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Lecture 11
Internal Organelles
22
Microbiology
Undergraduate 2
02/14/2012

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Term
Spores are found almost exclusively in?
Definition
Gram - bacteria. Often in pathogenic bacteria.
Term
Spores are viable for hundreds of years or much longer due to what?
Definition
They are very resistant to heat, irradiation, chemical disinfectants, and desiccation.
Term
What is the difference between terminal, and central endospores?
Definition
Terminal endospores are seen at the poles of cells, whereas central endospores are more or less in the middle.
Term
What is Bacillus sphaericus?
Definition
Spore found, and revived from stomach contents of bee trapped for 25 million years in amber.
Term
What is the structure, and function of an endospore?
Definition
Aid in survival when nutrients or moisture scarce. Have a complex structure of many layers.
Term
15% of the cell dry weight is?
Definition
Dipicolinic acid complexed with calcium which stabilizes the spore nucleic acids.
Term
What are inclusion bodies?
Definition
Granules of organic or inorganic material that are stockpiled by the cell for future use.
Term
What often encloses an inclusion body?
Definition
A single-layered membrane that varies in composition (some made of proteins; others contain lipids)
Term
Name the four organic inclusion bodies.
Definition
Glycogen, polyhydroxybutyrate, cyanophycin granules, and carboxysomes.
Term
What is glycogen?
Definition
A polymer of glucose units.
Term
What is polyhydroxybutyrate?
Definition
Lipid-like polymer, surrounded by proteins. Most common bacterial carbon reserve material.
Term
What are cyanophycin granules?
Definition
Large polypeptides containing about equal quantities of arginine and aspartic acid.
Term
What are in carboxysomes?
Definition
They contain the enzyme ribulose-1,5,-bisphosphate carboxylase (Rubisco), an enzyme used for CO2 fixation.
Term
Name the three inorganic inclusion bodies.
Definition
Polyphosphate granules, sulfur granules, and magnetosomes.
Term
What are polyphosphate granules? What are they used for? What are some other names for them?
Definition
Linear polymers of phosphates that can be used to make ATP. Also called also called volutin granules, or metachromatic granules.
Term
What is a magnetosome?
Definition
Intracellular chains of magnetite (Fe3O4). 40-100nm, membrane enclosed.
Term
What does a magnetosome do?
Definition
Acts as magnet, orientates cell in magnetic field so the bacteria can swim down into the nutrient rich sediment and away from oxygen.
Term
In what would you find gas vesicles?
Definition
Many cyanobacteria, and other aquatic forms.
Term
What are gas vesicles, and what do they do?
Definition
They are aggregates of large numbers of small hollow cylinders that act as a floating device that allows cells to reach optimum depth for light, oxygen and nutrients.
Term
What are gas vesicle walls made out of?
Definition
Protein. Vesicle walls don’t contain lipid.
Term
Describe the permeability of gas vesicle walls.
Definition
Impermeable to water, but permeable to
atmospheric gases.
Term
What does an internal membrane system do? How common are they?
Definition
They are involved in chemiosmotic generation of ATP (e.g., photosynthesizing bacteria using light energy). Few specialized groups of bacteria have extensive internal membrane systems.
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