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Definition
enzyme multiplied immunoassay - homogenous. Sample analyte competes with enzyme labeled antigen for binding site on antibody.If there's a lot of analytes, there'll be less labeled binding which means that they'll be free to respond to analytes for spectrophotometer reading. NADH production.
1) substrate (glucose6PO4), coenzyme (NAD+) and antibody.
2) enzyme labeled drugg and buffer |
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Definition
Serum (FPIA/photometric called trinder)
Trinder: react it with Fe3+ to form color complex at 540 nm. |
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Term
| Primary testicular failure |
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Definition
| Increased LH, FSH due to decreased testerone (hypergonadotropic). |
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Definition
| distinguish between cushing's syndrome and disease. Adrenal tumor = no ACTH. Ectopic = lots of ACTH. |
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Term
| Dexamethasone suppression test |
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Definition
| 1 mg dex at night. Collect at 8 am. Normal results is when its low <3. Cushing's is elevated. Cortisol is usually not supressed in non pituitary tumors.It is a basically does a negative feedback on the hypothalamus than cortisol, but with cushing's it doesn't work. |
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Term
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Definition
| plasma cortisol and ACTH levels and stimulation helps diagnose primary adrenal insufficiency. |
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Definition
| with plasma cortisol and ACTH level, helps diagnose if its secondary or tertiary adrenal insufficiency. |
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Term
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Definition
24 hour urine free cortisol - if elevated that means its ( over 120) cushing's syndrome.
Diurenal variation - check plasma at 8 am (high) and 4 pm (low). |
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Term
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Definition
| produced by anterior pituitary in response to low free T4 or T3. A normal TSH rules out thyroid disease. TSH is low in primary hyperthyroidism and high in primary hypothryodism. |
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