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Metabolism and Substrate Use
Metabolism and Substrate USe
102
Physiology
Undergraduate 4
02/13/2009

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Cards

Term
What are the main forms of energy inside the body?
Definition
Heat, Chemical, Mechanical, Light, Sound, Entropy, free energy
Term
What is delta G?
Definition
Measure of innate directionality and theoretical free energy release of a chemical reaction
Term
What direction do all reactions proceed in?
Definition
Proceed in direction of increased entropy and release of free energy
Term
What is entropy?
Definition
A scientific measure of disorder
Term
What sign does free energy carry?
Definition
Negative sign
Term
What is the splitting of ATP called?
Definition
Hydrolysis
Term
What are the products of ATP hydrolysis?
Definition
ADP (adenosine diphosphate) and Pi(inorganic phosphate)
Term
What is the standard free energy of hydrolysis under test tube conditions?
Definition
-7.3 kcal*mol-1
Term
What is the standard energy of hydrolysis within the body?
Definition
-11 kcal*mol-1
Term
What cellular source of immediated energy provides a reserve of phosphate energy to regenerate ATP?
Definition
Creatine Phosphate
Term
What ennzyme catalyzes the reaction between Creatine phosphate and ADP?
Definition
Creatine Kinase
Term
Which enzyme has the ability to generate one ATP and two ADPs?
Definition
Myokinase
Term
What is yielded from a reaction of 2 ADPs and myokinase?
Definition
ATP + AMP
Term
What are the three sources of immediate energy?
Definition
ATP, Creatine Phosphate, and Myokinase(adenylate kinase)
Term
How many ATPs are generated through glycolyisis?
Definition
2
Term
When are nonoxidative energy sources called upon?
Definition
When muscle contraction lasts more than a few seconds
Term
What does glycolysis inevitably result in?
Definition
Lactate
Term
What are potential oxidative energy sources for muscle?
Definition
Sugars, CHO, fats, and some amino acids
Term
How many ATPs are produceds through oxidated metabolism?
Definition
36
Term
What is the only way fat can be catabolized?
Definition
Through oxidative processes
Term
What is the only way amino acids can be catabolized?
Definition
Through oxidative processes
Term
What is transamination?
Definition
Switching nitrogen in amino acids to another compound
Term
Where does nitrogen removal occur?
Definition
The liver
Term
What is a modulator which slows enzymatic function?
Definition
Inhibitors
Term
Is ATP an inhibitor or a stimulator?
Definition
Inhibitor
Term
What does ATP inhibit in resting muscle?
Definition
CHO, Fat, and amino acid breakdown
Term
What is the product of PCr +ADP with use of enzyme Creatine Kinase?
Definition
ATP + Cr
Term
What is the myokinase reaction?
Definition
ADP+ADP->myokinase->ATP + AMP
Term
Where are ATP and PCr stored?
Definition
Within contactile mechanisms
Term
How long are ATP-PCr activities?
Definition
<1-6 seconds
Term
What makes protein difficult to catabolize?
Definition
Nitrogen
Term
Where do oxidative reactions take place?
Definition
Withiun the mitochondria
Term
Where does glycolysis occur?
Definition
Within the cytosol
Term
What is the main enzyme involved in glyconeogenolysis?
Definition
Phosphorylase
Term
What is the main allosteric enzyme regulating glycolysis?
Definition
Phosphofructokinase
Term
What temperature can denature many enzymes?
Definition
>60 degrees C
Term
What is the Km
Definition
Substrate concentrations that gives 50% of Vmax.
Term

What is one Kilocalorie?

 

Definition
The amount of heat energy needed to raise i kg or water i degree C at 15 degrees C
Term
What is direct calorimetry?
Definition
Measure of body's heat production to calculate energy expenditure
Term
What is indirect calorimety?
Definition
Calculates energy expenditure from respiratory ration RER
Term
What is the RER
Definition
VCO2/ VO2
Term

What is the RER value for rest?

 

Definition
0.70
Term
What is RER during maximal exercise?
Definition
Could be more than 1.0
Term
What is the relationship between myocardial stretch and stroke volume known as?
Definition
Frank-Starling mechanism
Term
What is used to produce ATP with RER closer to 1?
Definition
CHO and glucose
Term
When RER is 1.0, the consumption of 1 L of O2 releases how many kcal?
Definition
5.0 kcal
Term
Where is the measurement of RQ taken?
Definition
Muscle
Term
What is the food quotient equal to?
Definition
RQ= VCO2/O2
Term
What is the BMR
Definition
Minimum energy required for essential physiological function
Term
What is RMR
Definition
minimum energy required for normal daily activity
Term
From what three areas does total energy expenditure come from
Definition
Thermic effect of food (TEA), Resting metabolic rate, physical activity
Term
What is the general REE (RMR) in adults?
Definition
25-30 kcal/kg/24h
Term
Where is the most CHO stored?
Definition
Skeletal Muscle
Term
How many kcals are stored in skeletal muscles
Definition
~1600kcal
Term
What are the main fuels of oxidative metabolism?
Definition
Pyruvate and lactate
Term

What does the presence of glucose in adipose cells do?

 

Definition
Stimulates fat synthesis
Term
What is the recommended daily allowance for dietary cho?
Definition
130g/day
Term
What happens to pyruvate no immediately entering mitochondria?
Definition
reduced to lactate
Term
What do FADH and NADH do?
Definition
Function to consece and transport reducing equivalents within a cell
Term
What does NADH do what glycolysis procees slowly?
Definition
Tansports hydrogen and electrons to mitochondria.
Term
How many moles of ATP are produced from on mole of glycogen?
Definition
3 mole ATP
Term
How many moles of ATP are formed from one mole of glucose?
Definition
2 moles ATP
Term
What happens when too much pyrucvate is present?
Definition
Pyruvate is turned to lactate via LDH
Term
Lactate is major fuel for?
Definition
The heart
Term
what is the pKa or lactic acid?
Definition
3.87
Term
What is the pH of lactate?
Definition
7.3
Term
What happens when G6P levles increase?
Definition
Glycolysis is stimulated
Term
How can G6P be increased?
Definition
Stimulation of glycogenolysis by epine[hrine and contractions, and glucose uptake.
Term
Where does feedback control uauLLT EWAISW?
Definition
PFK to speed or slow regulatory enzymes
Term
When glycolysis is slow, LDH is in comperition with mitochondria for what?
Definition
Pyruvate
Term
Where does lactate oxidation take place?
Definition
Mitochondria
Term
Cori cycle pathway
Definition
muscle glycogen->muscle lactate->blood lactate->liver glucose ->muscle glycogen
Term
What does the addition of 2 hydrogen atoms to pyruvate form?
Definition
Lactage and NAD
Term
What is the function of NAD and FAD?
Definition
These are to transport electrons through the electron transport chain
Term
Which part of the mitochondria contains exchange enzymes for transport?
Definition
Innermembrane
Term
Where in the mitochondria are the LDH and kreb cycle enzymes?
Definition
The matrix
Term
What occurs in the cristae of the mitochondria?
Definition
oxidative phosphorylation
Term
Where is carnitine transferase found?
Definition
The innter wall of the mitochondria
Term
NADH is equivalent to how many ATPs
Definition
3
Term
Each FADH is equal to how many ATPs
Definition
2
Term
Where is the electron transport chain located?
Definition
inner mitochondrial membrane
Term
For each NADH entering the ETC, how many protons are pumped out?
Definition
6
Term
What happens outside the mitochondrian during ETC
Definition
A region of decreased pH and positive charge is created.
Term
Order of ETC
Definition
NADH dehydrogenase complex->ubiquinone ->Cytochrome b-c->ctochrome c-> cytochrome oxidase complex -> atpase
Term
How is pyruvate converted to ACoA?
Definition
PDH
Term
What are the products of the Kreb cycle?
Definition
ATP, CO2, and Hydrogen
Term
What is the making of triglycerides
Definition
Esterification
Term
What is the esterification
Definition
attachment of fatty acids to a glycerol by means of o2
Term
What enzyme is required for esterification?
Definition
Glycerol kinase
Term
Mobilization of fat is called?
Definition
Lipolysis
Term
What is used to break down tryglycerides in adipose tissue
Definition
ATGL
Term
How many carbons are lost in each cycle of the B-Oxidation pathway
Definition
2
Term
How many carbons are in each acetyl CoA
Definition
2
Term
What is PFK inhibited by?
Definition
ATP and Citrate
Term
Citrate is a byproduct of?
Definition
B-oxidation
Term
High production of Acetyl CoA implies what?
Definition
Diebetes
Term
recomended protein
Definition
0.8 mg/kg/d
Term
deamination is
Definition
removal of amion froud from a molecule taking place in the liver
Term
What is work due to preload called?
Definition
Flow work
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