Term
what is undefined media or complex media? |
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Definition
growth media whose exact composition and amounts of carbon and nitrogen in the ingredients are unknown |
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Term
What are examples of complex media? |
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Definition
peptones: beef and cheese
phytones: plant extracts and yeasts |
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Term
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Definition
site outside organism or where microbe resides and is possible for infection |
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Term
what are some basic categories of colony morphology? |
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Definition
shape, margin, elevation, texture, pigment
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Term
what types of shapes are there |
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Definition
round, irregular, punctiform |
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Term
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Definition
entire (smooth), undulate (wavy), lobate, filamentous, rhizoid |
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Term
What are some types of elevations? |
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Definition
convex, flat, raised, pulvariate, umbonate |
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Term
why do you use agar slants? |
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Definition
-cultivation and maintenence
-displays a variety of growth characteristics |
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Term
why are agar slants better than plates? |
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Definition
-grow high cell densities
-less contamination
-storage capacity
-don't dry as fast |
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Term
why are agar plates better than slants? |
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Definition
-better use for isolation
-produces single colonies |
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Term
-smooth texture with solid edge
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Definition
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Term
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Definition
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Term
solid growth seeming to radiate outward
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Definition
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Term
rough texture with a crusty appearance
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Definition
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Term
suspended chunks of pieces
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Definition
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Term
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Definition
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Term
growth at top around the edge
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Definition
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Term
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Definition
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Term
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Definition
evenly distributed throughout |
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Term
Benefits of broth cultures |
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Definition
-grow high cell densities
-oxygen requirements can be observed |
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Term
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Definition
difficult to grow and needs special conditions |
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Term
What are undefined (complex) media composed of? |
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Definition
-extracts from plant/animal sources
-rich in nutrients |
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Term
The medium fluid thioglycollate can cultivate which microbes? |
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Definition
aerobic, anaerobic, fastidious, nonfastidious---used for oxygen tolerance
-anaerobic and microaerophilic bacteria |
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Term
what are the effects of resazurin? |
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Definition
-pink when oxidized
-colorless when reduced |
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Term
which type would you find growing throughout the tube? |
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Definition
faculative anaerobe
aerotolerant anaerobes |
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Term
Why is it important to use fresh FTG? |
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Definition
because agar used to slow O2 diffusion, so more time will cause greater O2 diffusion which is not beneficial for anaerobes |
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Term
what does the Gas Pak anaerobic jar do? |
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Definition
created anaerobic, microaerophilic, or CO2- enriched conditions |
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Term
What is the Anaerobic Gas Jar Indicator? |
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Definition
methylene blue strip that turns BLUE when oxidized and colorless when reduced |
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Term
what color will the methylene idicator strip turn in anaerobic conditions? |
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Definition
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Term
what is the function of the condenser lens? |
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Definition
concentrates light and makes illumination of specimen more uniform |
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Term
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Definition
the bending of light as it passes through the objective lens |
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Term
What is the 'limit of resolution'? |
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Definition
the measurment of how far apart two points must be to be viewed as being seperate |
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Term
What is numerical aperature? |
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Definition
len's ability to capture light coming from specimen and use it to make an image |
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Term
what does oil immersion do? |
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Definition
increases NA which makes limit of resolution smaller |
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Term
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Definition
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Term
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Definition
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Term
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Definition
the charged portion of a chromogen (colored molecule) |
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Term
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Definition
auxochrome becomes a + charged by picking up H+ or losing OH-
and is able to stain bacteria |
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Term
Preparation for negative stain |
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Definition
1. begin w/ acidic drop on one side of slide
2. add organisms
3. draw back 2nd slide until width of slide and then push to opposite end
4. let air dry |
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Term
how do you know which are acid fast or nonacid fast |
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Definition
acid fast- reddish purple
nonacid fast- blue |
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Term
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Definition
1. drop of congo red on one side
2. add organisms
3.draw back 2nd slide until width of slide and then slide to opposite side
4. air dry
5. flood with Manevals stain
6. rinse and dry |
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Term
Schaeffer-Fulton Procedure |
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Definition
1. heat fixed emulsion
2. cover smeal w/bibulous paper
3. flood with malachite green
4. steam for 5-10 min
5. rinse
6. counterstain with safranin 1 min
7. rinse and dry |
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Term
what do wet mount and hanging drop preps allow you to do? |
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Definition
determine motility, natural cell size, arrangement, and shape |
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Term
benefits of the hanging drop preparation |
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Definition
allows for long-term observation of a living specimen |
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Term
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Definition
1. add petroleum around mouth of depression slide
2. drop water on cover glass
3. add bact
4. place depression slide over cover glass |
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Term
How do you know if there is motility? |
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Definition
Motility: red (reduced) TTC is seen radiating outward from central stab line |
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Term
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Definition
Tetrazolium salt
-used by bacteria as an e- acceptor
-when oxidized its is colorless and soluble
-reduced: red and insoluble |
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Term
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Definition
infection caused by pathogens that do not cause disease in healthy person |
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Term
in the nitrogen cycle column what are the regions from top to bottom |
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Definition
aerobic
microaerophilic
anaerobic
hydrogen sulfide |
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Term
what is the theory for isolation |
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Definition
to identify unknown microbes by obtaining a pur culture |
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Term
what is the purpose of endospore stain |
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Definition
to detect the presence and location of spores within a cell |
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Term
what are acid fast stains used for? |
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Definition
to identify acid fast bacilli
used to identify bacteria in the genus Mycobacterium
only done when an infection is thought to be about |
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Term
purpose of negative stain |
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Definition
identify morphology and cell arrangement in bacteria that can't withstand heat fixing |
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Term
what is a slightly curved rod |
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Definition
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Term
what is it called if division of cells happen in two planes? three planes? |
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Definition
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Term
when is arrangement and morphology easiest to see |
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Definition
when orgs ar grown in a broth or observed from a direct smear |
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Term
The demand for nutrients and production is proportional to ______. And the ability to transport nutrients in and out of cell is proportional to ______. |
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Definition
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Term
does SA or volume increase more rapidly? |
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Definition
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