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MCB Exam 1
Exam 1
50
Microbiology
Undergraduate 2
09/14/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Microbe
Definition
Simple structures; Most abundant organism on Earth
Term
Prokaryotic cells
Definition
Lack a membrane bound nucleus
Term
Eukaryotic cells
Definition
Have more complex, membrane bound organelles
Term
Carl Woese
Definition
Made 3 domain system in 1970's based on RNA: Eukarya, Bacteria, Archaea
Term
Domain Bacteria
Definition
Usually single celled; cell wall of peptidoglycan; considered prokaryotes; some cause disease but most are beneficial and recycle elements
Term
Domain Archaea
Definition
lack peptidoglycan in cell walls; many live in extreme environments; role in disease is unclear; some have unusual metabolic characterizations (ex. methanogens produce methane gas)
Term
Domain Eukarya
Definition
Usually larger than Bacteria and Archaea; Ex. fungi, protozoan (grazers), animals, plants, slime molds, water molds (protists that grow in moist places), and algae
Term
Viruses
Definition
Simple, most are just protein & nucleic acid; requires host to replicate; NOT PART OF 3 DOMAINS b/c it has no RNA
Term
Viroids and Virusoids
Definition
Infections agents made of RNA
Term
Prions
Definition
Infectious proteins (Causes diseases like mad cow disease)
Term
Definition of life
Definition
-reproduction
-energy use
-have internal order
-growth and development
-maintain homeostasis
-response to environmental changes
Term
Ribozymes
Definition
Form peptide bonds and do cellular work; discovered by Thomas Cech in 1981
Term
Earliest Metabolism used..
Definition
1. inorganic materials for energy
2. photosynthesis (cyanobacteria release oxygen)
Term
How did cellular microbes evolve
Definition
1. Eukaryotes sexually reproduced
2. Archaea and Bacteria horizontally exchanged genetic info
3. Mutations led to new genes
Term
Leeuwenhoek
Definition
Made lens out of glass; first person to accurately describe microorganisms; studied diarrhea and teeth scrapings
Term
Francisco Redi
Definition
disproved spontaneous generation with meat covered with paper experiment
Term
John Needham
Definition
Boiled then sealed mutton broth; didn't realize microorganisms could settle from air
Term
Lazzaro Spallanzani
Definition
Sealed then boiled mutton broth; no microorganisms grew because they were killed in boiling (after Needham)
Term
Louis Pasteur
Definition
SETTLED THE MATTER OF SPONTANEOUS GENERATION by using curved neck flasks; developed pasteurization; microorganisms ferment
Term
John Tyndall
Definition
Proved dust carried microorganisms (after Pasteur)
Term
Ferdinand Cohn
Definition
Heat resistant bacteria can produce endospores
Term
Bassi, Berkley, Bary
Definition
Bassi- showed silkworm disease was from fungi, Berkeley- Potato famine was caused by water mold, fungi causes cereal crop disease
Term
Ignaz Semmelweis
Definition
Realized high mortality rate at hospital was from people not washing hands after being in autopsy room
Term
Joseph Lister
Definition
Figured out to clean instruments and wounds with phenol
Term
John Snow
Definition
1854 Cholera outbreak in London--500 died in 10 days, figured out it was from sewer leaking into water well
Term
Robert Koch
Definition
Established relationship between bacillus anthracis and anthrax; developed Koch's postulates (links disease to microorganisms)
Term
Koch's postulates
Definition
1. microorganism must be present in sick person and absent in healthy person
2.Suspected organism must be isolated and grown as pure culture
3. Same disease must occur in healthy host
4. Organism must be isolated again from diseased host
Term
Edward Jenner
Definition
Used vaccine procedure to protect from small pox (saved 8 year old boy); Before Pasteur!!
Term
Paul Ehrlich
Definition
1910: First chemotherapy; Salvation from syphilis
Term
Alexander Fleming
Definition
1928: Discovered first antibiotic-observed penicillin fungus killed S. aureus; 1940's: Penicillin is mass produced
Term
Basic vs. Applied Aspects
Definition
Basic- individual groups of microbes, genetics, etc.
Applied- practical problems like disease, water, food
Term
Resolution
Definition
Ability to distinguish differences between things that are close together (Resolution of 0.2 microns means you can see between things that are 0.2 apart)
Term
Numerical Aperture
Definition
Ability of lens to gather light (depends on refractive index of medium, increases as R.I. increases)
Term
Commonly used Flourochromes
Definition
1.Acridine orange and DAPI stain DNA
2. TRITC and FITC attach to antibodies that bind to cellular components (rabbit example to find disease)
Term
Heat Fixation
Definition
Routinely used with bacteria and archaea; gives you overall morphology but inactivates enzymes/proteins
Term
Chemical Fixation
Definition
Used with larger organisms; immobilizes proteins, lipids but protects delicate cell substructure (Ex. Formaldehyde)
Term
Simple staining
Definition
Uses dyes to increase visibility and contrast (allows shape size arrangement to be seen); use chromophore groups (have double bonds) to bind cells and give color
Term
Basic dyes
Definition
Positively charged bind to negatively charged molecules (proteins, nucleic acids) Ex. safranin, crystal violet
Term
Acid Dye
Definition
Negatively charged so binds to positively charged structures and are repelled by cell surface; produces negative stain, good for seeing capsules. Ex. eosin
Term
Differential staining
Definition
Divides microorganisms into groups based on properties; allows presence/absense of structures to be seen (flagella, endospores)
Term
Christian Gram
Definition
1884 he divides BACTERIA into two groups (Gram positive and Gram negative)
Term
Gram positive
Definition
Thick wall of peptidoglycan; holds in purple color after staining
Term
Gram negative
Definition
thin wall of peptidoglycan; only holds Safranin counterstain and is therefore pink
Term
Acid fast staining
Definition
Uses heat and phenol to drive fuschion into cells that dont Gram stain (like Mycobacterium tuberculosis and M.leprae) because of their lipid cell wall
Term
Endospore staining
Definition
Uses high heat and double staining to make green endospores on pink cells (Used on stain resistant micro's like Bacillus and Clostridium)
Term
Flagella staining
Definition
Can only be seen in electron microscope; uses mordant and stain to increase size of flagella
Term
Why do electron microscopes have better resolution than light microscopes?
Definition
Electrons have a shorter wavelength than light
Term
Electron cryotomography
Definition
Created in 1990's to freeze native state of organism in vacuum and create 3d images by putting together many different angled pictures (used on cytoskeletal structures, flaggelar motors, etc)
Term
Macroelements
Definition
Needed in large amounts
1. C,O,P,S,N,H - found in organic molecules like proteins, nucleic acids
2. Ca,Mg,K,Fe - exist as cations and responsible for enzyme activity and biosynthesis of ATP by electron transport
Term
Makeup of Important Cell Molecules (4)
Definition
1. Proteins- CHONS
2. Nucleic acids- CHONP
3. Lipids- CHOP
4. Carbohydrates- CHO
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