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Micro Ch 7 Test questions
Microbial growth possible test questions
55
Microbiology
Undergraduate 2
06/28/2010

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Term
after 7 days the dry weight of fungi on 'apples' is 5mg, you conclude...
Definition
the compound is fungistatic
Term
decimal reduction time is the
Definition
time in minutes that 90% of pupulationat a given temp is gone
Term
sterilization is
Definition
removal of all microorganisms
Term
difference between a disinfectant and an antiseptic
Definition
disinfectans are used on envir suf and antiseptics are used on living tissue
Term
what treatment achieves sterilization
Definition
autoclaving
Term
name 3 antiseptics... 1 not
Definition
iodine, bisphenol, alcohol are-soap is not (all are used on skin)
Term
why is soap not ann antiseptic
Definition
because soap does not kill or inactivate microbe
Term
what kills cells by damaging dna?
Definition
uv
Term
what condition would interfere with the effectiveness of an antiseptic or disinfectant
Definition
presence of biofilms
Term
what is typically destroyed by antimicrobial agents (3 target agents)
Definition
plasma membranes, proteins, nucleic acids
Term
what are these not? lyophilization, deep-freezing filtration
Definition
bacterialcidal
Term
what substance is effective against bacterial endospores
Definition
chlorine bleach
Term
what is th most effect conc of ethyl slcolhol?
Definition
75%
Term
what organism is the most difficult to kill
Definition
prions
Term
is triclosan an oxidizing agt?
Definition
No Oozone, hydr pero, chlorine)
Term
what is the most useful disinfectant for med instruments
Definition
glutaraldehyde..
Term
Iodine is considered a ?
Definition
antiseptic (betadine)
Term
which organisms is generally easy to kill
Definition
lipophilic viruses
Term
if the disinfectants are nontoxic and designed for the same purpose what is the most effect?
Definition
z-1:1,000.. the disinfectant with the greast use-dilution value
Term
disk-diffusion test 'which gives the best dilution...
Definition
not enough info
Term
why does dairy creamer etc not have to be refridg?
Definition
IT IS TREATED WITH.....UHT treatment, ultra high temp
Term
antiseptic is used to remove microbes from
Definition
the skin before an injection
Term
what agent challenges sterilization
Definition
prions
Term
what is the term for inhibiting growth
Definition
bacteriostatic
Term
what is not a major target for antimicrobials
Definition
flagella
Term
what treatment is most effective against the contaminated counter
Definition
heat, 52C
Term
what are char of autoclave
Definition
use of moist heat, high pressures, high temp, ability to sterilize solu w/endospores
Term
pasteurization was first used by pasteur to control spoilage of
Definition
wine
Term
what i sappropriat for a medium for growing fasidious bacteria?
Definition
autoclave the medium pror to adding growth factors, cool, aseptically add the growth factors
Term
what is least likely to NOT be damaged by gamma radiation
Definition
prions
Term
name food preservation methods
Definition
deep freezing, osmotic pressure, commercial canning, dessication
Term
what is the easiest to kill with microbial agents?
Definition
e.coli
Term
what radiation is least effective in killing microbes
Definition
microwave
Term
DRT for a part bact spe at 60C is 30 mins, how long would it take at this temp to ___ bacterial pop
Definition
90 minutes
Term
alcohol is most effective at what %
Definition
70%
Term
examples of microbial control with heavy metal... (4)
Definition
algae control in pools with copper sulfate, burn treat w/silver sulf, mouthwash w/zinc chloride, antiseptic sol containg mercurochrome (NOT benzol peroxide)
Term
a compound found in antimicrobial soaps that targets g-pos bact..
Definition
triclosan
Term
list possibilities of additives in snack food
Definition
(xanthan is not) pot sorbate, sodium benz, calc prop, sorbic acid
Term
Most viruses are highly resistant to disinfectant and antiseptics, t or f
Definition
false
Term
presence of organic matter such as blood or saliva may impair the action of antimicrobial chem t or f
Definition
t
Term
all bacteria die at once when they are exposed to heat or chem treat t or f
Definition
f
Term
times reeq for sterilization in an autoclave are shorter than those req in a dry oven because moist specimens mor effec than does dry heat (t or f)
Definition
t
Term
ethylene oxide gas is used to sterilize medical equip that might be damaged by exp of others
Definition
t
Term
tor f, uv radiation and xray rad are similar in that they damage dna and have a high penetrating power
Definition
f
Term
iodine is one of the least eff antimicrobial chem, effec only agains certain very sensitive t or f
Definition
f
Term
ionizing radiation can be used to sterilize some foods such as spices, meat, and fruits and veget t or f
Definition
t
Term
oxidizing agents limit the growth of
Definition
anaerobic bact
Term
4 types of bacteria may survive pasteurization they are....
Definition
thermoduric, endospore, heat resistant
Term
___ ammonium cmpds are less effective against g-neg bacteria and more efficient with g-pos
Definition
quaternary
Term
what term inhibits growth
Definition
homeostatic
Term
if an agent is fungistatic it is??
Definition
it would slow growth as the treatment
Term
what household substance is effective against endospores
Definition
chlorine bleach
Term
This chemical is not an ooxidizing agent, it inhibits enzyme req for synthesis of lipids
Definition
triclosan
Term
name a couple of oxidizing agents
Definition
chlorine, hydrogen peroxide, ozone
Term
what is the order of death for these bacteria by chemical agents?
g positive and negative, endospores, mycobacteria
Definition
g pos g neg, mycobacteria, endospores
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