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Lab Procedures - CCD
Preparation for Lab Procedures Final
379
Veterinary Medicine
Undergraduate 1
11/19/2022

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Term
Define zoonosis
Definition
any disease or parasite that is transmissible from animals to humans
Term
define parasitology
Definition
the study of a parasitic relationship
Term
define symbiosis
Definition
describes the association between at least 2 living organisms
Term
define symbiont
Definition
each living organism in a symbiotic relationship
Term
List 5 types of symbiotic relationship
Definition
-predator/prey
-phoresis
-mutualism
-commensalism
-parasitism
Term
define a predator/prey relationship
Definition
a relationship where a predator benefits when prey is killed and eaten
Term
describe the characteristics of a relationship of phoresis
Definition
The smaller member is mechanically carried by the larger member. Neither member is dependent on the other party and neither member benefits.
Term
define a mutualistic relationship
Definition
A relationship in which both members benefit.
Term
describe the characteristics of a relationship of commensalism
Definition
A relationship in which one benefits and the other does not benefit. The member that does not benefit is not harmed.
Term
describe the characteristics of a relationship of parasitism
Definition
A relationship in which one member (the parasite) lives on or within the other member (the host). The parasite may cause harm to the host and is metabolically dependent on the host for its survival.
Term
define parasite
Definition
an organism that lives on or in a host of another species and benefits by obtaining nutrition from the host, usually at the hosts expense
Term
define host
Definition
an animal that serves as a biological home for parasites and in which the parasite develops, any organism has the ability to become a host
Term
define monoecious
Definition
contain both sets of reproductive organs and can self fertilize
hermaphrodite
Term
What is another name for a Trematode?
Definition
Flukes
Term
What is another name for Cestodes?
Definition
Tapeworms
Term
What phylum do Trematodes and Cestodes belong to?
Definition
Phylum Platyhelminthes
Term
Name two classes of Cestodes
Definition
Class Eucestoda and Class Cotyloda
Term
What is another name for Cotyloda?
Definition
pseudo tapeworms
Term
What is another name for Eucestodes?
Definition
True tapeworms
Term
What are Nematodes commonly referred to?
Definition
Roundworms
Term
describe Cestodes
Definition
-segmented tape-like flat worms
-monoecious
-the head end has suckers and often has hooks
-no alimentary canal
-no body cavity
Term
describe Trematodes
Definition
-flat, leaf-like and unsegmented
-monoecious (except schistosoma)
-head end has suckers with no hooks
-has an incomplete alimentary canal (no anus)
- has no body cavity
Term
describe Nematodes
Definition
-elongated, cylindrical, and unsegmented
-dioecious
-well developed head end with no suckers or hooks
-has a complete alimentary system
-has a body cavity
Term
define dioecious
Definition
having male and female sexes in separate individuals
Term
define endoparasites
Definition
parasites that dwell within the body
Term
define ectoparasites
Definition
parasites that are on the surface of the body
Term
What is the largest phylum of the animal kingdom?
Definition
Phylum Arthropoda
Term
How are the parasites in Kingdom Protista referred to commonly?
Definition
Protozoans
Term
Are Nematodes endoparasites or ectoparasites?
Definition
endoparasites
Term
Are Trematodes endoparasites or ectoparasites?
Definition
endoparasites
Term
Are arthropods endoparasites or ectoparasites?
Definition
ectoparasites
Term
Are Cestodes endoparasites or ectoparasites?
Definition
endoparasites
Term
What phylum do insects, mites, tics, fleas, and spiders belong to?
Definition
Phylum Arthropoda
Term
Name 3 ways a parasite can be ingested
Definition
-ingesting contaminated vomit, feces, or sputum
-ingest an intermediate host
-ingest a paratenic host
Term
define intermediate host
Definition
an organism that supports the immature or non-reproductive forms of a parasite
Term
define paratenic host
Definition
an organism that harbors the sexually immature parasite but is not necessary for the parasites development cycle to progress
Term
define definitive host
Definition
an organism which supports the adult or sexually mature form of a parasite
Term
Name 5 ways a parasite can be transmitted
Definition
-ingestion of parasite or host of parasite
-direct contact with parasite
-transcutaneous
-transmammary
-transplacental
Term
define transplacental
Definition
transmission through the placenta (in utero)
Term
define transmammary
Definition
transmission during nursing
Term
define transcutaneous
Definition
when the infective stage of a parasite enters through the skin of the definitive host
Term
True or False
A parasite can have more than one way to infect a host.
Definition
True
Term
Name 4 types of paraticides
Definition
Anthelminthic, acaricide, insecticides, antiprotozoal
Term
What does an anthelminthic do?
Definition
they kill helminths (Nematodes, Cestodes, and Trematodes)
Term
What do acaricides do?
Definition
They kill mites and tics
Term
What do insecticides do?
Definition
They kill insects
Term
What do antiprotozoals do?
Definition
They kill protozoans
Term
define vermifuge
Definition
anthelmintic that paralyzes parasites
Term
define vermicide
Definition
anthelmintic that kills parasite and causes it to be passed with feces
Term
What is the difference between a qualitative and a quantitative test?
Definition
A qualitative test results in a yes or a no. A quantitative test gives the approximate degree of infection.
Term
True or False: Quantitative tests should be done no matter the results of qualitative tests.
Definition
False
Term
Is gross examination a qualitative or quantitative test?
Definition
Qualitative
Term
True or False: Diagnostic testing is the only reliable way to tell if an animal has an infestation or infection.
Definition
False, medical history, clinical signs, and other lab tests should be used in conjunction with diagnostic testing
Term
What is a gross examination?
Definition
a visual exam
Term
True or False: Age of a fecal sample does not matter when performing diagnostic testing.
Definition
False
Term
What is the maximum age of a fecal sample that has not been refrigerated?
Definition
Less than an hour old
Term
What is the maximum age of a fecal sample that has been refrigerated?
Definition
Less than 24 hours
Term
What is the volume of fecal matter from a small animal (cat or dog) that is needed for fecal testing?
Definition
Approximately the size of an adult thumb
Term
Explain what a pooled sample is.
Definition
A sample collected from a pen of large animals without a specific animal of origin
Term
Explain how a fecal sample should be submitted.
Definition
should be in a sealed glass or plastic container and labeled with client name, date and time of collection, species and animal identification number (pooled samples do not need to contain the animal id number)
Term
What are the rules for handling a fecal sample?
Definition
-wear protective outer clothing
-wear gloves and wash hands with soap and water after handling
-no food, drink, and tobacco should be in area where tests are performed
-clean up immediately after testing
-record results in medical record immediately after testing
Term
What characteristics of feces should be recorded from a gross visual exam?
Definition
-color
-consistency (fecal score)
-presence of blood
-presence of mucus
-age of feces
-visualization of parasites/obvious parasites
Term
Name 5 types of qualitative fecal testing
Definition
Gross Exam
Direct Smear
Simple Fecal Flotation
Fecal Flotation with Centrifugation
Fecal Sedimentation
Term
Describe the process for direct smear
Definition
-place drop of saline/fecal flotation solution on a slide with equal or less than amount of fecal matter
-mix solution and feces together until solution is homologous
-smear the solution over the slide into a thin film (should be thin enough to read print through)
-remove any large pieces of feces
-place coverslip over the smear
-examine the area of the slide under the coverslip with a microscope
-record any protozoan, cysts, eggs, larvae, or gross parasites seen
Term
What are the advantages and disadvantages of fecal concentration procedures?
Definition
Advantages
-larger volume of feces can be used increasing the likelihood of finding parasites
Disadvantages
-requires more time and equipment to diagnose than gross examination and fecal smear
Term
Define specific gravity
Definition
weight of an object compared with the weight of an equal volume of water
Term
Explain how the theory of specific gravity applies to fecal flotation
Definition
Most ova have a specific gravity less than that of the flotation solutions allowing them to float when fecal matter is mixed with the flotation solution
Term
Name 3 examples of flotation solutions
Definition
sodium nitrate, Sheather solution, Zinc sulfate solution, sodium chloride solution, magnesium sulfate solution
Term
Describe the procedure of simple fecal flotation
Definition
-place approximately a tsp of fecal sample in a 90-150mL waxed paper cup.
-Add approx. 30mL of flotation medium
-using a tongue depressor make an emulsion by thoroughly mixing the solution with the feces until a fecal slurry has been made
-bend the side of the waxed paper cup into a spout and cover spout with cheese cloth
-pour the emulsion through the cheesecloth into a straight sided shell vial
-fill shell vial to the top, then slightly overfill so a meniscus forms above the lip
-place glass coverslip gently on top of the fluid and allow it to settle on meniscus
-allow the coverslip to remain undisturbed on top of vial for 10-20 minutes (no longer than 1 hour)
-remove coverslip carefully picking it straight up and immediately place on slide
-examine the area of the slide under the coverslip with a microscope
-record findings
Term
Name the machine that rapidly rotates a group of test tubes in order to separate the contents of the tubes based on density.
Definition
Centrifuge
Term
Describe the process for centrifuge flotation
Definition
-place approximately a tsp of fecal sample in waxed paper cup with enough solution to make a semisolid solution
-pour the emulsion through a cheesecloth into a second cup until the majority of the liquid is separated from the solid material
-pour the liquid into a 15mL centrifuge tube up to the 1/2 - 3/4 mark
-place the tube into the centrifuge
-balance the centrifuge by placing a second tube filled with approximately the same amount of water as fluid in the first tube in the slot across from the first tube (don't forget to mark tubes)
-centrifuge for 3-5 minutes at approximately 1300-1500rpm
-remove tube and fill tube to the top, then slightly overfill so a meniscus forms above the lip
-place glass coverslip gently on top of the fluid and allow it to settle on meniscus
-allow the coverslip to remain undisturbed on top of vial for 10-20 minutes (no longer than 1 hour)
-remove coverslip carefully picking it straight up and immediately place on slide
-examine the area of the slide under the coverslip with a microscope
-record findings
Term
What type of egg would you find on a fecal sedimentation test?
Definition
Fluke eggs or other parasite eggs that have specific gravities that are heavier than most flotation solutions
Term
What type of stains can help stain protozoan cysts for identification?
Definition
Modified D'Antoni's iodine or Lugols iodine
Term
What are fecal antigen tests used for?
Definition
Detecting the presence of immature and adult parasites in an animal
-useful with pre-patent and single sex parasites
Term
What is another name for a fecal antigen test?
Definition
snap test
Term
What are fecal cultures used for?
Definition
differentiating parasites who eggs and cysts cannot be differentiated/identified with microscopic examination of fresh fecal sample
Term
What are the parasites fecal cultures are most useful in differentiation?
Definition
-large and small strongyles in horses
-coccidial protozoans, Eimeria spp. and Cystoisospora spp.
Term
What types of parasites are detected using the cellophane tape preparation?
Definition
ectoparasites and pinworms
Term
Describe the process used in cellophane tape preparation
Definition
-prepare tape wrapped around tongue depressor with adhesive side out
-for pinworms raise animals tail and press tongue depressor firmly against anus
-for ectoparasites firmly press tongue depressor against pruritic skin
-remove tape and apply to slide with small amount of water or mineral oil
-examine under microscope
Term
What is the name of the test that is used to recover the larvae of nematodes from feces, soil, or animal tissue?
Definition
Baermann Technique
Term
What is occult blood?
Definition
blood present in such a small amount that it is not visible to the naked eye
Term
Why is the presence of occult blood important?
Definition
It can indicate damage to intestinal lining from parasites, gastric ulcers, cancer, or other infections agents
Term
What materials can be used in testing for respiratory parasites?
Definition
-fecal matter
-sputum
-tracheal wash
Term
What parasite is often diagnosed using vomitous?
Definition
Roundworms
Term
What is a tracheal wash?
Definition
a process of using a catheter and sterile saline to "wash" the respiratory tract for centrifugation similar to a fecal flotation
Term
Name the procedure that involves removing urine from the bladder with the used of a needle and syringe.
Definition
Cystocentesis
Term
What is a free catch?
Definition
Urine that is collected in a container as it is exiting the animal
Term
What diagnostic procedure is used to examine urine under a microscope?
Definition
Sedimentation
Term
What characteristics of parasitic ova or cysts should be recorded?
Definition
-size (only if microscope has ocular micrometer)
-shape
-shell appearance
-inner appearance
Term
What is the unit of measurement for parasitic ova or cysts?
Definition
microns or micrometers
Term
What shape options can be observed when viewing parasitic ova or cysts?
Definition
Round
Elliptical
Oval
Pear
Triangular
Rectangular
Term
What types of shells can be notated when viewing parasitic ova or cysts under a microscope?
Definition
-thick/thin
-striated/smooth/pitted
-does it have plugs? are they symmetrical? Are they flat?
-Is there an operculum?
Term
Define operculum
Definition
a hatch-like portion of the shell
Term
What might one notate the inner appearance of parasitic ova or cysts looks like?
Definition
-single cell
-morulated
-egg packets
-sporulated or unsporulated
-larvated
Term
what is another word for a morulated?
Definition
cluster
Term
What is the difference between sporulated and unsporulated?
Definition
sporulated ova contain multiple sporozoites where unsporulated contain only 1 sporozoite.
Term
What does the abbreviation NPSATT stand for?
Definition
No parasites seen at this time
Term
What does the abbreviation NSF stand for?
Definition
No significant findings
Term
True or False: Notating the word negative is sufficient when recording results of fecal testing in a medical record.
Definition
False
Term
What factors can cause variance in results of sample examination?
Definition
-sample collected before adult worms producing offspring
-ova producing potential of parasite is low
-heavy infections cause individual females to produce fewer ova
-ratio of males to females or vice versa
-egg output not consistent
-fecal consistency not ideal (diarrhea dilutes number of eggs, dehydration or anorexia increase number of eggs)
-Immune reaction of host (can slow down, speed up, or stop parasite production)
-testing technique was incorrect for specific type of parasite
-technician error
-incorrect amount of sample used
-age of sample
-bad luck
Term
What term means jointed foot?
Definition
Arthropod
Term
What is the body cavity of an arthropod called?
Definition
Hemocoel
Term
Describe the circulatory system of an arthropod
Definition
composed of a heart-like dorsal tube
Term
True or False: Arthropods are dioecious
Definition
True
Term
True or False: Arthropods are larviparous
Definition
False. They are oviparous
Term
What body systems do arthropods have?
Definition
digestive, respiratory, nervous, excretory and reproductive
Term
Why are arthropods important to veterinary medicine?
Definition
-they act as parasites themselves
-they produce venom or toxic substances
-they serve as intermediate or paratenic hosts for helminth and protozoan parasites
-they serve as vectors for bacteria, viruses, spirochetes, rickettsiae, chlamydial agents, and other pathogens
Term
Is Arthropoda a class, a phylum, an order, or a kingdom?
Definition
Phylum
Term
What subphylum is Class Insecta?
Definition
Subphylum Mandibulata
Term
Name 4 Orders in Class Insecta
Definition
Orders Anoplura, Mallophaga, Diptera, and Siphonaptera
Term
How many subphyla are there in Phylum Arthropoda?
Definition
6
Term
Fleas, lice, and 2 winged flies are part of what subphylum and class?
Definition
Subphylum Mandibulata Class Insecta
Term
Mites and ticks fall into what subphylum, class, and order?
Definition
Subphylum Chelicerata, Class Arachnida, Order Aracina
Term
What is the largest phylum in Kingdom Animalia?
Definition
Phylum Arthropoda
Term
What order do fleas fall into?
Definition
Order Siphonaptera
Term
What order do two winged flies fall into?
Definition
Order Diptera
Term
Lice are from what two orders?
Definition
Orders Mallophaga and Anoplura
Term
What class has the largest number of members in Phylum Arthropoda?
Definition
Class Insecta
Term
What 3 body segments do all members of Class Insecta have in common?
Definition
Head, Thorax, Abdomen
Term
How many pairs of legs do all members of Class Insecta have?
Definition
3
Term
What does the head of a member of Class Insecta contain?
Definition
antennae, ventrally directed mouthparts, and maybe eyes
Term
What is located in the abdomen of the members of Class Insecta?
Definition
reproductive organs
Term
What does the thorax of all insects contain?
Definition
3 pairs of legs and maybe wings
Term
define metamorphosis
Definition
a process of transformation in insects from an immature form to an adult form in 2 or more distinct stages
Term
What is the name of incomplete metamorphosis?
Definition
Simple metamorphosis
Term
What are the 3 developmental stages of simple metamorphosis?
Definition
-egg
-nymph
-adult
Term
What is the difference between a nymph and an adult insect?
Definition
A nymph resembles an adult, but is smaller and cannot reproduce.
Term
What term denotes complete metamorphosis?
Definition
Complex metamorphosis
Term
What are the 4 stages of complex metamorphosis?
Definition
-egg
-larva
-pupa
-adult
Term
True or False: In complex metamorphosis, the stages can resemble each other.
Definition
False
Term
Describe what the larval stage of complex metamorphosis looks like.
Definition
worm-like
Term
How is the pupal stage described?
Definition
resting stage
Term
What is the only stage in both simple and complex metamorphosis that is capable of reproduction?
Definition
Adult
Term
How many body segments do mites and ticks have and what are they called?
Definition
2, Capitulum and Idiosoma
Term
What is the capitulum and what basic functions does it have?
Definition
Mouthparts of tick or mite, fusion between the head and thorax, sucking blood or tissue or holding onto the host
Term
What is an idiosoma?
Definition
abdomen of mites or ticks
Term
What is contained in the idiosoma of a member of Order Acarina?
Definition
all organ systems and 4 pairs of legs
Term
How many legs do adult members of Order Acarina have?
Definition
8
Term
True or False: Members of Order Acarina do not undergo true metamorphosis.
Definition
True
Term
What are the developmental stages of members of Order Acarina and why is this not considered true metamorphosis?
Definition
-egg
-larval
-nymphal
-adult
the larval and the nymphal stages resemble the adults except the larval stage only has 3 pairs of legs
Term
What are the two parts contained in the capitulum?
Definition
Chelicerae and palps
Term
What is the difference between Order Mallophaga and Order Anoplura
Definition
Order Mallophaga are chewing or biting lice and Order Anoplura are sucking lice
Term
Give a basic physical description of lice.
Definition
dorsoventrally flattened, wingless insects with clawed appendages
Term
What is the singular of lice?
Definition
louse
Term
True or False: Lice are not species specific and will infest any species of animal.
Definition
False
Term
What type of parasite are lice?
Definition
Permanent parasites, they remain on the host for their entire life cycle
Term
How are lice transmitted most commonly?
Definition
Direct contact between same species
Term
Other than direct contact, what is another way lice can be transmitted?
Definition
contact with contaminated brushes, blankets, bedding etc.
Term
True or False: Only adult lice can be spread by contact via contaminated objects.
Definition
False, eggs, nymphs, and adults can contaminated inanimate objects like bedding or blankets (etc) and spread lice
Term
What is the name for an inanimate object that can be contaminated by eggs, nymphs, or adults of a parasite?
Definition
fomite
Term
What are the hosts of Order Mallophaga?
Definition
mammals and birds
Term
What are the hosts of Order Anoplura?
Definition
Domestic animals (except cats and birds)
Term
What are the characteristics that would make you determine you are looking at a member of Order Mallophaga?
Definition
-3 body segments
-3 pairs of legs
-biting/chewing mouth parts
-Yellow in color with large rounded head
-Head is wider than the widest part of the thorax
-move rapidly over the host
Term
What do the members of Order Mallophaga feed on?
Definition
epithelial debris
Term
What are the characteristics that would make you determine you are looking at a member of Order Anoplura?
Definition
-3 body segments
-3 pairs of legs
-Piercing/sucking mouthparts
-Red/Gray in appearance
-Larger than chewing lice
-Long narrow pointed head (narrower than widest part of thorax)
-Distinct claws on their legs
-move slowly over the host
Term
What do members of Order Anoplura feed on?
Definition
Blood and Tissue
Term
What type of metamorphosis do lice undergo?
Definition
Simple metamorphosis
Term
What is the pre-patent period for lice?
Definition
3-4 weeks
Term
What are lice eggs called?
Definition
nits
Term
Describe the life cycle for lice.
Definition
-glue nits/eggs to hairs or feathers of host
-nits hatch in approx. 5-14 days
-Nymphs developed through 3 nymphal stages to adults in 2-3 weeks
-male and female lice copulate and restart the cycle
Term
What do lice nits look like?
Definition
0.5-1mm white and oval
Term
What is the name for a lice infestation?
Definition
pediculosis
Term
What season is it more common to see a lice infestation and why?
Definition
Winter due to the thicker coat providing a more hospitable environment
Term
What is erythema?
Definition
Skin redness
Term
What is pruritus?
Definition
itchiness
Term
What is a term for hair loss?
Definition
alopecia
Term
What are the clinical signs of a lice infestation (no matter the order of lice)?
Definition
pruritus, erythema, matted fur, roughened haircoat, and alopecia
Term
What are the clinical signs that might suggest an infestation of lice of Order Anoplura or Order Mallophaga?
Definition
small wounds, skin infection, anemia
Term
True or False: A microscope is needed to see even the largest life stage of lice.
Definition
False. All stages of lice are visible with the naked eye.
Term
Describe different ways to diagnose pediculosis.
Definition
-Careful examination of haircoat/feathers reveals lice or nits
-Use of a flea comb or hair sample reveals lice or nits
-Collect individuals with scotch tape or foreceps and examine microscopically
Term
True or False: You do not need to identify the species of lice in order to treat them effectively.
Definition
True. Simply knowing which order they are from will allow you to treat.
Term
Are lice zoonotic?
Definition
No.
Term
What is the maximum amount of time a nymph or adult can live if not on the host?
Definition
7 days
Term
Explain the steps needed to treat for lice?
Definition
-use of most flea and tick products will also kill lice
-treat all in-contact pets of the same species
-wash all bedding in hot soapy water
Term
What other parasite must you treat dogs for after you have cleared the lice infestation?
Definition
Tapeworms/Dipylidium caninum
Term
What is the difference between endoparasites and ectoparasites?
Definition
endoparasites infect and ectoparasites infest
Term
If you have identified pediculosis on a dog and upon microscopic examination find a red-ish, 3 segmented, clawed, 6-legged insect with a pointed head, what Phylym, Subphylum, Class, and Order are they infected/infested with?
Definition
Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Anoplura
Term
If you have identified pediculosis on a cat and upon microscopic examination find a yellow-ish, 3 segmented, 6-legged insect with a wide, rounded head wider than the widest part of the thorax, what Phylym, Subphylum, Class, and Order are they infected/infested with?
Definition
Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Mallophaga
Term
What are the two contrasting roles that flies have as ectoparasites?
Definition
-adults may feed intermittently on vertebrate blood, saliva, and mucus
-larvae may develop in subcutaneous tissues or internal organs of hosts
Term
What type of parasites are flies?
Definition
Periodic parasites
Term
What are the 3 groups flies are classified into?
Definition
-flies in which only the female feeds on blood
-flies that both males and females require blood meals
-flies that feed on mucus, tears, and saliva
Term
True or False: All flies have wings
Definition
False. Melophagus ovinus does not have wings
Term
Describe Melophagus ovinus
Definition
Flattened, wingless, hairy flies that resemble ticks
Term
What is the common name for Melophagus ovinus?
Definition
Sheep keds
Term
What are the hosts for Melophagus ovinus?
Definition
Sheep and goats
Term
True or False: The entire life cycle of Melophagus ovinus takes place on the host.
Definition
True
Term
What are the clinical signs of a Melophagus ovinus infestation?
Definition
Anemia, pruritus, wool damage and loss, dark brown staining on wool
Term
What stains the wool of sheep with a Melophagus ovinus infestation dark brown?
Definition
The feces from the ked
Term
You have been called to examine the sheep of a farmer who has noticed their flock is pruritic and lethargic. Wool production is down and he has noticed dark brown staining on the wool. What is the common name for the parasite they are infested/infected by?
Definition
Sheep keds
Term
You have been called to examine the sheep of a farmer who has noticed their flock is pruritic and lethargic. Wool production is down and he has noticed dark brown staining on the wool. What phylum, subphylum, class, order, genus and species are they infected by?
Definition
Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Diptera, Melophagus ovinus
Term
True or False: Melophagus ovinus or sheep keds are a myiasis producing fly.
Definition
False
Term
What condition is it when dipteran larvae develop in tissues or organs of vertebrate hosts?
Definition
Myiasis
Term
What are the two types of myiasis?
Definition
Facultative myiasis and obligatory myiasis
Term
What is facultative myiasis?
Definition
fly larvae that are normally free living become adapted to being parasitic and dependent on a host to survive to the next stage of the life cycle
Term
What is obligatory myiasis?
Definition
fly larvae are completely parasitic and dependent on the host during development through the life cycle
Term
What are the larvae of flies called?
Definition
maggots
Term
True or False: Facultative myiasis producing flies feed on live tissue.
Definition
False, they only feed on dead cells, exudate, and debris
Term
What is exudate?
Definition
secretions
Term
What is a maggot infestation from facultative myiasis production flies called?
Definition
Fly strike or strike
Term
What are the common names of 4 different types of facultative myiasis producing flies?
Definition
House flies, blowflies, bottle flies, and flesh flies
Term
Explain how a fly strike happens
Definition
A house fly, blowfly, bottle fly or flesh sly is attracted to feed on an animal's moist wound, skin lesion, or soiled hair coat. During feeding they lay their eggs which hatch into maggots. The maggots tunnel through the skin and produce tissue cavities that irritate, injure and kill successive layers of skin.
Term
How can animals die from fly strikes?
Definition
They die from sepsis when the fly strikes become infected.
Term
How do you determine which genus of fly is infesting an animal?
Definition
Examine the spiracular plate on the posterior end of fly larvae and compare it to the dichotomous key
Term
What is a spiracular plate?
Definition
The plate on the posterior end of fly larvae that can be used to identify the genus of fly infesting an animal
Term
What is the name of the key used to help identify which fly is infesting an animal?
Definition
Dichotomous key
Term
What is the genus and species that must be ruled out when determining which type of facultative myiasis producing fly is infesting an animal?
Definition
Cochliomyia hominivorax
Term
What is the common name for Cochliomyia hominivorax?
Definition
Screwworm, primary screwworm, or New World Screwworm
Term
What is the host of the screwworm?
Definition
any warm-blooded species
Term
Is Cochliomyia hominivorax zoonotic?
Definition
Yes and potentially fatal
Term
What is the geographic distribution of Cochliolmyia hominivorax?
Definition
North America and tropical South America and Caribbean Islands (currently eradicated from US)
Term
Why is it so important to identify Cochliomyia hominivorax?
Definition
It is a reportable disease that must be reported to USDA due to the fast moving devastation it causes on livestock and that it feeds on live flesh. Furthermore, it is currently eradicated in the US.
Term
True or False: Cochliomyia hominivorax are a non-biting species that mates often.
Definition
False. While they are a non-biting species, they only lay once in their lifetime.
Term
How many eggs do female screwworms lay on fresh skin wounds?
Definition
15-500
Term
Why is Cochliomyia hominivorax so deadly?
Definition
Third stage larvae are the identifiable larvae and they may take up to 7 days to develop. An animal can die from secondary infection or toxicity in this same amount of time
Term
How long before Cochliomyia hominivorax become third stage larva?
Definition
5-8 days (up to 24 hours to hatch and 4-7 days for becoming 3rd stage larva)
Term
Describe the appearance of 3rd stage larva of Cochliomyia hominivorax.
Definition
They look like a spiraling wood screw. They are segmented, with each segment containing rows of tiny, backward-facing black spines. They possess 2 deeply pigmented tracheal tubes that are parallel on the dorsal-posterior third of the larva.
Term
Describe the life cycle of Cochliomyia hominivorax.
Definition
Male and female mate one time. Female lands on fresh wound and lays eggs at the edge of the wound. Eggs hatch and larvae enter the wound and feed for 4-7 days until they become 3rd stage larva. When fully grown, the larva drop to the ground where they adult fly emerges and the cycle starts again.
Term
What are the clinical signs of a Cochliomyia hominivorax infestation?
Definition
enlarging, draining, foul-smelling wounds, weight loss, death
Term
What is it difficult to see the maggots on the surface of the wound?
Definition
Only the breathing tubes are exposed
Term
How is a Cochliomyia hominivorax infection diagnosed?
Definition
-visualization of larvae with pigmented tracheal trunks, backward-facing rows of spines and distinctive spiracular plates
-samples of eggs, larvae, and flies can also be placed in 70% alcohol and sent to a diagnostic laboratory
Term
How do you treat an animal with a Cochliomyia hominivorax infestation?
Definition
quarantine until daily wound care and treatments with larvicides and insecticides have successfully eliminated infestation
Term
How do you control and eventually eradicate Cochliomyia hominivorax?
Definition
Release sterile males. Because they only mate once in their life, systematically releasing sterile males will eventually eradicate the flies.
Term
What genus is commonly called wolves or warbles?
Definition
Cutebra spp.
Term
What are the primary hosts of Cutebra spp.?
Definition
Rabbits and Rodents
Term
Where are the eggs of Cutebra spp. laid?
Definition
along the entrances of burrows
Term
What is the larvae of Cutebra spp. called?
Definition
Bots
Term
What is the geographic distribution of Cutebra spp.?
Definition
North America
Term
Where on an animal is the most common place to find a Cutebra spp. infestation?
Definition
Face and neck area
Term
How is Cutebra spp. transmitted?
Definition
Through contact with first stage larva
Term
What type of metamorphosis do Cutebra spp. undergo?
Definition
Complex metamorphosis
Term
Describe the life cycle of Cutebra spp. flies.
Definition
Female lays the eggs along the entrances of burrows. They hatch to L1 larva which find their way into the hair of the host. They enter the skin through existing opening of the skin like nares, wounds, and mouth. Larva migrate through the host to the skin and create a breathing whole. They mature in approximately one month. They leave the host and pupate in the ground over winter. Adults emerge in the spring and mate and start the cycle again.
Term
Other than rabbits and rodents, what are the hosts for Cutebra spp.?
Definition
Cats and dogs that wander close to burrows
Term
True or False: Cutebra spp. are known to migrate through any tissue that might get in their way. This includes brain, spinal cord, nose, eye, and pharynx.
Definition
True
Term
Are Cutebra spp. obligatory or facultative myiasis producing flies?
Definition
obligatory
Term
What is a fistula?
Definition
Central pore
Term
How are Cutebra spp. diagnosed?
Definition
Visualization of swollen, cyst-like subcutaneous lesion with a fistula or central pore that communicates with the outside environment.
Term
What is the treatment for Cutebra spp.?
Definition
surgical removal of 2nd or 3rd stage larva
Term
True or False: Intact removal of Cutebra spp. larva is not required.
Definition
False, if the larva is crushed it can produce an anaphylactic response in the host
Term
What are the common names associated with Hypoderma spp. flies?
Definition
Gadflies, warble flies, heel flies (adults), ox warbles, and cattle grubs (larvae)
Term
What is the main host of gadflies?
Definition
Cattle
Term
What is the geographic distribution of Hypoderma spp. flies?
Definition
United States and Canada
Term
How are Hypoderma spp. transmitted?
Definition
Adult female fly deposits eggs on hairs of eggs
Term
What is the typical area of infestation for Hypoderma spp. flies?
Definition
Subcutaneous tissue usually along the dorsum
Term
What type of infestation/infection would be indicated if you have cattle with decreased milk production and and large boil-like cysts along the dorsum
Definition
Hypoderma spp.
Term
How long does the full life cycle for Hypoderma spp. flies take?
Definition
Approximately one year
Term
Explain the life cycle of Hypoderma spp. flies?
Definition
Adult female lays eggs on hairs of cattle legs
Eggs hat into larvae which penetrate into host's body
Larvae migrate until they reach the back
Grubs then cut breathing holes and feed on fleshy skin
Once they reach maturity, they exit through the hole and fall to ground
Grub enters the ground and pupates
Adult fly emerges
Term
What are clinical signs associated with a Hypoderma spp. infestation?
Definition
-cattle running from flies
-large, boil-like cysts with central breathing hole along the dorsum
-hide damage and decreased milk production
Term
What is gadding?
Definition
When cows run from flies
Term
Why must caution be taken when applying insecticides to treat a gadfly infestation?
Definition
If insecticide is used during the migration, you run the risk of the larvae burrowing through the spinal cord and causing death of the cow.
Term
Are Hypoderma spp. flies facultative or obligatory myiasis producing flies?
Definition
Obligatory
Term
What are the common names associated with Gasterophilus spp. flies?
Definition
Bot flies (adults), horse bots, or stomach bots (larvae)
Term
What are the hosts for Gasterophilus spp. flies?
Definition
Horses and donkeys
Term
How are Gasterophilus spp. flies transmitted?
Definition
Adult female fly lays eggs on hairs of the equine leg, equine then grooms itself stimulating the eggs to hatch, the larvae penetrate the mucosa of the lips, tongue and buccal cavity
Term
What is the location of infestation of the larva of Gasterophilus spp. flies?
Definition
Gastric mucosa
Term
What is the taxonomy of Gasterophilus spp.?
Definition
Kingdom Animalia, Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Diptera
Term
What is the taxonomy of Hypoderma spp.?
Definition
Kingdom Animalia, Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Diptera
Term
what is the taxonomy of Cutebra spp.?
Definition
Kingdom Animalia, Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Diptera
Term
Explain the life cycle of Gasterophilus spp.?
Definition
Adult fly deposits eggs on legs chin and shoulders in late summer/early fall
Horses lick area and stimulated larva to hatch
larvae penetrate mucosa of lips, tongue and buccal cavity
larvae migrate through oral mucosa and eventually end up in the stomach where they remain attached for 10-12 months
They then pass out of the host in feces and pupate on the ground for 3-5 weeks when the adult fly emerges
Term
What are the clinical signs of a Gasterophilus spp. infestation?
Definition
Larvae are usually non-pathogenic however adult flies can be annoying to the horse
Term
How would you diagnose a Gasterophilus spp. infestation?
Definition
Visualization of eggs on hairs or 3rd stage larva in feces
Term
What is a bot knife?
Definition
A metal tool used to remove eggs of Gasterophilus spp. flies
Term
How is a Gasterophilus spp. infestation treated?
Definition
Dewormers and the use of a bot knife to remove eggs from hairs
Term
Are Gasterophilus spp. zoonotic?
Definition
No
Term
What are the common names of Oestrus ovis?
Definition
nasal bot fly (adults) and grub (larvae)
Term
What are the hosts of Oestrus ovis?
Definition
Sheep and sometimes goats
Term
How are Oestrus ovis transmitted?
Definition
Adult fly lays eggs in the nares
Term
What is the location of an Oestrus ovis infestation?
Definition
Nasal passages
Term
what are the clinical signs of an Oestrus ovis infestation?
Definition
sneezing, nasal discharge, rubbing nose
Term
How is an Oestrus ovis infestation treated?
Definition
Ivermectin
Term
what is the taxonomy of Oestrus ovis?
Definition
Kingdom Animalia, Phylum Arthropoda, Subphylum Mandibulata, Class Insecta, Order Diptera
Term
Do Hypoderma spp. infect or infest?
Definition
infest
Term
Do Oestrus ovis infect or infest?
Definition
infest
Term
What is the common name of Order Siphonaptera?
Definition
Fleas
Term
What ectoparasite can be described as laterally compressed, wingless insects with legs for jumping?
Definition
Fleas
Term
What class is Order Siphonaptera?
Definition
Class Insecta
Term
What type of mouthparts do Order Siphonaptera have?
Definition
piercing/sucking
Term
What do Order Siphonaptera feed off of?
Definition
Blood
Term
What genus and species of flea are cats and dogs both hosts of?
Definition
Ctenocephalides felis
Term
What climate do the members of Order Siphonaptera prefer?
Definition
Warm humid climates
Term
Name the genus and species for two parasites that Order Siphonaptera
Definition
Dipylidium caninum and Acanthocheilonema reconditum
Term
What type of metamorphosis do fleas go through?
Definition
complex metamorphosis
Term
Name the genus and species of flea that only infests dogs
Definition
Ctenocephalides canis
Term
Describe the life cycle for Order Siphonaptera
Definition
24-48 hours after taking a blood meal and mating on the host, the female lays eggs either in the environment or on the host where they fall off
Egg hatch within 2 weeks
After approximately 1 week, larvae produce a silk-like cocoon in which they become pupae
Depending on the environment, anywhere from 1 week to 1 year later the adult flea will emerge and seek its first blood meal
Term
Describe flea eggs
Definition
pearly white, oval, with rounded ends, approximately 0.5 mm
Term
Describe flea larva
Definition
slender, white, segmented, sparsely covered with short hairs, 1-5 mm long
Term
Of the following ectoparasites, which lay sticky eggs:
Ctenocephalides spp, Order Anoplura, Order Mallophaga, or Dermocentor spp.
Definition
Orders Anoplura and Mallophaga
Term
What is flea dirt?
Definition
flea feces
Term
What do flea larvae feed on?
Definition
organic debris including flea feces
Term
Where would you find flea larvae?
Definition
Away from light, deep in grass or carpet fibers
Term
What stimulates an adult flea to leave its cocoon?
Definition
Air pressure, vibrations, and warmth
Term
How long can an unfed flea survive?
Definition
1 week
Term
How long can a fed flea survive?
Definition
Up to one year
Term
What is a flea infestation called?
Definition
Siphonapterosis
Term
What are the clinical signs of a siphonapterosis?
Definition
pruritus, alopecia, secondary infections, presence of flea dirt, miliary dermatitis, and anemia
Term
Where on the body would you be most likely to see signs of a flea infestation?
Definition
Tailhead/dorsolumbar region
Term
How can you tell the difference between flea dirt and standard dirt?
Definition
Collect and put on a paper towel, wet the paper towel, if it turns red/rust then it is flea dirt
Term
How do you diagnose siphonapteras?
Definition
All life stages can be seen with the naked eye
part hair to find adult fleas or presence of flea dirt
Use a flea comb to find fleas or eggs
Term
What steps should you take to treat and prevent fleas infestations?
Definition
Treat the yard (either with a pest company or a DIY pest controller)
Treat the house (either with a pest company or a DIY pest controller)
Treat all pets in the house
Term
Are Ctenocephalides spp. zoonotic?
Definition
No, humans can be bitten but do not get infestations of fleas, they do have their own species Pulex irritans
Term
What human infectious diseases are fleas vectors for?
Definition
Yersinia pestis (Bubonic plague), Rickettsia typhi and Rickettsia felis
Term
What is a palp or pedipalp?
Definition
leg like structures usually located near the mouth of mites and ticks that act as sensors and assist in attachment to the host
Term
Define caruncle
Definition
sucker at the end of the pedicel of some mite legs
Term
Define chelicerae
Definition
the first appendages near the mouth of mites and ticks used in feeding to pierce or cut the host's skin
Term
Define festoons
Definition
grooves or notches present on some hard ticks that are useful for identification and allow expansion of body during feeding
Term
Define hypostome
Definition
the long appendage a tick uses to insert into its host to collect blood
Term
define mange
Definition
general term used to describe the clinical signs associated with a mite infestation
Term
define molt
Definition
the shedding of the outer exoskeleton of insects and arachnids as they develop to the next stage of development
Term
define pedicel
Definition
a stalk-like structure that is attached to the end of a mite's legs. Used for identifying the various Sarcoptiform mites
Term
Define pediculosis
Definition
term used to describean animal infested with lice
Term
What is the development stage of some insects in which the larval form undergoes complex development to the adult form?
Definition
Pupae
Term
What is the hard dorsal shield of the hard tick called?
Definition
Scutum
Term
What is the process by which ticks wait on vegetation to crawl onto mammals that pass by?
Definition
questing
Term
What is the term used to describe an animal infested with ticks or mites?
Definition
Acariasis
Term
What subphylum and class are mites and ticks?
Definition
Subphylum Cheilicerata Class Arachnid
Term
True or False: Mites are insects that occur as parasitic and free-living forms
Definition
False; mites are arachnids that occur as parasitic and free-living forms
Term
What type of parasites are the majority of mites?
Definition
Obligate, permanent parasites
Term
What makes mites obligate, permanent parasites?
Definition
They spend their entire life cycle on the host and have 4 developmental stages
Term
How many body segments do mites have?
Definition
2
Term
How many pairs of legs do mites have?
Definition
4
Term
What two groups are Sarcoptiform mites broken into?
Definition
Burrowing and non-burrowing
Term
What is the name of the family of mites that burrow?
Definition
Sarcoptidae family
Term
What is the name of the family of mites that do not burrow?
Definition
Psoroptidae family
Term
What characteristics do all Sarcoptiform mites have in common?
Definition
-produce severe dermatologic problems in a variety of animals
-all are tiny
-body shape is round to oval
-have legs with pedicels at tips (can be long/short, straight/jointed, have caruncles/no caruncles)
-Pedicels are used to identify the type of mite
Term
What is the basic life cycle of the Sarcoptidae family of mites?
Definition
-male and females breed on the skin surface
-female penetrates the skin and burrows through the epidermis
-over the next 10-15 days, female lays 40-50 eggs within the tunnel and then dies
-within 3-10 days 6 legged larvae emerge from the eggs and exit the tunnel to wander around the skin surface
-molt to 8-legged nymphs and become adults in 12-17 days
Term
What is the common name for Sarcoptes scabiei?
Definition
scabies or sarcoptes
Term
What is different about the naming system for Sarcoptes scabiei?
Definition
all species specific forms are named Sarcoptes scabiei with the species at the end (ex Sarcoptes scabiei var canis = dog scabies)
Term
True or False: Sarcoptes scabiei is species specific
Definition
True
Term
How is Sarcoptes scabiei transmitted?
Definition
direct contact from host to host
Term
Is Sarcoptes scabiei zoonotic?
Definition
Only the canine and feline variants are
Term
Which species of hosts are Sarcoptes scabiei most commonly infesting?
Definition
Dogs and pigs
Term
What are the clinical signs of Sarcoptes scabiei?
Definition
intense pruritus, small red papules, alopecia, scaling, crusting, and excoriations commonly seen in affected locations
Term
Where are the most common areas that dogs are affected by Sarcoptes scabiei?
Definition
ears, elbows, ventrum, and hocks
Term
Where are the most common areas that pigs are affected by Sarcoptes scabiei?
Definition
trunk and ears
Term
What is the Pedal-Pinnal reflex?
Definition
When you scratch the ears of a dog with scabies and their back leg comes up to scratch their ear
Term
How is Sarcoptes scabiei diagnosed?
Definition
-requires superficial skin scrape to a deep skin scrape (up to 8 affected areas)
-should be viewed under microscope with mineral oil
Term
Describe the appearance of Sarcoptes scabiei
Definition
oval shape, short and stubby legs, long unjointed pedicels with suckers seen on the first 2 pairs of legs
Term
How are Sarcoptes scabiei treated?
Definition
There are numerous oral topical and dip treatments available (some of which need to be repeated)
-all animals in the household of the same species should be treated
Term
What does it mean that Sarcoptes scabiei is a self-limiting infestation in humans?
Definition
It will resolve without treatment
Term
What is the more often feline mite, Sarcoptes scabiei var felis or Notoedres cati?
Definition
Notoedres cati
Term
What is the common name of Notoedres cati
Definition
Feline scabies mite
Term
Is Notoedres cati zoonotic?
Definition
Yes
Term
What are the clinical signs of Notoedres cati?
Definition
alopecia, pruritus, and thick crusts on ears face back of neck and feet
Term
How is Notoedres cati diagnosed?
Definition
superficial skin scrapings from several areas and are then viewed under a microscope
Term
What are the identifying characteristics of Notoedres cati?
Definition
oval shape, short and stubby legs, long unjointed pedicels with suckers seen on the first 2 pairs of legs and a subterminal anus
Term
How is Notoedres cati treated?
Definition
-topically REvolution or Advantage multi
-Lime Sulfur Dip
or
-Oral Ivermectin
all animals of same species in the household should be treated
Term
What is the name of the mite that affects birds?
Definition
Cenemidocoptes spp.
Term
What animal is host to Cnemidocoptes pilae?
Definition
budgerigars and parakeets
Term
What animal is host to Cnemidocoptes mutans?
Definition
poultry
Term
What is the common name of Cnemidocoptes spp. ?
Definition
Scaly leg or scaly face mite
Term
Is Cneidocoptes spp a Sarcoptiform or non-sarcoptiform mite?
Definition
Sarcoptiform
Term
Is Cnemidocoptes spp. zoonotic?
Definition
no
Term
What are the clinical signs of Cnemidocoptes spp.?
Definition
yellow/gray white mass on affected areas resembling a honeycomb
causes extreme pruritus on feet, beak, and cere
Term
What is the name of the condition that can be a product of Cnemidocoptes spp. in canaries?
Definition
Tassel-foot
Term
Describe the physical appearance of Cnemidocoptes spp. mite?
Definition
round to oval, females have short legs with no suckers, males have longer legs with long unjointed pedicel and suckers
Term
What is the treatment for Cnemidocoptes spp?
Definition
Isolate and treat all birds with lesions and any birds that have had direct contact with affected bird
-give ivermectin orally in repeated doses
Term
Explain the life cycle for mites in family Psoroptidae?
Definition
5 stage life cycle
-egg
-larva (6 legs)
-2 nymphal stages
-adult
Adults breed on skin surface where female produces 14-24 eggs that hatch larvae within 1-3 days, they molt through their nymphal stages within 7-10 days
Term
Name the two nymphal stages for mites in family Psoroptidae
Definition
Protonymph and Deutonymph
Term
How long does the entire life cycle of a mite in the Psoroptidae family last?
Definition
10-18 days
Term
What is the most glaring physical difference between a mite in the Sarcoptidae family and a mite in the Psoroptidae family?
Definition
legs of a Psoroptidae family mite are quite long where as a mite in the Sarcoptidae family are short and stubby
Term
Are Psoroptes spp mites species specific?
Definition
yes
Term
What is the common name of Psoroptes spp. mites?
Definition
Psoroptic mange, scabies mite, or scab mite
Term
What is the host of Psoroptes ovis?
Definition
Sheep
Term
What is the host of Psoroptes bovis?
Definition
cattle
Term
What is the host of Psoroptes equi?
Definition
horse
Term
True or False: A Psoroptic spp. infestation is reportable to state and federal agencies
Definition
True
Term
Are Psorotes spp. mites zoonotic?
Definition
no
Term
What are the clinical signs of a Psoroptes spp. mite?
Definition
intense pruritus causing the host to rub affected areas against fences, posts, equipment, etc
leads to alopecia and severe excoriations
crusts, papules, wrinkled and thickened skin
Term
What do Psoroptes spp. mites look like?
Definition
Oval with long legs, long jointed pedicels with small caruncles on the tips
Term
How are Psoroptes spp. mites treated?
Definition
Ivermectin or lime-sulfur dips
Term
What mite affects horse, cattle, goats, and sheep mainly on the lower part of the hind legs?
Definition
Chorioptes spp.
Term
Are Chorioptes spp. mites species specific?
Definition
yes
Term
What is the common name for Chorioptes spp. mites?
Definition
Foot and tail mite or itchy leg mite
Term
What part of cattle do Chorioptes spp. mites prefer?
Definition
tail region
Term
What are the clinical signs of a Chorioptes spp. infestation?
Definition
pruritus, crusts, alopecia and scaling in affected areas
skin may eventually wrinkle and become thickened
Term
What phylum, subphylum, class and order of Chorioptes spp.?
Definition
Phylum Arthropoda, Subphylum Chelicerata, Class Arachnida, Order Aracina
Term
What is the physical appearance of a Chorioptes spp. mite?
Definition
Oval with long legs, long jointed pedicels with large caruncles on the tips
Term
What is the common name of Otodectes cynotis?
Definition
ear mites
Term
What is/are the host(s) of Otodectes cynotis?
Definition
dogs cats and ferrets
Term
What is the site of infestation for an Otodectes cynotis?
Definition
Ear canal but may also be found on the tailhead region
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