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LLUSD Microbio sec 2 exam
Dr. Johnson sec 2 exam , LLU School of Dentistry microbio
104
Microbiology
Graduate
05/27/2014

Additional Microbiology Flashcards

 


 

Cards

Term
Serpentine Cords

High lipid content in cell wall
Definition
Mycobacterium Tuberculosis
Inhalation
Acid Fast bacteria that also have Wax D (induce delayed hypersensitive rxn) and Cord Factor
Causes Pulmonary TB + Miliary TB
Triple therapy - LONG TERM - INH + rifampin Pyranzinamide
Term
Hansens Disease
Definition
Mycobacterium leprae
Leprosy. . . treat with Dapasone
inhalation
Likes to infect nerves
Term
White type of Leprosy can cause false + syphilis ?
Definition
Lepromatous

Tuberculoid does not , usually self limiting
Term
Most common cause of Human pneumonia
Definition
Streptococcus Pneumoniae
polysaccharide capsule
normal flora. . . inhalation
classical lobar pneumonia
Penicillin treatment
vaccine - Conjugated Prevnar (kids 2 and younger) ; Multivalent Pneumovax (adults)
Term
whooping cough
Definition
Bordetella Pertussis
Virulent strains are encapsulated and toxin producing
inhalation
URT and LRT , paralysis of ciliated cells
Vaccine - TDaP
Term
Secretes proteases which destroy cellular proteins
spread through fine water mist
Definition
Legionella pneumophilia
intracellular; motile; Legionnaires Disease
URT illness
Levofloxacin + Azithromycin
Term
Metachromatic granules and Pseudomembrane formation in URT
Definition
Corynebacterium Diptheriae
Gram + ; "chinese characters"
Diptheria
Exotoxin (due to TOX gene bacteriophage transduction)
inactivates EF2
rash indistinguishable from impetigo
normal flora
Vaccine - TDaP
Term
Catalase + with Polyribophosphate capsule, X factor, V-factor
Definition
Haemophelius (influenzae)
requires blood components for growth
X factor - hemin
V-vactor - B complex vitamin
Hib Vaccine
Term
"currant jelly"
Definition
Klebsiella pneumoniae
enteric organism ; large gelatinous capsule
Intestinal and respiratory tract
2 species K.ozenae and K.rhinoscleromatis
Term
Neonatal Pneumonia and/or Meningitis
Definition
Listeria monocytogenes
crosses placental and affects fetus
Intracelular
Psychrophilic
Dairy product transmission (Brucellae, Coxiella brunii, Y.enterocolitica)
Amoxicillin, Vancomycin, Ampicillin
Term
inhalation of dried bird feces
Definition
Chlamydia psitticosis
atypical pneumonia resembling influenza***
No vaccine
Antibiotics
Term
No cell wall ; requires cholesterol to grow ; Walking Pneumonia
Definition

Mycoplasma not effected by penicillin ; use tetracycline ; no vaccine

Obligate Intracellular

Term
Actinomyces israelii
Definition
Sulfur granules
"Lumpy Jaw"
Normal Flora ; endogenous to the mouth
Penicillin or Tetracycline
Anaerobic
Term
2 acid fast (Zeil-Nelson stain) bacteria
Definition
Mycobacterium
and
Nocardia asterodies (pulmonary and CNS associated)
Term
Gram + bacteria
Definition
Actinomyces
Bacillus
Corenybacterium
Clostridium
Listeria
Streptococcus
Staphylococcus
Term
Relapsing Fever
Definition
Borrellia (spirocheate)
also can cause Berreliosis and Lymes Disease
Vector mediated
Term
Vincents Angina
Definition
Borrelia Vencetti
causes ANUG - acute necrotizing ulcerative gingivitis
Term
Louse Borne disease
Definition
Borrellia recurrentis - relapsing fever
Ricketsiae Prowazakii - Epidemic typhus
- obligate intracellular
Term
hard shell tick vector
Definition
Borrelia Burgdorferia - lymes disease
Ricketsiae rickettseii - **Rocky Mountain Spotted Fever**
- obligate intracellular
Term
Endemic Relapsing Fever
Definition
Borrelia duodenii
soft-shelled tick borne
warm weather
multiple dose of tetracycline or erythromycin
animal-human transmission via saliva of ticks
Term
Most common vector of tick (vector) borne disease in the USA
Definition
Borrelia burgdorferia
Lyme's Disease
Hard shelled Tick
Erythma migrans - Bulls eye target rash
CNS manifestations w/ AV block and subtle myocarditis and arthritis
NO human to human
Reservoirs : Rodents Deer, Domestic pets
multiplel doses of oral Doxycycline
Term
Obligate intracellular organisms
Definition
Chlamydia
Ricketsiae - ***except R.quintana = extracellular
Mycoplasma
Term
Jaundice ; Weil's Disease ; Canicola fever
Definition
Leptospira interogans
Leptospirosis - pathongenic
Canicola - dog MOST COMMON in USA
Contact with ANIMAL URINE. . . break in skin or inhalation
Systemic disease involves. . . Brain, Lungs, Liver, Kidney
(colonize)
IV penicillin, YES vaccine
Term
Brucellae
Definition
Undulating fever
LPS endotoxin
Intracellular
B.melitenisis - goats&sheep , most pathogenic
Localized to mammary glands & preg. uterus
Skin abrasions, conjunctival exp, inhalation
MILK UNPASTURIZED ! ( Y.enterolitica, Listeria, Coxiella bruneii)
LONG LONG recovery weeks to months
Term
Coagulase produced at 28C, Rat flea vector
Definition
Yersina Pestis / Enterolitica
Fraction I - antiphagocitic
Black death, bubonic/pneumonic plegue
LPS and Murine Exotoxin
Optimum temp = 30C , envelope slime layer @37C
Y.e. - milk - fever, bloody diarrhea - Resembles APPENDICITS
Y.p - Rat flea - large painful buboes, vomit , diarrhea
Term
Typhoidal and Pneumotitis Tuleremia
Definition
Francicella
TT - most serious - ingestion
PT - most frequent - systemic
JACK RABBITS !
Intracellular
Term
Normal flora
Definition
*Pasturella multocida - most common organism from infected dog & cat bites
*Actinomyces - sulfer granules
*Streptococcus pneumoniae - most common pneumonia
*Corenybacterium diptheriae - metachromatic g. pseudom.
*Malisazia furfur (fungus - superficial mycoses) - UV green
Term
Ricketsiae
Definition
Obligate intracellular
*except for R.Quintana = extracellular
Term
Epidemic Typhus
Definition
R.prowazekii
human body louse vector - similar to B.recurrentis
gangrene in severe froms
highest fatality rate = 10-40%
Yes vaccine
Term
Rat flea / rat louse
Definition
Rat flea - Y. pestis - black death, bubonic/pneumonic plague
Rate Louse - R.typhi - Endemic typhus
Term
Rocky Mountain Spotted Fever
Definition
Ricketsiae Rickettsii
Hard shelled tick - *similar to B.burgdorferia (lyme's dis)
intravascular coagulation in severe cases
Term
Q Fever
Definition
Coxiella brunii
infected milk, inhalation (urine, feces, animal hides)
Vector - arthropods , especially ticks
possible GI symptoms and chest pain
Doxycycline
Vaccine- QVAX
Term
Cancer causing viruses
Definition
HPV --> Oropharyngeal + cervical (16+18)
Hepatitis B (HBV) --> (see below)
Hepatitis C (HCV) --> hepatic cell carcinoma
Epstein-Barr Virus (HHV4) --> Nasopharngeal + burkitts lym
Term
Lipid Envelope with E1 and E2 viral glycoproteins
Definition
Coronavirus
+ssRNA, long helical structure
Inhalation
Respiratory Disease (URT) - NO FEVER
Gastrointestinal disease
growth cycle in cytoplasm
Term
Picornaviruses include
Definition
Polivirus - anterior horn cells of spinal cord*** & asp meng
Coxsackie (A+B) virus - A - herpangina
Echovirus -
Rhinovirus - ONLY one that is inhalation , common cold
Hepatitis A - jaudice
Term
General Picornavirus traits
Definition
+ssRNA
No envelope
Icosahedral
Replicate in cytoplasm
Stable at pH 3-5
Term
anterior horn cells of spinal cord
and possible
Viral (aseptic) meningitis
Definition
Poliovirus
ingestion - for all picornaviruses EXCEPT for rhinovirus
+ssRNA
No envelope , icosahedral
Replicate in cytoplasm
Progressive postpoliomyelitis muscle atrophy
Vaccine: Salk (killed) , Sabin (live attenuated, BEST ONE)
Term
Lesions in CNS , heart muscle, other organs
Neonatal disease, acute fatal encephalomyocarditis (infants) and acute myocardiopathy
Definition
Coxsackie B virus
Coxsackie A virus --> herpangina
BOTH grow in oropharnx and intestine
Ingestion
CNS and peripheral nerve involvement
NEVER treat with Asprin --> Reyes syndrome
Term
Reyes Syndrome
Definition
Coxsackie virus
Influenza Virus
Human Herpes Virus 3 - VZV

DO NOT TREAT WITH ASPRIN
Term
Only picornavirus that is transmitted via inhalation , Common cold
Definition
Rhinovirus
Acid laible
Heat stable (grow best at 33C)
NO FEVER ***same as Coronavirus
Term
6 characteristics of a virus
Definition
Very small
DNA or RNA (NOT BOTH)
Obligate intracellular parasite
No ribosomes
Not susceptible to antibiotics
specific tissue tropism
Term
4 ways of classification of viruses
Definition
Symmetry
Nucleic Acid type
Host cell type
Serological relationships
Term
5 Enteroviruses of Picornaviruses
Definition
Poliovirus
Coxsackie A virus
Coxsackie B virus
Echovirus
Enterovirus
Term
True or False
+ssRNA can create viral proteins immediately
Definition
True
-ssRNA cannot, they need to make the template strand first.
Term
Name the DNA viruses
Definition
HPV - circular DNA
Hepatitis B - dsDNA
Human Herpes Virus 1-8 - dsDNA
Term
Which of the following does not belong to the family Paramyxovirus?
Parainfluenza, Respiratory Syncytial Virus, Measles, Mumps, Rubella
Definition
Rubella does NOT below to paramyxoviruses. it is a Togavirus
+ssRNA ; Icosahedral
Inhalation
Fetus malformation + mild upper respiratory symptoms
YES vaccine - MMR (live attenuated)
Term
which group of viruses has Hemagluttin-neuramidase and fusion glycoproteins ?
Definition

Paramyxoviruses

including (Measles, Mumps, RSV)

Term
What is the most important cause of lower respiratory tract illness in infants especially between 2-4months old ?
Definition
Respiratory Syncytial Virus (RSV)
it is a paramyxovirus
- linear RNA
helical nucleoprotein capsid
immediate hypersensitivity (type I - IgE) rxn
Treat with Ribavirin
Term
Generalized exanthematous (skin eruption) disease with characteristic Koplik spots on buccal mucosa.
Definition
Measles
inhalation
Multinucleated giant cells - *similar to RSV syncitia
reticuloendothelial system and Rash w/ possible CNS
Prodromal fever
Vaccine yes - MMR
Term
Killed vaccine which has no fusion proteins gives incomplete protection against ?
Definition
Measles
Term
3 Key things about Mumps
Definition
1. Parotitis & salivary gland infection
2. Orchitis (testes/ovaries)
3. Aseptic meningitis (* similar to poliovirus)
Term
Non-enveloped virus/es
Definition
HPV
Picornaviruses
Term
HPV 16 and 18 are known causative agents of what type of cancer?
Definition
Cervical cancer

HPV 6 - Genital warts (condylomas) - most common STD
HPV 1-4 - cutaneous warts
Term
Gardasil and Ceravix are used to treat what ?
Definition
Gardisil - HPV 6, 11, 16, 18 serotpyes - prevent warts and protect against cervical cancer.

Ceravix - HPV 16 and 18 serotypes protect ONLY against cervical cancer
Term
Condoms can reduce risk of what virus ? but not another ?
Definition
Condoms can reduce risk of HIV but NOT HPV
Term
What virus takes residence in the basal cell layer of squamous epithelial cells, but does not lyse them ?
Definition
HPV
Term
Hemagglutinin (HA) and Neuraminidase (NA) are matrix proteins of what virus ? while hemagglutinin-neuraminidase (HN) is on the envelope of which virus ?
Definition
Q1: Influenza Virus - have HA and NA
Q2: Paramyxoviruses (RSV, Measles, Mumps) - has HN

HA - helps with attachment to sialic from host cell for virus fusion
NA - lowers the viscosity of the host cell membrane prior to vision release from cell.
Term
which two virus undergo massive amounts of genetic reassortment/change ? which of the two has a higher mutation rate ?
Definition
Influenza - changes yearly (genetic shift and/or drift)
HIV - changes multiple times within an infected individual
*HIV has a much higher mutation rate.
Term
Myxoviruses have an affinity for what type of environment ?
Definition
Mucous
Term
amantadine and rimantidine are used to treat what ?
Definition
Influenza A virus
NOT effective against H5N1 or avian flu viruses.

Vaccines for influenza are typically Tri/tetravalent with several strains of A and B types
Term
Lipid enveloped virus is characteristic of what group ? are they DNA or RNA viruses ?
Definition
Orthomyxovirus (eg. Influenza virus) - -ssRNA
Herpesvirus (eg. HSV1 , VZV,) - dsDNA
HIV - 2 identical copies of linear ss RNA . . .needs Reverse Transcriptase
Term
Respiratory Papillomatosis is the result of ?
Definition
mother --> fetus viral transmission via aspiration of HPV during birth.
Term
what test detects for abnormalities of cells in the cervix of females ?
Definition
Papanicolaou (PAP) smear
Term
Which Hepatitis viruses are chronic ?
which hepatitis viruses requires HBV infection prior ?
Which hep. virus/es are sexual/blood transmitted ?
What is the most important factor for measuring the infectivity potential of a hepatitis virus ?
Definition
Q1: Hep B, C, and D
Q2: Hep D
Q3: Hep B, C, and D
Q4 : HBeAg
Term
what are 3 other viruses that can cause hepatitis ?
Definition
Epstein-Barr virus (human herpes virus 4)
Cytomegalovirus (human herpes virus 5)
Measles (rubeola)
Mumps
Rubella
Coxsackievirus
Term
to be categorized as chronic hepatitis what is the criteria ?

Which is the most chronic hepatitis ?
Definition
HBsAg must be in circulation for 6months or LONGER.

Hepatitis C is most chronic
Term
in the early acute phase of Hepatitis A virus (HAV) what is present ? what about in the acute phase ? convalescent phase ?
Definition
anti-HAV IgM
anti-HAV IgM and anti-HAV IgG
anti- HAV IgG
Term
an individual is contagious with Hepatitis B as long as what 2 things are in circulation ? which of those would be absent during the convalescent phase "window phase" of an HBV infection?
Definition
HBsAg and HBeAg
during the convalescent phase, only HBeAg would exist. HBsAg would be ABSENT !
Term
which is more infective HIV or Hepatitis B ?
Definition
Hepatitis B
Term
name a specific virus that is a Flavivirus ? Calicivirus ?
Definition
Hepatitis C = Flavivirus
Hepatitis E = Calicivirus
Term
the most common human virus is ?
Definition
Herpesvirus
Term
3 important life cycle growth traits of all herpes viruses are ?
Definition
1. "ballooning of cells"
2. intranucleuated inclusion bodies
3. multinucleate giant cells
Term
HSV1 commonly affects what area of the body ? where does it remain latent ? mode of transmission ? 2 characterisitc clinical findings include ?
Definition
Q1: oral cavity (lips)
Q2: Trigeminal Ganglia
Q3: inhalation via respiratory droplets OR direct contact
Q4: Whitlow's infection (fingers) and Eczema herpeticum
Term
HSV2 primarily affects what part of the body ? mode of transmission ? site of latency ? what clinical manifestations ?
Definition
Q1 : Genitals
Q2 : sexual OR mother--> fetus
Q3 : Sacral ganglia
Q4 : Genital herpes, neonatal herpes, and Whitlow's inf. + Eczema herpeticum
Term
What is the drug treatment for HSV1 and 2 ? what does it do ?
Definition
Acyclivor , nucleosides get phosphorylated by Thymidine kinase and DNA replication is blocked.
Term
What is Human Herpes Virus 3 ?
Definition
Varicella-Zoster Virus = chickenpox and shingles
you cannot get shingles if you have not had chickenpox
Varicella - mild, generalized rash, children usually
Zoster - unilateral rash (follows nerve tracts), extremely painful,
Term
infectious Mononucleosis refers to what virus ?
Definition
Epstein-Barr virus (Human Herpes Virus 4)
"Kissing Disease" - saliva
immortalizes B cells, and remains latent in B cells
Atypical lymphocytes and HETEROPHILE ANTIBODIES !
Nasopharyngeal carcinoma and Burkitt's Lymphoma
Term
what virus is called the salivary gland virus and exhibits cell gigantism, but has NO heterophile antibodies ?
Definition
Cytomegalovirus
Latent in secretory glands and Kidneys
Term
Human Herpes Virus 6 and 7 have genomic similarity with ? but share ?
Definition
genomic similarity with Cytomegalovirus
they share antigenic similarity

BOTH grow in T lymphocytes
Term
Kaposi's sarcoma is indicative of what 2 viruses ?
Definition
Human Herpes Virus 8
and
HIV/AIDS
Term
what functions belong with head of the molecules. . .
gp120 , gp41, CXCR4, CCR5
Where virus are these important for ?
Definition
gp120 - virus attachment to CD4 celles
gp41 - fusion
CXCR4 - T cell entry
CCR5 - macrophage/monocyte entry
HIV VIRUS !
Term
What two viral infections can induce Fc receptors that help predispose an individual to contracting AIDS virus ?
Definition
HSV and CMV induce Fc receptors . . . these can be later used by the HIV virus-antibody complex to enter cells.

*NOTICE, both of these are Herpes Virus !
Term
What is the hallmark for an AIDS virus infection ?

what is the major reservoir of the AIDS virus ?
Definition
T helper lymphocytes (CD4) get infected, easily killed, allow many progeny viruses, and form syncytia.

major reservoir is the Moncytes and macrophages
Term
What is the best predictor of AIDS virus outcome ?
Definition
Level of HIV1 RNA in the plasma ("viral load")
Term
What are 2 (3?)AIDS defining conditions ? What is the recommended treatment for HIV/AIDS ?
Definition

Q1 : Pneumocystis Carinii (Jirovecii) peumonia (PCP) Kaposi's Sarcoma

Histoplasma Capsulatum 

 

Q2 : Highly Active Anti-Retroviaral Therapy (HAART) , it is a 3 drug combination (AZT, 3TC, and Indinavir). AZT targets Reverse transcriptase. Indinavir targets protease inhibitors. Prevention of envelop fusion with host cell membrane

Term
what is the screening test for HIV/AIDS ? what is the confirmatory test ?

if you are HIV positive during the screening test do you have HIV/AIDS ?
Definition
ELISA = screening test
Western Blot = confirmatory test

Just because you are HIV+ in the ELISA screening test DOES NOT mean you have HIV/AIDS it is a possible false +
Term
What are the 3 dermatophytes ? which causes athletes foot ? which causes jock itch ?
Definition
Microsporium
Trichophyton - athletes foot
Epidermophyton floccosum - jock itch
Term

[image]is this macro or microconidia ? which family does it belong to ?

Definition

Macroconidia - large spindle shaped spines

 

Dermatophyte (sueprficial mycoses) - Microsporium 

 

UV light fluorescence blue green 

 

Treat with Allylamines (eg. Lamisil) and Azoles . . . BOTH target the sterols of the membrane

Term

[image]Micro or macroconidia ? which family do they belong to ?

Definition

Macroconidia - club shaped

Dermatophytes (superfical mycoses) - Epidermophyton f. 

 

tinea cruris (groin) , pedis (foot), and unguium (nail)

Term

What is the causative agent of a superfical infection of the Stratum corneum ? 

 

A sample taken from the infection fluoresces green.[image]

Definition

Malasazia furfur (lipophilic fungus)

"spaghetti and meatballs"

Normal flora . . .the ONLY other commensal along with Candida 

 

causes Tinea Versicolor 

Term

Not a dimorphic fungus 

 

what is its tropism ?

[image]

Definition

Cryptococcus neoformans (systemic mycoses)

budding yeast organism

Polysaccharide capsule

Urease Positive 

inhalation . . . high risk groups are immunocompromised

LUNG TISSUE infected 

Tropism : Neurotropic for CNS --> Brain Abscess + Meningitis 

Histological presentation - "fried egg" in brain tissue

best demonstrated with INDIA INK

 

Term

has single buding yeast cells with Figure 8 nucleus. . .

treated with Oral Potassium Iodide (simple method)

[image]

slide 34

Definition

Blastomyces dermatitidis

Inhalation . . . soil + birds

LUNG TISSUE

Most effective treatment is with Amphotericin B, Azoles, and antifungals

Term

What is this ? name of fungus ? Characteristic trait of yeast cells ?

[image]

slide 39

Definition

Paracoccidiodes (blastomyces) brasilienis

multiple-buding yeast cells

has estrogen receptors preventing yeast formation 

Associated with BATS and ARMADILLOS

Inhalation via Soil + plants

infects mucous membranes of nose, mouth, anus

IMPORTANT DIAGNOSTIC - development of multiple daughter cells + multiple budding yeast forms (see picture)

 

Term
What is common to all the systemic mycoses ?
Definition

They all . . .

  • are found environmentally related to soil
  • are transmitted by Inhalation
  • infect LUNG TISSUE
  • can cause mucous-cutanous lesions under appropriate conditions , pulmonary lesions, dissemination

 

Term

What is diagnostic characterisitic of Histoplasma Capsulatum?

[image]

slide 41

Definition

DIAGNOSTIC = Tuberculated Chamydospores 

Intracellular 

spores are inhaled

LUNG TISSUE 

in an HIV+ patient , considered an "AIDS defining illness"

"flu like" symptoms that can be confused with Miliary TB

can lead to Hepatosplenomegaly, leukopenia, and thrombocytopenia. 

TREAT with Oral itraconazole

 

 

Term

What is fungus is this ?

[image]

slide 43

Definition

Histoplasma capsulatum 

 

intracellular Tuberculated chlamydospores can be seen 

Term

What is this fungi ?

[image]

Definition

Coccidiodies immitis . . . dimporphic

  • arthrospores (arthroconidia) alteranating filled an unfilled
  • endosporulating spherules in infected tissue
  • valley fever
  • Inhalation 
  • LUNG TISSUE
  • Treat with Amphotericin B and/or Ketoconazole
Term

What is this fungi ?

[image]

slide 47

Definition

Coccidiodies immitis

 

Endosporulating spherules 

Term
Which of the systemic mycoses can be chronic ?
Definition

Histoplasma capsulatum 

 

and

 

Coccidiodies immitis

Term
which opportunistic fungi is NOT thermodimorphic ?
Definition

Candida

  • they can exist as yeast or mold regardless of temp.
  • cause...oral thrush, cutaneous candidiasis, intestinal candidiasis, candida vaginitis , and systemic candidasis. 
  • Endogenous to mouth, surface of skin, intestine, vagina
  • Broad-spec antibiotics, cortisone, anethesia, change in pH are all predisposing factors
  • Topical Nystatin or amphotericin B - for treatment 
  • Ketoconazole for systemic infection
Term

Penicillin producing ?

[image]

Definition

Aspergillus penicillium 

 

exogenous species 

 

opportunistic

 

lung tissue and invasion of blood vessel walls surrouunding tissue. 

Term
[image]
Definition
Candidiasis Albicans
Term

Once considered a protozoa , but now known as a fungus. 

It is insensitive to Amphotericin B.

[image]

Definition

Pneumocystis Carinii 

 

  • "deflated ball" shape
  • lacks erosterol , so CANNOT use amphotericin or azole drug treatments
  • almost EXCLUSIVELY seen in AIDS pts. (PCP)
  • Trimethoprim-sulfamethoxazole (bactrium) - treat
  • pentamindine (more toxic) - treatment
  • SILVER STAIN of lung biopsy for yeat organisms (cysts)*
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