Term
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Definition
1. influenza- spanish flu 1918 - crossed the country in 3 days, over 100 million deaths
2. Four corners disease- Western state - native americans come down with pulmonary disease - hantavirus and sin nombre
3. Norovirus - from cruise ships - vomiting and diarrhea; morbidity |
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Term
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Definition
- related to four corners disease
- found in rodents
- sin nombre is the strain that jumped the species barrier
- increased rate of mortality |
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Term
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Definition
1. host range
2. size
3. virion type
4. viral symmetry
5. nature/expression of the genome
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Term
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Definition
- what types of organisms are effected
- bacteriophage - virus that infects bacteria only
- eukaryotic cells like protozoa, plants, fungi and algae are effected
- viruses that effect animals may or may not effect us |
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Term
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Definition
- electron or light microscopy
- very small (molecular size) to large (barely visible under light microscopy)
- poxvirus and mimivirus are the 2 largest viruses
- mimivirus is a potention evolutionary bridge
- hemoglobin is one of the smallest
- how large virus is reflect how much genetic information they have; more info, less dependent |
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Term
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Definition
- nucleic acid/viral core
- protein capsid
- naked virus: nucleocapsid, less susceptible
- enveloped virus: ether sensitive, envelope around protein coat, no effective without enveloped (cannot infect or replicate)
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Term
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Definition
- AKA spikes
- on the envelope
- not all viruses have them
- important to coronavirus (SARS)
- crown-like appearence
- imporant for attachment |
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Term
Viral structural organization |
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Definition
- nucleic acid core
- proten capsid
- capsid is made up of capsomers - discreet, small, protein subunits
- capsomers are made up of protomers
- virion is the entire infected particle made up of all the parts listed |
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Term
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Definition
- TMV- tobacco mosaic virus
- helical symmetry: capsomers attached to core and compressed in a circle because of envelope
- helical structure is also filamentous |
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Term
Neuraminidase & hemagglutin |
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Definition
Ne or N / He or H
- orthomyxoviridae
- change on an annual basis
- why we are immunized every year- different strains
- ex) h1n1, h1n2, h3n1 etc.
- cause of antigenic drift |
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Term
Antigenic shift vs. anigenic drift |
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Definition
shift: major changes that can occur in the antigens of a virus -- epidemics
drift: slight changes that occur in the antigens of a virus; specific antibodies made in response to the antigen before the change occurred are only partially protective -- exterior of virus shifts away |
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Term
Icosahedral vs. complex/binal symmetry |
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Definition
- i: cubic, regular, polyhedral, isometric
--> 12 vertices/points, 20 faces, 30 edges
--> pentons at points, hexons at edges
--> capsomers not attached to core, core is free
--> more volume/surface area
--> no filaments
- complex/binal: reminiscent of bacterial cells with nucleoid ex) poxvirus |
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Term
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Definition
- RNA vs. DNA
- double vs. single stranded
- circular vs. filamentous
- segemented
- both can never make up core; one or the other
- monopartit vs. multipartite |
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Term
Dependent/polymerase combinations |
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Definition
- DNA dependent/RNA polymerase: produces RNA from DNA
- RNA dependent/DNA polymerase: produces DNA from RNA
--> AKA retrovirus
--> reverse transcriptase
--> oncogenic- capable of causing cancer |
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Term
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Definition
RNA- single stranded
- RNA +: mRNA transcription, translated by eukaryotic cell
- RNA-: needs to be manipulated/altered so a + sense can be produced from it; replication intermediate |
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Term
How do we prevent drug resistance? (7) |
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Definition
1. educate the public- viral vs. bacteria, costs
2. antibiotic sensitivity testing- avoid encouraging resistance
3. use narrow spectrum compounds- avoid super infection
4. use combinations (synergy)
5. rigidly contain resistant organism (isolation/quarantine)
6. take all required medication on schedule - on time to maintain constant levels, full days supply
7. restrict use of therapeutically valuable antimicrobics for nonmedical purposes- ex) cattle - leads to food poisoning |
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Term
Mechanism of bacterial resistance to antibiotics (7) |
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Definition
1. enzymatic cleavage
2. chemical modifations- inactivation
3. reduced uptake- due to mutation
4. active efflux of antibiotic from cell- pumped out as same rate of entry
5. eliminate/reduce binding to target
6. metabolic bypass of inhibited reactions(salvage pathway)- bypass, shunt, work around
7. overproduction of target- ratio of enzyme/metabolite to antimicrobial (sulfa/PABA) |
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Term
Chemical modification / inactivation (3 ways) |
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Definition
1. acetylation
2. phosphorylated
3. adenylated |
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Term
Development of antibiotics resistance (3) |
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Definition
1. spontaneous mutation- vertical gene transfer
2. gene transfer- horizontal gene transfer; includes conjugation, transformation, transduction
3. plasmid promiscuity- transfer is extensive; r-plasmids |
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Term
Viral replication based on genome |
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Definition
6 types:
1.+/- DNA
2. - DNA
3-5 RNA
6. reverse transcriptase |
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Term
Replication intermediates |
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Definition
dsDNA
ssDNA
dsRNA
ssRNA
* intermediate needed to get to + mRNA
* important in synthesis/replication |
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Term
Viral replication steps (6) |
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Definition
1. Adsorption/attachment
2. Penetration
3. Uncoating- liberation from capsid, protease/protein splitter
4. Synthesis/replication
5. Assembly/maturation
6. Release
* time varies; rhino and adenovirus can take as little as 24 hours, others take years |
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Term
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Definition
- naked virus: receptors on capsid
- enveloped virus: receoptrs on envelope
- adsorption can be increased by density or amount of viruses |
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Term
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Definition
- Neurotropic: rabies- effects neurons, localized in NS
- Pneumotropic: influenza- effects respiratory epithelium
- Viscerotropic: rotavirus, noravirus, poliovirus- effects intestinal epithelium
- Immunotropic: HIV- t-helper cells (on CD4 cells) become infected and dysfunctional
- Cytomegalovirus: effects many systems- epilthelium, monocytes, lymphocytes
- EB: epstein barr- mononucleosis (mono) |
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Term
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Definition
- depends on kind of virus and if it is enveloped
- can identify viruses by probes due to proteins
- penetration and uncoating can occur simultaneously |
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Term
Penetration of naked virus vs. enveloped virus |
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Definition
- naked: not just endocytosis; capid is unstable
- enveloped: endocytosis and fusogenic proteins on plasma membrane
--> endocytosis forms double membrane |
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Term
DNA vs. RNA synthesis/replication |
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Definition
RNA- in cytoplasm
DNA- in nucleus
* exceptions: varcinia (cowpox), variella (smallpox) |
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Term
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Definition
+ssDNA, AKA +D1
-ssDNA, AKA -D1
* +/- ssRNA forms dsDNA intermediate to make mRNA with help of reverse transcriptase from +ssRNA
* dsDNA can jump in whenever it wants; bad results
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Term
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Definition
+ssRNA- AKA +R1
--> can be used directly to form +mRNA at right polarity and sense
--> template to form -ssRNA
--> +/- ssRNA combination makes a lot of +mRNA
-ssRNA- AKA -R1
--> needs RNA dependent RNA polymerase
--> contributes to translation
--> second intermediate may be needed to reach genome |
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Term
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Definition
1. early transcription
2. early mRNA
3. early protein
* switches to late once viral cores have been synthesized (genome copies)
* compromises host cell as it is making virus instead of maintaining the cell (morphological changes) |
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Term
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Definition
- cytopathic effects
- viral induced damage to infected cell that alters microscopic appearence
- occurs during synthetic phase |
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Term
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Definition
1. cell rounding- lose membrane
2. cell lysis
3. nuclear pyknosis- nucleus becomes coarse and clumps together; intense stain
4. Nuclear/cytoplasmic inclusion bodies
5. Multinucleated giant cell formation |
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Term
Multinucleated giant cell formation |
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Definition
- AKA: syncytial formation
- nuclei cluster in middle or periphery
- negri bodies- neuronal tissue characteristic of rabies
- basophilic bodies- characterization of hepatitis (liver tissue) |
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Term
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Definition
- varies with different viruses
- core encased in capsid material
- can be self assembling (encapsidation)
- location varies as well; some will assemble where they undergo synthesis
ex) Dna=nucleus, Rna=cytoplasm |
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Term
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Definition
- AKA: productive or lytic
- virus remains localized and disappears when disease ends
- activitity shutdown; cells killed (rupture)
- release of virus is dependent on cell dying
- virus is released after cell lyses |
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Term
Multiplicity of infection |
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Definition
MOI
- number of virus particles that are released
- range for 10 to thousands |
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Term
Budding in viral infections |
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Definition
- enveloped viruses
- cell membrane is modified by removing proteins and adding virus matrix proteins (exit proteins)
- phospholipid bilayer is left in tact
- virus matrix proteins fuse to cell membrane and undergo endocytosis |
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Term
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Definition
- neuraminidase cleaves sialic acid on the membrane/envelope in order to release virus
- neuraminidase inhibitors limit influenze infection |
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Term
Persistent viral infection |
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Definition
- virus that is released by budding usually survives
- viral infection does not always result in death, just alteration
- establishes infection and remains for a long time period
- no symptoms
- may or may not cause disease; person can be a potential source of infection |
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Term
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Definition
- oncogenic- capable of causing cancer
- damage to cells can lead to tumor cell formation
- virus integrates into host cell chromosome
- can jump in anywhere: regulatory protein, plasmid or chromosome |
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Term
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Definition
- integrate into host of chromosome but no abnormal changes of host cell (provirus)
- can cause infection by plasmid replication
- symptomless period followed by reactivation |
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Term
Reactivation of viral disease (4) |
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Definition
1. latent virus
2. Herpes simplex virus (HSV-1, HSV-2)- cold sore, fever blister, lesion that is reactivated due to stress, additional disease, or depressed immune system
3. VZ virus- varicella zoster - chicken pox is reactivated as shingles (herpes virus, not poxvirus)
4. EB- ebstein barr- mononucleosis and burkitt's lymphoma (hepes virus) |
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Term
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Definition
- role of viruses and cancers:
1. bittner mammary tumor virus
2. gross murine leukemia virus
3. raus sarcoma virus |
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Term
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Definition
- role of viruses and cancers:
1. t-cell leukemia caused by human t-cell leukemia virus type 1 (RNA)
2. Burkitts lymphoma caused by epstein barr (DNA)
3. Nasopharyngeal carcinoma caused by epstein barr (DNA)
4. Hepatocellular carcinoma caused by hepatitis B (DNA)
5. skin and cervical cancer caused by papilloma (DNA) |
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Term
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Definition
- need living systems since viruses are obligate intracellular parasites
1. laboratory animals
2. embryonated eggs
3. cell, tissue and organ cultures- Robert Enders |
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Term
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Definition
- membrane- herpes, pox, raus sarcoma
- amniotic- influenza, mumps
- yolk sac- herpes
- allantoic- influenza, mumps, newcastle, adenovirus |
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Term
Colony forming units calculation |
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Definition
# of colonies / 1
x 1 / aliquout
x 1 / microliter amt
ex) 74/1 x 1/10^-6 x 1/100 microliters
7.4x10^1 x 10^6 x 10^1 = 7.4x10^8 CFU/mL |
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Term
Axenic, pure, mixed cultures |
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Definition
a: culture that contains a signle known genus and species of bacteria
p: signle genus and species of bacteria but identity of the organism may not be known
m: two different types of bacteria |
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Term
E. coli, Serratia marcesens, Micrococcus luteus colors |
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Definition
e: cream, white colonies
s: red colonies (from prodigosin)
m: yellow colonies
* use tstreak technique to streak for isolation from mixture |
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