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Lab Week 1
microscope, aseptic technique, hand washing, eukaryotic microbes
24
Microbiology
Undergraduate 2
01/25/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Prokaryotes include
Definition
Bacteria and Archaea
Term
Eukaryotes include
Definition
Plants, animals, fungi, and protists
Term
What is the typical size of Prokaryotes?
Definition
1-5 micrometers
Term
What is the typical size for eukaryotes?
Definition
10-100 micrometers
Term
Characteristics of Prokaryotes:
Definition
No membrane bound organelles
No microtubules
No introns
No mitotic division
Single circular chromosome
Term
Characteristics of Eukaryotes:
Definition

Have membrane bound organelles

Have microtubules

Whip-like flagellar movement

Linear chromosomes

Have introns

Go through mitotic division

Term
Protozoans
Definition
Unicellular, eukaryotic, heterotrophic microorganisms. Have a life cycle that includes a vegetative throphozoite, and a resting cyst stage.
Term
Algae
Definition
A diverse group of simple, photosynthetic eukaryotic organisms with uncertain relatedness
Term
Green algae
Definition
common in freshwater and are usually unicellular or colonial. EX: Spirogyra and Volvox
Term
What are some other algal groups?
Definition
Red algae, Brown algae, golden-brown algae, yellow-green algae, and diatoms.
Term
How are algal groups differentiated?
Definition
They are differentiated by their photosynthetic pigments, motility, cell wall material, and storage carbohydrate.
Term
Fungi
Definition

Nonmotile eukaryotes

Cell wall made of chitin

Absorptive heterotroph(secrete exoenzymes then absorb nutrients) 

Saprophytes (decompose dead organic matter)

Parisites

Divided into unicellular yeasts, and filamentous molds.

Term
Condenser Lens
Definition
Concentrates the light and makes illumination more uniform
Term
Refraction
Definition
bending of light
Term
Total magnification
Definition
objective X ocular
Term
What is the practical limit to magnification with a light microscope?
Definition
1300X
Term
Resolution
Definition
clarity of an image
Term
Limit of resolution or resolving power
Definition
Actual measurement of how far apart 2 points must be for the microscope to view them as being separate.
About 0.2 micrometers
Term
Formula to calculate limit of resolution
Definition

Wavelength of light used


(NA Condenser +NA Objective)

Term
Numerical aperture
Definition
Measure of the lens's ability to capture light coming from the specimen and use it to make the image
Term
Dark field microscopy
Definition
Special condenser is used so only the light reflected off the specimen enters the objective
Term
Phase contrast microscopy
Definition
uses special optical components to exploit subtle differences in the refractive indices of water and cytoplasmic components to produce contrast
Term
Fluorescence microscopy
Definition
Uses a fluorescent dye that emits fluorescence when illuminated with UV radiation
Term
Asceptic
Definition
Without contamination of the culture, the sterile medium, or the surroundings
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