Shared Flashcard Set

Details

Micro lab rules
lab rules
75
Microbiology
Undergraduate 1
05/17/2011

Additional Microbiology Flashcards

 


 

Cards

Term
3. Upon entering the lab each day, the following tasks must be performed in this order:
Definition
a. Wash hands
b. Don lab coat
c. Put on gloves
d. Disinfect the bench
Term
leaving the lab perform what activities in reverse order
Definition
disinfect bench tops
take off gloves
take off coat
wash hands
Term
a small glass tube that liquid is drawn into so that it can be measured, often b4 it is delivered to another container
Definition
pipette
Term
Never place any instruments or materials into your mouth. (i.e. Do not pipette by
mouth. Use the
Definition
mechanical apparatus provided
Term
. Cultures must always be carried in a __________ when moving around the laboratory
Definition
test tube rack
Term
b. Cultures must be kept in a _____________ on the bench tops when not in use.
Definition
test tube rack
Term
Broth cultures must never be __________ by mouth. Always use a suction aid (never use your mouth) when filling a pipette or use a pipettor with a biological or chemical reagent
Definition
pipetted
Term
Broken glass, used slides, pipettes, and swabs should all be disposed of in the
Definition
sharps container
Term
Gloves should be disposed of in the ___________. Gloves NEVER go in the
normal trash cans.
Definition
used gloves receptacle
Term
Label all materials with
Definition
name, date and the organism that is being cultivated.
Term
To avoid contamination, all instruments such as loops and needles should be sterilized by ______

. Nothing used for the transfer of bacteria should ever touch
the bench tops.
Definition
incineration
Term
Sterilization techniques will involve the use of __________ that are fire and burn hazards. _________ reach an internal temperature of 850 ºC (1500 ºF). Keep all combustibles away from the _______.
a. Do not leave inoculating loops or needles propped in the ___________.
Take caution with loops and needles. They should never be waved to cool
Definition
Bacticinerators
Term
7. Proper procedure for transferring cultures should always be followed:

_______ loops/needles before use.
Definition
sterilize
Term
7. Proper procedure for transferring cultures should always be followed:

. properly remove the caps to tubes- so the rim is not touching your
Definition
glove
Term
7. Proper procedure for transferring cultures should always be followed:

Never place caps on the
Definition
bench tops
Term
7. Proper procedure for transferring cultures should always be followed:
Only hold ______ tube at a time
Definition
one
Term
7. Proper procedure for transferring cultures should always be followed:

Bring the liquid to
Definition
you
Term
7. Proper procedure for transferring cultures should always be followed:

Only insert the portion of the ___________ that got red hot in the
Bacticinerator (this is the only portion of the _______ that is sterile)
Definition
loope nor needle
Term
7. Proper procedure for transferring cultures should always be followed:

Replace the cap and put the tube back in the ________
Definition
rack
Term
7. Proper procedure for transferring cultures should always be followed:
_______ the loop/needle before returning it to its container.
Definition
re-stirilize
Term
. Observe _______ technique at al times when dealing with microbial cultures
Definition
aseptic
Term
aseptic
Definition
free from the living germs of disease, fermentation, or putrefaction
Term
10. Place all cultures in the appropriate __________ or in designated waste areas.
Definition
incubators
Term
You will be responsible for the proper care of any microscope that you are using.
Make sure that you _______ each microscope that you get and that you inform the instructor of any issues with the microscope.
Definition
sign out
Term
All microscopes must be _______ before and after use.
Definition
cleaned
Term
Oil immersion should only be used with the ____________
always rotate the objectives in a clock-wise fashion to avoid exposing other objectives to the oil.
Definition
100x oil immersion objective
Term
5. Procedure For Cleaning Microscopes:

Move the stage to the ______________. Use the ______ adjustment knob to obtain maximum working distance and remove the slide from the stage.
Definition
lowest position

coarse
Term
5. Procedure For Cleaning Microscopes:

turn off th __ and _____________ the cord. store _ appropiately.
Definition
light

cord
Term
5. Procedure For Cleaning Microscopes:

Using lens paper, clean all the lenses starting with the cleanest first ___ ____ ______ and ______. use lens cleaner if necessary
Definition
ocular, low power, high power and oil immersion.
Term
5. Procedure For Cleaning Microscopes:
Clean any oil off of the stage using
Definition
Kimwipes
Term
5. Procedure For Cleaning Microscopes:
rotate the __________ into place.
Definition
scanning objective (4x_
Term
label culture vessels w/ non-water soluable gass marckers and or self stick labels prior to
Definition
inuculation
Term
place the lABELING DIRECTLY BELOW THE ___________ of the culture tube
Definition
cap
Term
when labeling a petri disn only the ____________ of the organism should be written on the ________ of the plate, close to its perphery to prevent itsobscuring observation response.
add info should be written on cover
Definition
name

bottom
Term
to avoid killing the cells and splatering the culture, cool the inaculating wire by ____________________________________ cover prior to obtaining inoculum, or touch the edge of the medium plate
Definition
tapping the inner surface of the culture tube or petri dish
Term
if an ______ culture is used , only tough a single area of growth w/ the inucolating wire to obtain the inoculum
Definition
agar
Term
never ___________ the loope or needle over the entire surface, and take care not to dig in the solid medium
Definition
drag
Term
if a broth medium is used first tap the bottom of the tube against the palm your hand to suspend
Definition
the microorganism
Term
do not tap culture vigourously as this may cause spills or excessive foaming of the culture which may
Definition
denature the proteins in the medium
Term
when using a pipette, use a _____- to obtain and deliver the material to be inoculated
Definition
mechanical pipetting device
Term
in incubation optimum growth occurs @ ______ over a period of 18 to 24 hrs
Definition
37c
Term
petri dishes must always be incubated in an ____________ to prevent water condensation from dropping onto the surface of the culture medium, producing confluent microbial growth
Definition
inverted position (top down)
Term
a culture containing a single unadulterated species of cells id called a
Definition
pure culture
Term
for survival, most microbes ,must use soluable low molecular weight substances that are frequently derived from enzymatic degradation of complex nutrients. a solution containing these nutrients is a
Definition
culture medium
Term
a liquid nedium lacks a solidifying agent and is called a
Definition
broth medium
Term
a broth medium supplemented w/ a solidifying agent called _____________ results in a solid or semi-solid medium
Definition
agar
Term
an extract of seeweed is a complex carbohydrate compossed mostly of galactose, no nutitional value.\excellent solidifying agent cause liquifies @ 100 c and solidifies @ 40c
Definition
agar
Term
a completely solid medium requires an agar concentration of
Definition
1.5 to 1.8
Term
a solution of less than 1% agar results in a
Definition
semisolid solution
Term
while in a liquified state, solid media can be placed in test tubes, which are allowed to cool or harden in a slanted position producing
Definition

agar slants

 

 

Term
agar slants are useful for mainting pure cultures. similar tubes that, following preparation, are allowed to harden in an upright position
Definition
agar deep tubes
Term
used gaseous requirements for microorganisms
Definition
agar deep tubes
Term
agar deep tubes may be liquified in a boiling water bath and poured into petri dishes
provide surface area for the study od microorganisms
Definition
agar plates
Term
the process of a rendering a medium or material free of all forms of life
Definition
sterilazation
Term
glass ______- and glass and or plastic __________ are used to cultivate microoraganisms
Definition
test tubes
petri dished
Term
a suitable medium in the form of broth may be used in the test tube but only a ___________ is used in petri dishes
Definition
solid medium
Term
provide a larger surface area for growth and cultivation. bottom portion contining the medium
top serves as a loose cover
Definition
petri dish
Term
must be carried under aseptic conditions
microorganisms must be transferred from one vessel to another or from stock cultures to various media for maintenence and study
Definition
subculturing
Term
used for aseptic techniques
similar in function to straws (draw up liquid)
the are calibrated to deliever diff volumes depending on requirements.
may be sterilized inside bulk canisters
or wrapped individually in brown paper bag and sterilized in autoclave or dry heat oven
Definition
pipette
Term
used to cultivate microorganisms
provides a rapid uniform transfer of heat to culture vessel, and its agitation provides increased aeration, resulting in acceleration of growth
instrument can only be used in broth medium
Definition
shaking waterbath
Term
how should microscopes be handle
Definition
hold suppot base by one hand and grip the neck w/ the other
Term
when one lens is in focus the other lens will also have the same focal length and can be rotated into position w/out major adjustment
Definition
parafocal
Term
what is the scanning lens magnification
Definition
4x
Term
 can be used to describe any bacterium that has a spherical shape
Definition

coccus

 

[image]

Term
rod-shaped bacteria and a member of the division Firmicutes
Definition

bacillus

 

[image]

Term
is a genus of Gram-negative bacteria possessing a curved rod shape,several species of which can cause foodborne infection, usually associated with eating undercooked seafood
Definition

vibrio

 

[image]

 

 

Term
belong to a phylum of distinctive Gram-negative bacteria, which have long, helically coiled (spiral-shaped) cells
Definition

spirillum/spirochetes

 

[image]

Term
is a genus of Gram-positive rod-shaped bacteria and a member of the division Firmicutes
Definition

[image]

 

individual bacillus

 

 

Term
diplobacil´li a short, rod-shaped organism occurring in pairs. ...
Definition

diplobacillus

 

[image]

Term
 is a genus of aerobic, gram-negative facultative anaerobe bacteria, which grow in culture as rods in chains
Definition

stretobacillus

 

[image]

Term
is a round bacterium (a coccus) that typically occurs in pairs of two joined cells
Definition

diplococcus

 

[image]

Term
is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes[2] and the lactic acid bacteria group. Cellular division occurs along a single axis in these bacteria, and thus they grow in chains or pairs
Definition

streptococcus

 

[image]

Term
is a genus of Gram-positive bacteria. Under the microscope they appear round (cocci), and form in grape-like clusters.[1]
Definition

staphylococcus

 

[image]

Term

4

 

 

[image]

Definition

tetrads

 

[image]

Term
  1. cocci shaped bacteria that fail to dissociate during replication such that they form a cube of eight bacteria.
Definition

sarcinae

 

[image]

Supporting users have an ad free experience!