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Micro CH 9
Taxonomy
42
Biology
Undergraduate 2
03/23/2011

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Term
taxonomy
Definition
science of classification
Term
Carolus Linnaeus
Definition
Swedish botanist credited w/founding science of taxonomy
originated binomial nomenclature
Term
binomial nomenclature
Definition
system of taxonomy developed by Linnaeus in which each organism is assigned a genus and specific epithet
Term
A name of a bacterium often reveals what?
Definition
its shape, where it is found, what nutrients it needs, who discovered it, what disease it causes
Term
strain
Definition
supgroup of a species with one or more characteristics that distinguish it
Term
How is a specific strain identified?
Definition
by name, number, or letter following specific epithet
Ex. E. coli O157:H7
Term
3 reasons why taxonomy is important
Definition
1) identification of organisms
2) classification
3) gives info for evolutionary change
Term
dichotomous key
Definition
tool of taxonomy that presents an "either-or" choice system to classify organisms
Term
problems with taxonomy
Definition
1) goal is to classify by phylogenetic relationships but this is difficult b/c evolution is continuous and rapidly occurring, and knowledge of evolutionary history of bacteria is incomplete
Term
5 kingdoms of organisms
Definition
1. Plantae
2. Fungi
3. Animalia
4. Protista
5. Monera
Term
4 characteristics of life
Definition
1. all organisms composed of cells
2. all carry out certain functions (metabolism)
3. cell is basic unit of life
4. all cells bound by cell membrane, have DNA, ribosomes, lipids, and carbs
Term
Kingdom Monera
Definition
unicellular
prokaryotes
lack true nuclei
lack membrane bound organelles
DNA has little/no protein
reproduce by binary fission
3 types: Eubacteria, Cyanobacteria, Archaeobacteria
Term
Kingdom Protista
Definition
diverse group (algae, protozoa, euglena)
unicellular
eukaryotes
true nucleus
membrane bound organelles
some cause disease
do not develop from embryo like plants/animals or spores like fungi
Term
Give examples of Protists that cause disease.
Definition
Plasmodium-->malaria
Amoeba-->dysentary
Term
Kingdom Fungi
Definition
unicellular & multicellular
eukaryotes
form spores not seeds
some cause disease
Term
Give examples of Fungi that cause disease
Definition
Candida albicans-->yeast infection
Aspergillus niger-->black mold
Cryptococcus neoformans
Term
Kingdom Plantae
Definition
multicellular
eukaryotes
photosynthesize
have chlorophyll
some produce antimicrobial substances
some susceptible to fungal, viral, bacterial infections
Term
Kingdom Animalia
Definition
mostly macroorganisms, but still studied b/c can be carries of microorganisms
all develop from zygotes
multicellular
eukaryotes
some parasites
Term
Give examples of Animalia that are parasites.
Definition
helminth worms (tapeworms, flukes, roundworms)
arthropods (ticks, mites, lice, fleas)
Term
3 domains of organisms
Definition
Bacteria, Archaea, Eukarya
Term
stromatolites
Definition
fossilized photosynthetic prokaryotes that appear as masses of cells or microbial mats
studying these shows that 3 separate domains arose 3 billion years ago
Term
tree versus shrub view of evolution
Definition
proposed by W. Ford Doolittle
said that evolution had many roots rather than 1 ancestral line and branches crisscross/merge, which occurred through lateral gene transfer
Term
viruses
Definition
acellular infectious agents smaller than cells
have nucleic acid (DNA or RNA)
nonliving
cause disease not placed in a kingdom
Term
What types of characteristics are viruses classified by?
Definition
shape, type of n.a, presence of envelope, enzymes, and tail structure
Term
Why is virology important?
Definition
1) techniques are derived from micro techniques
2) viruses are a concern to health scientist b/c of disease
Term
Why is the classification based on morphology not helpful for prokaryotes?
Definition
prokaryotes have few structural characteristics and they are rapidly changing
Term
numerical taxonomy
Definition
comparison of organisms based on quantitative assessment of large number of characteristics
Term
taxonomic methods for bacteria
Definition
numerical taxonomy
genetic homology
ribosomal gene sequencing
immunology
phage typing
Term
What are some methods of studying genetic homology of bacteria for taxonomic purposes?
Definition
direct methods: base composition comparison, DNA/RNA sequencing, DNA hybridization
indirect methods: protein profiling
Term
base composition comparison
Definition
a direct method of studying genetic homology for taxonomic purposes
comparing percentages of bases present in DNA
Term
DNA/RNA sequencing
Definition
a direct method of studying genetic homology for taxonomic purposes
probes are used to identify specific DNA sequences, to which they attach and help in identification of specimen
Term
probes
Definition
SS DNA fragments that have sequences complementary to those being sought
Term
DNA hybridization
Definition
a direct method of studying genetic homology for taxonomic purposes
process in which DS of DNA of each of 2 organisms are split apart and allowed to anneal
degree of annealing is directly proportional to quantity of identical DNA
Term
protein profiling
Definition
an indirect method of studying genetic homology for taxonomic purposes
lab-prepared pattern of proteins found in a cell, which are unique to each organism, is prepared by electrophoresis
bands at same location indicate same protein is present in 2 cells
Term
ribosomal gene sequencing
Definition
taxonomic method in which degree of similarity in 16S rRNA sequences indicates evolutionary relatedness
Term
immunology as a taxonomic method
Definition
monoclonal antibodies are studied, which bind to specific protein on cell surface
if they bind to more than 1 organism, organisms have protein in common
Term
phage typing as a taxonomic method
Definition
use of bacteriophages, which cause holes in bacterial lawn, allowing identification of a strain
strains lysed by same phages are closely related
Term
divergent evolution
Definition
process in which descendants of a common ancestor species undergo sufficient change to be identified as separate species
Term
species
Definition
collection of strains that share many common features and differ significantly from other strains
Term
type strain
Definition
original reference strain of a bacterial species, descendants of a single isolation in pure culture
namebearer of species, preserved in culture collections
Term
Bergey's manual
Definition
internationally recognized reference for bacterial taxonomy
uses dichotomous key and physical characteristics
does not present accurate picture of evolutionary relationships, but instead practical groups of bacteria for easy identification
Term
problems with bacterial taxonomy
Definition
too little is known about evolutionary relationships
some groups contain unusual organisms
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