Term
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Definition
| Food, structural, markers for protein folding and transport and cell adhesion |
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Term
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Definition
| Membrane, energy storage, signaling |
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Term
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Definition
| Info, catalytic, regulatory |
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Term
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Definition
| Enzymes, antibodies, transporters, structural |
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Term
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Definition
| Nonpolar, polar, and charged (basic or acidic) |
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Term
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Definition
| Alpha helixes, beta sheets, loops, N terminus, C terminus |
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Term
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Definition
| Different tertiary subunits bonded together |
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Term
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Definition
| Beta (top) subunit = 1 extracellular part, 1 transmembrane part. Lambda subunit = transmembrane. Alpha subunit (bottom) = 10 transmembrane parts, 2 intracellular parts. |
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Term
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Definition
| Fluorescent dye/antibody stains cell, look at it w/fluorescing microscope. Cells must be dead, permeabilized, and fixed. |
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Term
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Definition
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Term
| Fluorescence recovery after photobleaching (FRAP) |
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Definition
| Rate of protein movement. Dye a cell fluorescent, bleach part w/laser to kill the proteins there, see how quickly it fills back up w/fluorescent proteins. |
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Term
| Fluorescence resonance energy transfer (FRET) |
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Definition
| To see if proteins interact. Dye 1 protein w/specific fluorescence, dye second protein w/other specific fluorescence. First protein's emission excites the second protein so if the second protein glows they associate. |
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Term
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Definition
| Use a pH separation in addition to a size separation to separate in the Y axis by size and the X axis by charge. Resolves a few thousand proteins, no longer in use. |
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Term
| ID of proteins by mass spectrometry |
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Definition
| Digest protein sample w/protease. Ionize peptides in a mass spectrometer, obtain mass spectrum, use computer to match spectrum w/database values. |
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Term
| Shotgun mass spectrometry |
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Definition
| Digest protein MIXTURE w/trypsin, fractionate peptides w/liquid chromatography and electrospray ionization. Randomly select peptides from first MS, collision-induced fragment (CID) the peptides, and analyze again to get a smaller complex's mass spec. Use computers for database search. |
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Term
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Definition
| Label 2 cultures w/either heavy or light amino acids. Mix proteins. Peptides will form layers based on weight, amount of layer = amount of that protein. |
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Term
| Proteomic analysis of subcellular localization |
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Definition
| Isolate desired organelle w/subcellular fractionation. Use mass spec to determine its protein components. |
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Term
| Proteomic analysis of protein-protein interactions |
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Definition
| Isolate protein complexes w/gentle procedures. Determine protein components by mass spec. |
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