Term
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Definition
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Term
the mordant in the gram stain |
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Definition
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Term
role of alcohol in gram stain |
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Definition
dissolves lipids in outer membrane and removes the dye from the peptidoglycan layer in GRAM NEGATIVE only |
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Term
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Definition
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Term
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Definition
has thick peptidoglycan wall |
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Term
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Definition
has thin peptidoglycan wall |
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Term
gram positive cells stain |
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Definition
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Term
gram negative cells stain |
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Definition
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Term
examples of gram positive bacteria |
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Definition
Staphylococcus aureus, streptococcus, bacillus |
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Term
examples of gram negative bacteria |
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Definition
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Term
example of endospore forming genus |
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Definition
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Term
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Definition
a survival structure formed within the bacteria wall that allows the cell to survive until the environmental conditions improve |
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Term
red cells in endospore stain |
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Definition
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Term
green cells in endospore stain |
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Definition
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Term
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Definition
waxy cell wall of mycolic acid |
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Term
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Definition
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Term
color of non acid fast cells |
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Definition
blue--stained with methlyene blue |
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Term
type of acid fast bacteria |
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Definition
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Term
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Definition
Mycobacterium, Nocardia TB and leprosy |
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Term
characteristics of good streak plate |
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Definition
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Term
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Definition
bacteria that can grow in a higher salt concentration than 3% NaCl |
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Term
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Definition
organism requiring a temperature of -5C to 15C. only grow below 20 Celsius. |
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
grow in environments below pH 5.5 |
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Term
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Definition
live between pH 5.5 & pH 8.5 |
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Term
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Definition
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Term
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Definition
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Term
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Definition
grow over wide range of salinty |
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Term
Obligate (strict) aerobes |
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Definition
must have oxygen to carry out metabolism |
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Term
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Definition
prefer oxygen in concentrations of 2-10% need less oxygen to grow |
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Term
facultative aerobes (anaerobes) |
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Definition
bacteria that grow well aerobically but can also grow anaerobically if oxygen is not present |
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Term
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Definition
Can tolerate oxygen and can grow but do not require it for energy production. also obligate fermenters because they get their energy strictly by fermentation |
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Term
Obligate (strict) anaerobes |
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Definition
cannot tolerate oxygen and are killed by its presence |
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Term
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Definition
An enzyme that degrades hydrogen peroxide into Oxygen and water. |
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Term
components of fluid thioglycollate medium |
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Definition
A rich liquid medium that supports the growth of both aerobic and anaerobic bacteria. Contains glucose, cystine, and sodium thioglycollate. |
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Term
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Definition
reszurin (indicator of the presence of oxygen) turns PINK in the presence of oxygen |
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Term
Aerobic growth pattern in FTM |
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Definition
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Term
Microaerophillic growth in FTM |
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Definition
not at the top or bottom--1/3 from the top |
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Term
facultative growth in FTM |
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Definition
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Term
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Definition
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Term
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Definition
chemicals that kill microbes on NON-Living Surfaces |
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Term
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Definition
chemicals that are safe on Living surfaces |
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Term
what type of lab is the disinfectant/antiseptic lab? |
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Definition
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Term
what type of lab is the thermal death time lab? |
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Definition
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Term
what is Thermal death time? (TDT) |
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Definition
the length of time at a given temperature that will kill all the cells in a culture |
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Term
color of MSA (mannitol salt agar plate) |
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Definition
bright pink (slightly opaque) |
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Term
selective property of MSA plate |
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Definition
contains a high salt concentration (7.5% NaCl) |
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Term
differential property of MSA plate |
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Definition
contains the sugar mannitol and pH indicator phenol red |
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Term
color of positive fermentation of MSA plate |
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Definition
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Term
MSA plate is selective for what species? |
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Definition
micrococcus and staphylococcus |
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Term
selective properties of Eosin methylene Blue plate (EMB) |
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Definition
eosin and methylene blue inhibit growth of gram positive bacteria (only gram negative can grow) |
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Term
differential properties of EMB plate |
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Definition
contains the sugar lactose which allows differentiation between non lactose fermenters, lactose fermenters, and heavy lactose fermenters |
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Term
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Definition
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Term
color of lactose fermenting bacteria on EMB plate |
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Definition
pink with purple in the center |
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Term
strong or rapid lactose fermenters color on EMB plate |
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Definition
dark purple with metallic green color |
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Term
color of non lactose fermenters on EMB plate |
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Definition
colorless (gram negative bacteria only) |
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Term
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Definition
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Term
what type of growth occurs on Hektoen Enteric Agar? |
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Definition
Selective medium used to isolate gram negative enteric bacteria |
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Term
selective aspect of Hektoen Enteric agar |
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Definition
bile salts (inhibit the growth of gram positive organisms) |
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Term
differential aspect of Hektoen enteric agar |
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Definition
lactose, sucrose, and salicin are added to differentiate between non fermenting enteric bacteria and fermentive enteric bacteria |
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Term
dyes (indicators) added to Hektoen enteric agar |
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Definition
fuschin and bromthymol blue |
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Term
color of Hektoen agar plate with positive fermentation |
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Definition
yellow to pink color Ex: E. Coli |
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Term
color of organisms who do NOT ferment any sugars in Hektoen agar plate |
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Definition
blue/green colonies Ex: Salmonella and Shigella |
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Term
what is added to Hektoen agar to detect for hydrogen sulfide? |
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Definition
Ferric ammonium citrate and sodium thiosulfate--seen as black precipitate Ex: salmonella |
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Term
indicator in Phenol red broth |
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Definition
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Term
tube that collects gases during fermentation |
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Definition
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Term
color of medium when fermentation is positive in phenol red broth |
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Definition
YELLOW (acid by-products are present) |
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Term
color of medium if organism CANNOT ferment the sugar is phenol red broth |
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Definition
magenta/fuschia/BRIGHT PINK b/c protein breakdown increases the pH of the medium this is an alkaline reaction |
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Term
what type of medium is citrate agar? |
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Definition
utilization medium: a defined media in which one or more essential nutrients is limited. |
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Term
pH indicator of citrate test |
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Definition
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Term
color of citrate tube when test is positive |
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Definition
if the bacteria can use citrate as its sole source of carbon the tube will change to from green to blue color is green at 6.9 pH and blue at 7.6 pH. |
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Term
presence of catalase is... |
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Definition
indirect evidence of the ETC |
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Term
color of M. Luteus growth on Mueller hinton agar |
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Definition
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Term
color of P. aeruginosa on MH agar |
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Definition
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Term
color of S. marcescens on MH agar |
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Definition
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Term
what is the purpose of the Phenylalanine Deaminase test? |
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Definition
determines whether or not an organism possesses the enzyme phenylalanine deminase |
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Term
what is the color indicator of the Phenylalanine Deaminase test? |
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Definition
ferric chloride turns deep green if deamination has taken place |
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Term
enzyme that can break down starch |
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Definition
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Term
results of a bacteria that produces amylase |
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Definition
halo around the colony where starch has been "eaten", starch plate will be blue/black |
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Term
reagent in starch hydrolysis test |
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Definition
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Term
enzyme that breaks down peptide bonds between amino acids |
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Definition
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Term
positive result of gelatinase test |
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Definition
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Term
enzyme that can breakdown urea |
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Definition
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Term
pH indicator of urea broth |
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Definition
phenol red (medium will be yellow orange before inoculation) |
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Term
color of urea broth if test is positive |
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Definition
ammonia is released and causes the pH of medium to increase. media will change from yellow/red to bright pink Ex. Proteus |
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Term
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Definition
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Term
the enzyme that is responsible for transferring electrons to oxygen and reducing them to water. |
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Definition
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Term
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Definition
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Term
differential properties of DNase agar |
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Definition
only lets organisms that can enzymatically hydrolyze DNA grow |
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Term
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Definition
plate has clearing (DNA has been eaten up) |
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Term
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Definition
plate is sky blue (DNA has not been eaten) |
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Term
what type of media is SIM media? |
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Definition
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Term
three things SIM media looks for |
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Definition
reduction of sulfur, production of indole (due to breakdown of tryptophan), and motility |
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Term
result of sulfur reducing bacteria in SIM media |
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Definition
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Term
result of indole production in SIM media |
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Definition
positive red color from Kovac's reagent |
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Term
what type of media is the blood agar plate? |
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Definition
enriched and differential |
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Term
differential properties of blood agar |
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Definition
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Term
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Definition
No breakdown of blood agar |
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Term
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Definition
partial clearing/breakdown of blood agar |
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Term
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Definition
complete clearing/breakdown of blood agar |
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Term
the enzyme that can coagulate the blood |
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Definition
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