Term
|
Definition
-adaptive immunity (specific)
-B cells are main players
-extracellular spaces bathed in Ig's
-Ig production
-immunity can be transferred to another naive individual |
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Term
|
Definition
-adaptive (specific)
-T cells are main players (CD8 and CD4)
-cannot be transferred to a naive recipient |
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Term
| naive B cells meet antigen |
|
Definition
-meet in secondary lymphoid tissue (lymph node or spleen)
-encounter complementary antigen
-activated by thymus dependent or thymus-independent methods |
|
|
Term
| conventional dendritic cells |
|
Definition
-made in bone marrow (myeloid lineage)
-professional antigen presenting cell
-take up antigen in peripheral tissues by phagocytosis
-present antigen on MHC
-reside in tissues
-travel to peripheral secondary lymphoid organs via lymph vessels |
|
|
Term
| follicular dendritic cells |
|
Definition
-origin unknown
-found in follicles of secondary lymphoid organs
-present antigen to B cells |
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Term
| B cell immune response phase I |
|
Definition
-primary lymphoid follicles
-no germinal center
-B cells proliferate
-prompt secretion of mostly IgM isotype |
|
|
Term
| B cell immune response phase II |
|
Definition
-secondary lymphoid follicles contain germinal centers
-more effective B cell response
-somatic hypermutation
-class switching |
|
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Term
|
Definition
-water in oil
-oil in water
-aluminum hydoxide AlOH |
|
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Term
|
Definition
-coat microtiter plate with purified antigen. let sit for 30-60 minutes and wash away unbound with buffer.
-cover with unrelated protein and wash again
-add serum to be tested for specific antibody and allow to bind. wash off unbound antibody
-add anti-Ig that binds to Fc portion. wash off unbound
-add chromogenic substrate. incubate to develop color
-measure color in spectrophotometer |
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