Definition
1 serotype
Septiemias
Less severe than typhoid
Term
Salmonella enteridis
Definition
1300 serotypes by O, K, and H antigens
O: somatic, based on sugar linkage in O-LPS link
K: capsule of bactera
H: flagella of bacteria

Causes food poisoning
Term
Proteus sp.
Definition
rapid motility (swarms)

Motile
Ferments glucose but not lactuse
POSITIVE for urease and H2S
NEGATIVE for VP
Term
Enteric, butanedoil fermentors (VP POSITIVE)
Definition
Klebsiella sp
Enterobacter sp.
Serratia sp. (bright red pigment)
Term
Yersinia pestis
Definition
Bubonic + pneumonic plague vector
Bubo = lymph nodes, which gets into human lungs
Pneumonic: fluids cause human to cough and transfer to others.
Term
Legionella sp
Definition
legionaire's disease
PONTIAC FEVER
Term
Brucella sp.
Definition
brucellosis in MILK
pasteurize to get rid of it, or eradicate by killing cow
Term
Bordetella sp.
Definition
whooping cough
DPT vaccine; P is for pertuscous, which is cause of whooping cough
Term
Neisseria gonorrhoeae
Definition
SRD gonorrhea
Inflammation of neutrophils
Term
Neisseria meningitidis
Definition
Inflammation of CNS
Term
Sporosarcina
Definition
GP/endospore
cocci
sarcinal
strict respirer
Term
Bacillus polymyxin
Definition
GP/endospore
makes antibiotics (polyxin and bacitracins that makes it thrive in soil
Term
Bacillus thiringensis
Definition
GP/endospore
pesticidal
Term
Bacillus cereus
Definition
GP/endospore
food poisoning via enterotoxin
gastroidis poisoning
Term
Bacillus anthracis
Definition
GP/endospore
causes anthrax
Term
Clostridium sp
Definition
GP/endospore
peritrichous flagella
Habitat: soil and gut of warm blooded animals
Term
Clostridium botulinum
Definition
GP/endospore

botulism via neurotoxin
cause respiratory failuer-->death
Term
Clostridium perfringens
Definition
GP/endospore

several toxins (hemolytic, necrotizing, enterotoxin)
gangrene disease; food poisoning
also found in turkey!
Term
Clostridium tetani
Definition
GP/endospore
Causes tetanus
DPT: T stands for tetanus
spores from soil get on nail and toxin gets into body.

Causes people to HYPERFLEX
Term
DPT
Definition
D: diphtheria
P: pertuscous (whooping cough)
T: tetanus
Term
ENDOSPORE GRAM POSITIVE(list oxygen requirements)
Definition
Aerobic/facultative: cocci and bacilli
Anaerobic: Clostridium
Term
Staphylococcus sp.
Definition
Falcutative
CATALASE POSITIVE
Salt tolerant
Term
Catalase positive test implies:
Definition
GP
Aerobic respiration
Electron transport chain, H2O2, and O2
Term
Pasteurella sp.
Definition
animal pathogen
GN bacilli
Term
Francisella tulerensis
Definition
tuleremia
GN bacilli
Term
Staphylocccus aureus
Definition
GP cocci
pathogenic
hemolysin
penicillinase
exotoxin
protease
Term
Staph. epidermis
Definition
GP cocci
ferments glucose but not mannitol
Term
Micrococcus luteus
Definition
obligate aerobe, chemoheterotroph, non pathogenic
Term
Lactic acid
Definition
GP
lactic acid by fermentation

Cocci makes homofermenters and heterofermenters
Term
Lactobacillus
Definition
Term
Lactobacillus
+details
Definition
GP rod
lactic acid
catalase negative

Prevents yeast infection in women
grows on mucosal membrane and produce acid to make vagina inhospitable for yeast.

Antibiotics kills lactobacillus though; makes vagina susceptible to yeast infection.
Term
Lactic acid COCCUS homofermentors
Definition
Streptococcus (streptococcal arrangment)
Pediococcus (tetrad arrangement)
Term
Streptococcus pygoenes (characteristics AND SUCCESSION OF MICROBIAL COLONIZATION details)
Definition
GP lactic acid bactera

CW: ABCFG
Hemolysin: beta
Group: pyogenes
Causes strep throat, skin lesion, pneumatic fever

M PROTEIN: resembles heart tissue. autoimmune phenomenon cause antibody to attack both bacteria and heart tissue-->BAD
Term
Streptococcus mutans (characteristics AND details)
Definition
GP lactic acid bactera

CW: not gpd
Hemolysin: alpha
Group: viridans
Dental cavities

SUCCESSION OF MICROBIAL COLONIZATION
S. salivaries colonizes on tooth. Actinomyces viscosis comes in. then S mutans attaches to and covers actinomyces. Ferments glucose and fructose.
Term
Streptococcus fecalis
Definition
GP lactic acid bactera

CW: D
Hemolysin: alpha, beta
Group: enterococcus
GI tract (indicator organism)
Term
Streptococcus cremoris
Definition
Term
Streptococcus pneumococcus
Definition
GP lactic acid bactera

Causes pneumonia
Term
Streptococcus lactis
Definition
GP lactic acid
ferments milk; reduce pH; precipitates milk protein casein. Creates whey (liquid) and precipitated casien (curd)
Term
Lactobacillus casei
Definition
2NDARY FERMENTATION

After strep. lactis precipitates casein to curd, l.casei uses it to make jack cheese and cheddar.
Term
Proprionbacterium shermanii
Definition
A part of 2ndary fermentation for SWISS CHEESE

A part of the coryneform bacteria: GP, club shaped

Has 3 products
1. acetic acid: gives cheese sharp taste
2. propianic acid: gives nutty flavor
3. CO2: holes
Term
Proprionbacterium camemberti
Definition
2NDARY FERMENTATIOn
Makes camembert cheese
Term
Actinomycete Line (4 organisms)
Definition
1. Coryneform (diphtheroid) bacteria: club shaped
2. Mycobacteria: clubbed with semi branches
3. Actinomyces: true branches
4. Streptomyces: true branches with spores
Term
Coryneform bacteria (details)
Definition
Nonmotile, rod, GP, pallisade form common
Term
Corynebacteria diphtheriae
Definition
GP coryneform
NASTY organism
D: diphterium; affects upper respiratory tract
Term
Corynebacteria diphtheriae
Definition
D for diphetrium
affects respiratory tract
Term
Mycobacterium sp. (Plus name 3 of the species)
Definition
mycolic acid
hydrophobic, doesn't want to spread out in agar
pleomorphic

1. M. tuberculosis
2. M. leprae (nervous tissue)
3. M. avium (AIDS)
Term
Actinomyces sp.
Definition
mycelial/branched colony growth. Found in nasopharynx (nasal area) and SOIL
Term
Streptomyces sp.
Definition
GP
filamentous, branched w/ spores
makes antibiotics and enzymes
SOIL SMELL
Term
Chlamydias
Definition
GN

Obligate intracellular ATP parasite
DNA and RNA, but no ATP

Infect mucosa
2 forms: aerosal or sexual transmission

Elementary body (EB) is infective stage, which is taken in and neutralizes lysosomes and reticulate body (RB) spread into blood
Term
Chlamydias psitticosis
Definition
GN
causes psitticosis

found in exotic birds--> transmitted to humans

EB --> RB
Term
Chlamydias trachomatis
Definition
GN

Causes conjunctivitis/urethrisits/cerivitis

sexually transmitted
asymptomatic

EB --> RB
Term
Rickettsias sp.
Definition
GN
intracellular parasite
vector borne by ticks/fleas
cytochrome system (makes its own ATP)
growth factors parasite

Ticks transfer EB to humans
Term
Rickettsias prowezekii
Definition
typhus fever
epidemic fever
Term
Rickettsias rickettsii
Definition
rocky mountain SPOTTED FEVER
Term
Mycoplasma sp.
Definition
no cell wall
cytochrome system to respire/ferment
Need serum to live
not intracellular parasite
osmotically stable
Term
Coxiella brunetti
Definition
Related organism to Rickettsias sp.
Target organism for milk pasteurization
Term
Acholeplasma sp.
Definition
Mycoplasma

NO cholestorol requirement (sterol)
falcutative
Term
Mycoplasma pneumoniae
Definition
Atypical pneumonia in humans

Penicillin can't kill it because it doesn't have cell wall. Use other antibiotics that inhibit protein synthesis to kill this bacteria
Term
Mycoplasma mycoides
Definition
bovine contagious pleupneumonia
Term
Spiroplasma
Definition
Mycoplasma family

vector borne disease of TREES
Term
Ureaplasm
Definition
Mycoplasma family

grows in urinary tract. Not associated with diseases.
Term
Archaebacteria's cell wall and cell membrane
Definition
CW:
1. no peptidoglycan (muramic acid)
2. S layer (paracrystaline surface layer)
3. thermoplasma have no CW

CM:
1. phosphoglycerol with "R" groups linked via ether links
2. NO FATTY ACIDS; Isoprene instead
3. Glycerol di-ethers and tetra-ethers
Term
Extreme halophiles (Archae)
Definition
Carotenoid pigments protects from UV light

Bacteriorhodopsin harvests light and produces ATP
Term
Thermus aquatics
Definition
TAC polymerase

Its DNA polymerase was RESISTANT to heat. Today, it's used in PCR.

Heat loving organism found in Yellow Stone
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