· Release of high speed subatomic particles or waves of electromagnetic energy
o Particulate radiation
o Electromagnetic radiation – given off as waves by decomposing atoms and travels at speed of light
· Depends on wavelength, intensity, duration
o Some harmless (visible light)
o Other kills
§ Ionizing
· High energy radiation that creates radicals
o Damage DNA and cell components
· Produced by
o Electromagnetic waves -- Gamma rays and X-rays (less than 1nm wavelengths)
o Particulate radiation -- Electron beams
· Electron beams have high energy and sterilize quickly but penetrate power is low
o Sterilize disposable tissue culture and med supplies but not thick objects
· Gamma rays and X-rays have high energy and penetrate deeply
o Require hours to kill microbes
o Can preserve food but public has been reluctant to embrace
§ Nonionizing
· Longer than 1 nm
· UV Light, visible light, infrared waves, microwaves, radiowaves
· UV only non-ionizing electromagnetic radiation that is microcidal
o Between 40 and 310 nm
o Kills at 200-295nm by altering DNA bases
§ Thymine dimers
o Maximal killing at 260 nm which is light absorption optimum of DNA
o Disinfects things that come in direct contact with it
§ Disinfects air and uncovered nonporous surfaces in operating rooms, food cafeterias
o Low penetrating power
§ Doesn’t penetrate plastics, paper, cellophane, cannot sterilize wrapped or covered materials
o Harmful to humans
§ Sunburns, skin cancer, damage to retinas
§ Other Non-ionizing radiation
· Sunlight has some antimicrobial effect
o Forms singlet oxygen
o Ozone screens out ionizing radiation
· Microwaves
o Don’t directly damage microbes
o Kills vegetative cells by heating water in cytoplasm of cells
Too inefficient and unreliable for sterilization |