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Lecture 26
brock
25
Microbiology
Undergraduate 3
04/08/2014

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Term
mitosis
Definition
process that follows DNA replication in eukaryotes

normal form of nuclear division that results in 2 diploid daughter cells
Term
meiosis
Definition
process of conversion from diploid to haploid state in eukaryotes

specialized form of nuclear division that results in 4 haploid gametes
Term
use protein primer that stays attached to 5' end of DNA instead of RNA primer
Definition
how do viruses and bacteria with linear chromosome replicate 5' end of DNA?
Term
telomerase
Definition
enzyme used by eukaryotes to replicate the ends of the chromosomes by binding to G-rich sequence and becoming active

makes long extension of leading strand so RNA polymerase can attach to the strand
Term
telomeres
Definition
ends of chromosomes that contain repetitive DNA with G-rich 3' end
Term
RNA processing
Definition
alterations done to RNA molecules before they carry out their role in the cell
Term
splicing
Definition
process by which introns are removed and exons joined
Term
spliceosome
Definition
large complex in nucleus that performs RNA splicing
Term
capping
Definition
processing step in the nucleus that adds a methylated guanine nucleotide to the 5' phosphate end of mRNA
(added in reverse orientation)
Term
poly(A) tail
Definition
trimming 3' end of the primary transcript and adding 100-200 adenlate residues as tail (part of RNA processing)
Term
ribozymes
Definition
RNAs with catalytic activity

participate in some cellular reactions, including polypeptide synthesis
Term
self-splicing introns
Definition
introns that fold to generate 3D structures with ribozyme activity

can excise themselves and join adjacent exons
Term
RNA polymerase I
Definition
protein in eukaryotes that transcribes genes for the 2 large rRNA subunits
Term
RNA polymerase II

(similar to archaeal polymerase)
Definition
protein in eukaryotes that transcribes protein-coding genes
Term
RNA polymerase III
Definition
protein in eukaryotes that transcribes genes for tRNAs, 5S rRNA, and other small RNA molecules
Term
TATA box and initiator element
Definition
promoter recognized by RNA Pol II
Term
bacteria: N-formylmethionine

archaea: methionine with ribosome-binding site

eukarya: methionine with no ribosome-binding site (recognizes the cap)
Definition
name the initiator tRNA that recognizes the start codon and inserts the first amino acid for each domain
Term
RNA interference

RNAi
Definition
cleaves dsRNA and destroys ssRNA corresponding to the targeted dsRNA sequence

only found in eukaryotes
Term
Dicer
Definition
DNA specific nuclease which cleaves dsRNA over 20 bp long in eukarya into siRNA
Term
short interfering RNA

siRNA
Definition
dsRNA fragments that are 21-23 bp long because they were cleaved by dicer
Term
RNA-induced silencing complex (RISC)
Definition
complex that recognizes and destroys ssRNA that corresponds to siRNA (fragments of ssRNA bind to this complex)
Term
slicer
Definition
nuclease that is part of RISC and destroys certain ssRNA
Term
microRNA

miRNA
Definition
small dsRNA molecules that regulate translation in eukaryotic cells
Term
Drosha
Definition
enzyme related to dicer that cuts pre-miRNA before it exits the nucleus
Term
miRISC
Definition
protein complex that binds with miRNA and cellular mRNA to block translation without degrading the mRNA
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