Term
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Definition
| describes the introduction of DNA not mammalian cells even though no viral nucleic acid is involved |
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Term
adding DNA with cationic carriers that bind to and protect DNA;
allowing DNA to be taken up by phagocytosis;
DNA injected directly into the nucleus |
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Definition
| How is transfection in mammalian cells accomplished? |
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Term
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Definition
vectors that can replicated and be stably maintained in 2 or more unrelated hosts
allows for movement of genes between unrelated organisms without modification of the vector |
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Term
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Definition
| allows for control of expression of clones gene, contains regulatory sequences for manipulation of expression |
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Term
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Definition
| expression vector developed to be stably maintained in host, maintained at low copy number |
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Term
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Definition
| infectious virus vector that replicates without killing its host |
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Term
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Definition
plasmid vectors with the cos site from the lambda genome
can be packaged into lambda virions, more stable than plasmids, |
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Term
| bacterial artificial chromosomes (BAC) |
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Definition
| vector based on F plasmid |
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Term
| Yeast artificial chromosomes (YACs) |
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Definition
| hold most DNA of any vector but has problems with rearrangement and recombination |
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