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Chemicals used for the matinence of life, growth and reproduction of microbes. |
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Media which only contains inanimate materials. Used for Bacteria and Fungi. |
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Bacteria and Fungi use what kind of media? |
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Media which living cells (chick embryo,live animal or tissue culture use to live) Used for viruses and Ricikettsia. |
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What media type is used for Viruses and Ricikettsia? |
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What is the universal biological solvent in media? |
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What nutrient compounds are used in all media? |
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What are the inorganic nutrients? |
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Co2 H2S,NH3 , Phosphates, and sulfates |
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What are the organic nutrients? |
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Sugars, Fatty Acids,Amino Acids, Nucleic Acids. |
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Essential chemicals which cannot be made by bacteria but are critical to metabolism. |
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Compounds containing essential elements (C,O,N,S,H,P) which are oxidized to generate energy (ATP) or reduces to build cellular structures. |
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Inorganic G-F Growth Factors |
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Ca+,Mg++,Si++,Fe++,Co,Na+,K+ |
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Organic G-F Growth Factors |
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Vitamins and certain Amino Acids. |
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Bacteria Require a ________pH. |
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Fungi prefer an _________pH. |
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Media in which the exact chemical formulation is known. |
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not all chemicals are known(blood, tissues etc.) |
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purposes of various ingridents to promote growth |
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allows a large number of bacteria to grow. |
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Colony Morphology on agar gives clues to ID |
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Only Allows certain bacteria or fungi to grow ( dyes,pH) |
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Temporary holding media-preserves bacteria for lab. |
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Ingredients of Nutrient Broth and Nutrient Agar |
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1. H2O as biological solvent 2. peptone-protein digest which provides nutrients 3. Beef extract:provides groth factors 4. NaCl:to adjust Osmotic Pressure 5. Agar as solidifying agent. |
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Definition
Additives:Serum,yeast extract,blood,liver...etc |
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Indicators/dyes used to ID the pH and changes to pH as bacteria metabolize specific media(bromthymol Blue, Phenol Red, Methyl Red.) |
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Forms of Media Used in Micro 33 |
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Definition
1. broths 2. Slants 3. Plates 4. Fermentation Tubes 5. FTM-Thioglycollate Broth (O2 requirements) 6. stabs |
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