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Lecture 8
Important Terms
26
Biology
Undergraduate 2
10/09/2011

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Term
Prokaryote
Definition
Any organism whose genetic material is not located within a membrane-bound nucleus
Term
Eukaryote
Definition
Any organism whose cells have a membrane-bound nucleus in which the genetic material is located and membrane-bound organelles
Term
Plasmid
Definition
An extrachromosomal, double-stranded DNA molecule that replicates autonomously from the host chromosome
Term
Episome
Definition
In bacteria, a plasmid that is capable of integrating into the host cell's chromosome
Term
Cotransduction
Definition
The simultaneous transduction of two or more bacterial genes, a good indication that the bacterial genes are closely linked
Term
Haploid
Definition
A cell or an individual with one copy of each nuclear chromosome
Term
Bacteriophage
Definition
Viruses that attack bacteria
Term
Prototroph
Definition
wild type, synthesize what they need for growth and reproduction
Term
Auxotroph
Definition
Mutant type, lack an enzyme necessary for growth
Term
Minimal Media
Definition
Only prototrophic bacteria can grow on this
Term
Complete Media
Definition
Contains all substances required by all bacteria, including auxotrophic bacteria
Term
Conjugation
Definition
Requires physical contact between two bacterial cells for DNA transfer, one-way transfer from donor to recipient, non-reciprocal
Term
Transformation
Definition
Donor cell DNA is lysed and the fragments are taken up by the recipient cells
Term
Transduction
Definition
Bacterial virus (phage) transfers the DNA from donor cell to recipient cell
Term
F factor (Fertility Factor)
Definition
Mediates conjugation by deciding which bacteria can conjugate through physical contact
Term
Hfr (High-Frequency Strains)
Definition
Donor cells with F factor integrated into the donor bacterial chromosome
Term
F+ Cells
Definition
Donor cells with F factor
Term
F- Cells
Definition
Recipient cells lacking F factor
Term
Merozygote
Definition
Partially diploid cells that contain 2 copies of some bacterial genes. Produced by a F- and F' cell
Term
F' Cells
Definition
Cells with F factor carrying bacterial genes
Term
Competent Cells
Definition
Cells that take up DNA in transformation
Term
Transformants
Definition
Cells that recieve genetic material in transformation
Term
Lysogenic Cycle
Definition
Phage integrates into bacterial chromosome and is inactive
Term
Lytic Cycle
Definition
The phage always lyses the host cell
Term
Cotransformed
Definition
Cells that are transformed by at least two genes
Term
Pilus
Definition
Used to nick F- cells and donate genetic material during conjugation
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