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increase in number of cells |
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In microbiology, growth is defined as an |
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Bacteria divide by a process known as |
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When a bacterial cell grows to twice their size and form a partition, it is known as |
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The partition from bacterial growth divides the cell into |
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daughter cells relation to parent cells |
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the time it takes for one bacterial cell to divide into two daughter cells |
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environmental conditions and type of bacteria |
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generation time is controlled by |
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clostridium parfringens division time |
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growing bacterial population doubles at a regular interval when under favorable conditions, this is called |
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typical bacterial growth curve |
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a fresh medium is inoculated with a given number of cells and the population growth is monitored over a period of time, plotting the data yields |
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phase of high metabolic activity immediately after inoculation when cell grows in size but NOT population |
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cell starts to divide rapidly; generation time equation can be used |
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limited by food and other nutrients and by space and toxins; byproducts accumulate; growth plateaus |
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cell population decreases |
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chemically-defined (synthetic) medium |
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exact composition is known; uses pure biochemicals; minimum nutritional requirement of bacteria known |
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complex (undefined) medium |
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composition not known; uses complex material of biological origin (beef extract, milk, yeast extract); cultivates unknown bacteria |
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allows growth of one type of bacteria and suppresses others - ex. EMB - allows growth of gram negative bacteria |
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differentiate between different species of bacteria - ex. blood agar differentiates between E. coli and staph epidermis |
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favor the growth of certain bacteria over others - ex. selenite f increases salmonella and decreases E. coli |
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microbial growth can be controlled by heat and UV light |
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microbial growth control by antiseptic, disinfectants, and sanitizers |
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most common and easily available methods for controlling bacterial growth |
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moist heat causes destruction of microorganisms by denaturation of these |
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most common method of moist sterilization |
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samples are placed in a ______________ during autoclaving |
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heated, closed chamber that steam forces air out of the vents or exhausts |
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incineration and flaming are examples of these sterilization methods |
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developed by Louis Pasteur - uses heat to reduce the number of bacteria and all pathogenic bacteria are killed |
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time and temperature for pasteurization |
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121 deg C after pressure of 15psi for 30 minutes |
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time and temperature for autoclave |
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can sterilize surfaces and transparent objects but damaging to plastics |
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antimicrobial agents applied to living tissues/skin |
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antiseptics that destroy bacteria |
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antiseptics that inhibit bacterial growth |
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bacteria that grows in 100 deg C and found in hot springs and deep sea hydrothermal vents |
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bacteria that grows between 45 and 100 deg C and found in hot springs as well as decaying plant matter like peat bogs and compost |
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bacteria that grows in moderate temperatures of -20 and 45 deg C and found widespread in nature |
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bacteria that grows in -10 deg C or lower and found in under sea ice in polar regions |
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bacteria that grows in pH between 5.5 and 8 |
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bacteria that grows in pH<5 |
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bacteria that grows in pH>8 |
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bacteria that requires NaCl for growth |
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bacteria that requires 1-6% salt |
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bacteria that requires 6-15% salt |
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bacteria that requires 15-30% NaCl for growth and found among archaea |
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bacteria that grow at moderate NaCl concentrations but best in the absence of NaCl |
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organisms able to live in environments high in sugar |
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organisms that live in dry environments |
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cannot grow without an abundant supply of oxygen |
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bacteria that require a minimum level of oxygen for growth (1-10%) and well below the 21% found in the atmosphere |
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cannot survive in presence of oxygen |
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organisms that thrive in the presence of oxygen but also grow in its absence |
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indifferent to the presence of oxygen; usually have fermentative metabolism but not harmed by the presence of oxygen |
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destroy all microorganisms including endospores |
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chemicals that kill microorganisms but not necessarily endospores and used on surfaces |
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reduces microbial numbers but do to sterilize |
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