Term
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Definition
- Protiens of virus were made radioactive.
- virus inffected bacteria
- blended to remove phage
- Centerifuge to seperate virus and bacteria
- DNA in bacteria and Protein coat in solution
- DNA not radioactive and protein coat is
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Term
| Preperation of bacterial DNA |
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Definition
- Culture of bacteria grown and harvested
- cells are removed and broken to give a cell extract
- the dna is purified from the cell extract
- dna is concentrated
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Term
| DNA purification(glass rod and centerfuge) |
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Definition
- DNA is layered with absolute ethanol collected using a glass rod
- or for low concentraction ethonal is used at a ratio of 2.5:1 and the DNA is centerfuged
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Term
| DNA purification (silica) |
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Definition
- cell extract poored into silica column
- DNA binds to silica
- column centerifuged to removed biochemicals
- water is added and centerifuged again to extra DNA
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Term
| DNA purification(ion exchange) |
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Definition
- cell extract is put in column with ion exchange resin
- salt is added
- centerifuge out the biochemicals
- more salt is added
- centerifuge out DNA
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Term
| CTAB method for isolation of plant DNA |
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Definition
- Add CTAB to cell extract
- centerifuge
- pellat is nucleic-acid CTAB complex
- resuspend pellet in IM NaCl
- ethonal precipitation and ribonuclease treatment
- pure DNA
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Term
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Definition
| detergent and similar to an anion-binding reagent, binding negatively charged molecules which preciptates under low salt |
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Term
| Cleared lysate plasmid purifcation |
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Definition
- add lysozyme + EDA and 25% sucrose to bacterial cells
- then add triton 100 X
- then centerfuge for plasmids on top in a clear lysate
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Term
| alkalic lysis method for plasmid purification |
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Definition
- linear and super coiled plasmid mixed
- bring mixture to ph 12-12.5
- then to ph 7
- the centerfuge
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