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Lecture 23
broc
33
Microbiology
Undergraduate 3
04/08/2014

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Term
transcription
Definition
synthesis of RNA using DNA as a template
Term
mRNA
Definition
carries genetic info. from the genome to the ribosome
Term
rRNA
Definition
has functional and structural role in ribosomes
Term
tRNA
Definition
carries amino acids for protein synthesis
Term
RNA polymerase
Definition
enzyme that carries out transcription

catalyzes the formation of phosphodiester bonds between ribonucleotides using single strand of DNA as template
Term
five subunits: beta, beta', alpha, omega, and sigma

sigma factor dissociates easily from complex
Definition
how many subunits does RNA polymerase have?
Term
promoters
Definition
initiation sites on DNA recognizes by sigma factor of RNA polymerase
Term
transcription bubble
Definition
open double helix once RNA polymerase is bound to double helix
Term
sigma70 or RpoD
Definition
sigma factor that is used for most genes
Term
pribnow box
Definition
part of promoter region located 10 bases before the site of transcription in bacteria
Term
-35 region
Definition
part of promoter region located 35 bases before the site of transcription in bacteria
Term
intrinsic terminators
Definition
sequence patterns that terminate transcription without additional factors eg. stem-loop or secondary structure of RNA
Term
factor-dependent termination
Definition
requires the action of a regulatory protein to terminate transcription
Term
Rho
Definition
regulatory protein that terminates transcription
Term
transcription unit
Definition
segments of DNA transcribed into a single RNA molecule that are bounded by transcription initiation and termination sites

can contain single or multiple genes
Term
polycistronic mRNA
Definition
mRNA encoding a group of cotranscribed genes that when translated creates several polypeptides
Term
operon
Definition
group of related genes transcribed together to yield polycistronic mRNA
Term
catalytic and structural
Definition
two major classes of proteins
Term
proteins
Definition
polymers of amino acids linked by peptide bonds
Term
amino acid
Definition
amino group, carboxylic acid group, H, and side chain attached to carbon
Term
L-amino acids

the L enantiomer
Definition
natural proteins mirror image pair
Term
primary structure
Definition
linear sequence of amino acids in a polypeptide
Term
translation
Definition
synthesis of proteins using the information in mRNA
Term
genetic code
Definition
correspondence between the nucleic acid template and the amino acid sequence of the polypeptide

written in RNA
Term
codon
Definition
triplet bases that correspond to an amino acid
Term
22 amino acids

64 codons
Definition
How many amino acids and codons are there?
Term
AUG
Definition
start codon
Term
UAA, UAG, UGA
Definition
stop codons
Term
anticodon
Definition
complementary sequences of 3 bases found on tRNAs used to recognize codons
Term
wobble
Definition
irregular base pairing at the 3rd position of the codon
Term
Shine-Dalgarno sequence
Definition
regulatory sequence in bacterial mRNA that aligns reading frame for translation

about 8 bases upstream of AUG
Term
open reading frame (ORF)
Definition
start codon followed by codons and a stop codon in a single reading frame

considered putative protein
Term
codon bias
Definition
the multiple codons for a single amino acid are not used equally for a given species

based on tRNAs present
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