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Lecture 36
Brock
30
Microbiology
Undergraduate 3
04/24/2014

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Term
transformation
Definition
free DNA is taken up by another cell
Term
transduction
Definition
DNA transfer mediated by a virus
Term
conjugation
Definition
DNA transfer involves cell-to-cell contact and a conjugative plasmid in the donor cell
Term
recombination
Definition
physical exchange of DNA between genetic elements
Term
homologous recombination
Definition
genetic exchange between homologous DNA sequences (not quite the same but very similar) from two different sources)
exchanges a gene
Term
lateral gene transfer
Definition
adds gene (does not replace it)
Term
transformation
Definition
incorporation of free DNA into a recipient cell
Term
competent cells
Definition
bacteria and archaea that are naturally transformable
Term
transfection
Definition
incorporation of viral DNA
Term
electroporation
Definition
technique used to get DNA into organisms that are not readily transformable
Term
competence-specific protein
Definition
protein that binds to DNA (during/after transformation) once inside a cell to protect it from degradation
Term
RecA
Definition
protein that integrates DNA into host cell's chromosome during transformation
Term
generalized transduction
Definition
DNA from any portion of the host genome is packaged into the virion to be transferred to another cell
Term
specialized transduction
Definition
DNA from a specific region of the host chromosome is integrated directly into the viral genome (replacing viral genes)

usually makes virus non-infectious
Term
transducing particle
Definition
host DNA that is accidentally packaged into a virion in generalized transduction
Term
bacterial conjugation (mating)
Definition
mechanism of genetic transfer involving cell-to-cell contact
Term
donor
Definition
cell that contains conjugative plasmid in conjugation
Term
recipient
Definition
cell that does not have a conjugative plasmid in conjugation
Term
sex pilus
Definition
connects cells for conjugation, produced by donor cell
Term
F plasmid
(fertility plasmid)
Definition
contains genes for DNA replication and several transposable elements to permit integration into the host chromosome (used during conjugation)
Term
tra genes
Definition
genes used for transfer functions between hosts in conjugation
Term
F+
Definition
cells containing an F plasmid that is not integrated into the chromosome
Term
Hfr (high frequency or recombination) cells
Definition
cells with an F plasmid integrated into the chromosome
Term
transposable elements
Definition
stretches of DNA that can move from one site to another
always inserted into another DNA molecule and do not possess their own origin of replication
Term
transposition
Definition
process by which transposable elements move
Term
insertion sequences
Definition
simplest type of transposable element

short DNA segments ~1Kbp long, contains inverted repeats and genes encoding transposase
Term
transposase
Definition
enzyme needed for transposition
Term
transposons
Definition
larger transposable element

has inverted repeats at both ends and a gene for transposes so any gene within the inverted repeats is moved
Term
conservative
Definition
mechanism of transposition where transposon is excised from one location and reinserted at another location so copy number remains the same
Term
replicative
Definition
mechanism of transposition where a new copy of the transposon is made and inserted at the 2nd location so the copy number increases with each transposition
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