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Micro exam 1
bacteria, viruses, protozoan, fungi, yeasts
78
Microbiology
Undergraduate 2
12/18/2013

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Term
Types of microbes we will study in this course are...
Definition
Viruses, bacteria, algae, protozoa and fungi
Term
Anton Van Leeuwanhoek developed a type of...
Definition
Simple microscope
Term
Louis Pasteur developed the method of sanitizing liquids known as...
Definition
Pasteurization
Term
What did both Louis Pasteur and Robert Koch do?
Definition
Contributed to germ theory of disease
Term
What is the germ theory of disease?
Definition
A particular species of pathogen causes a disease adn must be present in order to cause that disease
Term
Tuberculosis is...
Definition
A re-emerging disease because it is often drug-resistant
Term
Cell theory states that...
Definition
Cells can only come from cells, that is, a cell divides into two cells but can't appear out of nothing
Term
Do prokaryotic cells have a nucleus?
Definition
NO
Term
Are bacteria and archaea prokaryotes?
Definition
Yes
Term
Are prions and viruses prokaryotes?
Definition
Yes
Term
Do eukaryotic cells always have a cell wall?
Definition
No
Term
Is it true that autotrophs require organic molecules for energy and that they depend on heterotrophs to provide these molecules?
Definition
NO
Term
Cells with a nucleus and internal compartments are...
Definition
Eukaryotic
Term
SOME bacterial species can survive long periods in heat, dryness, or lack of nutrients, how do they do this?
Definition
They produce endospores, which germinate when conditions improve
Term
Why do we classify organisms?
Definition
If we group by characteristics then we know something about every organism in that group. Helps us learn faster.
Term
Which of the following cell structures do prokaryotic cells have but eukaryotic do not?
Definition
Nucleoid
Term
A small circular piece of DNA that contains several genes useful to a bacterium is called
Definition
Plasmid
Term
Example of a simple microscope
Definition
Magnifying glass
Term
The distance between two objects at which they appear as one object is
Definition
Resolving power
Term
Which of the following statements about media is true?
Definition
All of the above
Term
A pure culture is...
Definition
Can be tube with one species.
Can be plate with one species.
Can be an individual colony
Is important for research
Term
How can you get a pure culture from a tube with 3 different organisms?
Definition
Streak plate using 3 streak method to get isolated colonies. THen inoculate a new plate with each colony
Term
Which objective lens is the best for observing bacteria?
Definition
100X with oil
Term
The generation time of a bacterial species is...
Definition
Time is takes for a cell to double in size AND divide
Term
What is the energy currency?
Definition
ATP
Term
Series of reactions that converts glucose into pyruvate is...
Definition
Glycolysis
Term
In aerobic respiration, the terminal electron acceptor is oxygen, while in anaerobic respiration teh terminal electron acceptor is something other than oxygen
Definition
true
Term
What is all reactions in the cell?
Definition
metabolism
Term
Is anabolism the reaction in the cell that use energy to MAKE bonds?
Definition
YES
Term
Why does a cell use an electron transport chain?
Definition
To generate a proton gradient to make ATP
Term
What do the extra folds of membrane help nitrifying bacteria do?
Definition
Increase the surface area of membrane, which is where teh nitrifying reactions take place
Term
Organism that prefer extremely high temps
Definition
Hyperthermophiels
Term
Organism that can tolerate high salt...
Definition
Haloduric organisms
Term
Organism that prefer low temps
Definition
psychrophiles
Term
Organisms that prefer acidic conditions
Definition
acidophiles
Term
Organisms that need higher concentrations of CO2
Definition
Capnophiles
Term
When bacterial chromosome is copied by DNA polymerase
Definition
replication
Term
Which of the following statements about DNA are true?
Definition
All of the above
Term
Experiment by Avery, Macleod and McCarty, where heat-killed S strain bacteria were sepearated into componenets showed
Definition
R strain acquired something from dead S strain that allowed them to be virulent
Term
Gener that is always turned on (always making protein product) is...
Definition
Constitutve gene
Term
Conjugation
Definition
One way flow of DNA
Term
How can bacteria develop resistance to a particular antimicrobial agent
Definition
Chromosomal mutation or acquisition of new gene
Term
Process by which some bacteria can take up DNA from environment
Definition
Transformation
Term
Results in some phage with DNA from the bacterial chromosome INSTEAD OF viral genes
Definition
Generalized Transduction
Term
Results in some phage with DNA from the bacterial chromosome IN ADDITION to viral genes
Definition
Specialized Transduction
Term
Bacterial Sex
Definition
Conjugation
Term
Which of the following processes do NOT include viruses
Definition
Transformation
Term
A mutation taht results in a stop codon
Definition
Nonsense mutation
Term
Mutation that results in a different amino acid
Definition
Missence mutation
Term
Mutation that results in no change to the protein
Definition
Silent mutation
Term
Insertion or deletion of 1 or 2 nucleotides
Definition
frameshift mutatino
Term
Microbe benefits
Definition
Produce food
Degrade wastes
Biogeochemical cycles
Natural microflora
Term
Legumes
Definition
Harbor and feed nitrogen fixing organisms in nodules in their roots
Term
Bubbles in bread and gas released from fermenting wine is
Definition
Carbon dioxide
Term
Live microorganisms which when administered in sufficient quantities may improve health are
Definition
Probiotics
Term
Associated with mad cow in humans
Definition
Variant Creutzfeld-Jacob disease
Term
Prions cause disease by
Definition
Destroying nerve tissues by causing protein aggregates
Term
Which of the following is not one of the three virus coat shapes
Definition
Bacillus
Term
What are the ways we classify viruses
Definition
nucleic acid, viral coat, organism it infects, tissue it infects
Term
Virus or nucleic acid enters cell
Definition
Penetration
Term
Nucleic acid is copied, and viral components are made
Definition
Replication
Term
Virus attaches to cell by binding ot specific receptors
Definition
Adsorption
Term
Viruses escape from cell
Definition
Release
Term
Complete viruses are built
Definition
Assembly
Term
Prion disease aka
Definition
Transmissible spongiform encephalopathy
Term
Viruses have DNA or RNA as their genetic material but NOT both
Definition
true
Term
All viruses have an envelope
Definition
false
Term
Viruses have to attach or adsorb to a cell before they can infect
Definition
true
Term
Phage therapy is a treatment in which we use a virus specific to bacteria to control bacterial infections
Definition
true
Term
A temperate or lysogenic phage
Definition
Can either replicate and kill cell or integrate into genome and wait.
Term
Gram positive characteristics
Definition
Thick cell wall
Stains purple
No outer membrane
Term
Gram negative characteristics
Definition
Thin cell wall
Stains pink
Outer membrane
Term
Difference between simple and differential staine
Definition
Simple shows shape
Differential shows different groups?
Term
Cell morphology means
Definition
Shape
Term
Motility medium tests organisms ability to
Definition
swim away from inoculation point
Term
DOC media is
Definition
selective and differential
Term
Describe tube to plate transfer
Definition
3 streak method
Term
What two groups does acid fast stain show us how
Definition
Acid fast are red
Non acid fast are blue
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