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Microbiology Specimen Management
Media & Bacterial Identification Methods
16
Microbiology
Graduate
10/18/2012

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Cards

Term
Anaerobic specimens
Definition
should NOT be refrigerated if processing cannot be done immediately because refrigeration can oxygenate the specimen and potentially kill it
Term
Anticoagulant for blood cultures
Definition
SPS (sodium polyanethol sulfonate) at a concentration of 0.025%
Term
Least desirable specimen
Definition
Swab because better specimens are usually available
Term
Holding a urine specimen
Definition
at 4  C while waiting for transport to the laboratory to halt microorganisms growth and maintain the microorganisms already in the sample
Term
Reasons to reject a specimen
Definition
Collected after antibiotics were given
Term
Turbidity
Definition
cloudiness
Term
Selective media
Definition
Media that inhbitis the growth of some organisms
Term
Differential media
Definition
Media that contains factors used to distinguish characteristics
Term
Enriched media
Definition
Media that contains nutrients required for growth
Term
Microaerophilic
Definition
requires low levels of oxygen
Term
Capnophilic
Definition
grows best in 5-10% carbon dioxide
Term
Facultative anaerobe
Definition
Can grow aerobically or anaerobically
Term
Hemolysis
Definition
lysis of red blood cells
Term
What do dark pink colonies on MacConkey agar indicate?
Definition
the presence of lactose fermentors (salmonella or shigella from stool sample)
Term
How would you describe colonies produced on MacConkey agar by nonfermenting gram- bacteria?
Definition
Colorless
Term
Differentiate Beta Hemolysis from alpha hemolysis
Definition
Beta Hemolysis is complete in that there is a clearing of the colored agar around the colony, while alpha hemolysis is not complete and there is green color around the edges of the colony
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