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MICR33
Chapter 10
42
Microbiology
Not Applicable
07/17/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Taxonomy
Definition
the science of classifying organisms. provides universal names for organisms and a reference for IDing organisms
Term
Systematics, or Phylogeny
Definition
the study of evolutionary history of organisms

All species of Inventory (2001-2005)- to ID all species on Earth
Term
Placing bacteria: 1735
Definition
kindoms: plantae and animalia
Term
Placing bacteria: 1978
Definition
two types of prokaryotic cells found
Term
3- Domain System
Definition
Bacteria, Archaea, & Eukarya
Term
Characteristics of Archaea
Definition
cell type: prok; cell wall: no peptidoglycan; no antibiotic sensitivity
Term
Characteristics of Bacteria
Definition
Prokaryotics; contains peptidoglycan in cell wall; uses amino acid formylmethionine; sensitive to antibiotics;
Term
Characteristics of Eukarya
Definition
Euk; cell wall contains carbs; not sensitive to antibiotics
Term
stromatolites
Definition
rocklike pillars formed by bacterial communities began growing about 3000 years ago
Term
phylogenetics
Definition
each species retains some characteristics of its ancestor; grouping organisms according to common properties implies that a group of organisms evolved from common ancestor. (anatomy, fossils, rRNA)
Term
scientific nomenclature
Definition
common names vary with languages and geography.
binomial nomenclature= (genus+specific epithet)
- used worldwide
Term
taxonomic hierarchy
Definition
domain>kingdom>phylum>class>order>family>genus>species
Term
classification of prokaryotes: prokaryotic species
Definition
a population of cells with similar characteristics
Term
prokaryotic species: culture
Definition
grown in laboratory media
Term
prokaryotic species: clone
Definition
population of cells derived from a single cell
Term
prokaryotic species: strain
Definition
genetically different cells within a clone
Term
Eukaryotic species
Definition
a group of closely related organisms that breed among themselves
Term
animalia
Definition
multicellular, no cells walls, chemoheterotrophic
Term
plantae
Definition
multicellular, cellulos cell walls, usually photoautotrophic
Term
fungi
Definition
chemoheterotrophic, unicellular or multicellular, cell walls of chitin, develop from spored or hyphal fragments
Term
protista
Definition
a catchall kingdom or euks that do not fit in other kingdoms. grouped into "clades" based on RNA
Term
viral species
Definition
population of viruses with similar characteristics that occupies a particular niche
Term
references: International Journal of Systematic and Evolutionary Microbiology
Definition
Articles with evidence of new species or classification
Term
references: Bergey's Manual of Systematic Bacteriology
Definition
Provides phylogenetic and identification information on bacteria and archaea
Term
references: Approved lists of bacterial names
Definition
lists species of known prokaryotes based on published articles
Term
classification
Definition
placing organisms into groups of related species- lists of characteristics of known organisms
Term
identification
Definition
matching characteristics of an "unknown" organism to lists of known organisms - clinical lab ID
Term
Bergey's manual of determinative bacteriology
Definition
provides ID schemes for IDing bacteria and archaea and provides phylogenetic information on bacteria and archaea
Term
ID methods: morphological characteristics
Definition
useful for IDing eukaryotes, limited for prokaryotes
Term
ID methods: differential staining
Definition
gram staining or acid-fast staining
Term
ID methods: biochemical tests
Definition
determines presence of bacterial enzymes
Term
Enterotube II from Becton Dickinson
Definition
one type of rapid ID method for bacteria
Term
serology
Definition
combine known antiserum plus unknown bacterium. different tests/procedures: slide agglutination test, ELISA, Western blot
Term
ELISA
Definition
enzyme-linked immunosorbent assay: known antibodies, unknown type of bacterium, antibodies linked to enzyme, enzyme substrate
Term
positive direct ELISA
Definition
to detect antigens
Term
positive indirect ELISA
Definition
to detect antibodies
Term
Flow Cytometry
Definition
uses differences in electrical conductivity between species; fluorescence of some species' cells selectively stained with antibody plus fluorescent dye
Term
DNA base composition
Definition
guanine & cytosine moles % (GC)
Term
DNA fingerprinting
Definition
electrophoresis or restriction enzyme digests
Term
DNA chip
Definition
can contain hundreds of thousands of synthetic single-stranded DNA. unknown DNA from sample is separated into single stands, cut, and labeled with fluorescent dye
Term
DNA chip 2
Definition
the unknown DNA is allowed to hybridize with the DNA on chip and bind only to the complementary DNA. DNA will be detected by fluorescence
Term
FISH
Definition
fluorescent in situ hybridization
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