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Lab Practical 1
N/A
87
Biology
Undergraduate 1
09/24/2012

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Cards

Term
Microscopy
Definition
allows you to see a complex world or microscopic beings usually unseen to the naked eye
Term
Magnification
Definition
makes image appear larger
Term
Resolution
Definition
Fine details, ability to distinguish two objects that are close together as being two seperate objects.
Term
Contrast
Definition
objects absorbtion and transmition of light
Term
Staining
Definition
Technique used to enhance the contrast
Term
stereomicroscop view range
Definition
few hundreths of a mm to a cm
Term
interpulpillary distance
Definition
distance between your eyes
Term
light switches
Definition

left switch is upper light

right switch is lower light

Term
upper light
Definition
opaque (non transparent) objects
Term
lower light
Definition
transparent objects
Term
Focusing knob
Definition
Sharpen the image
Term
Magnification knob
Definition
increases or decreases the object in view
Term
diopter
Definition
on the left eyepiece of the nikon allows you to precisely adjust the sharpness of an image
Term
nosepiece
Definition

four objective lenses

 4x,10x,40x,400x are the objectives

Term
total magnification
Definition

product of the eyepiece and the objective lens magnification

eye piece: 10x

Term
caliper
Definition
holds slide in place
Term
mechical stage
Definition
holds caliper
Term
stage controls
Definition

lower knob moves slide left and right

upper knob moves slide towards or away from you

Term
focusing knobs
Definition
2 knobs used to sharpen images
Term
coarse focus knob
Definition
moves stage up or down quite a distance to permit focusing used with 4x and 10x
Term
fine focus knob
Definition
moves stage vertically only a small distance
Term
condensor
Definition
contains and iris diaphram that can be opened to permit more light to illuminate the slide
Term
bacillus
Definition
.01mm
Term
cheek/amoeba
Definition
.1mm
Term
chick embryo
Definition
4mm
Term
Lecia
Definition

stereomicroscope

magnify 30x

for larger items such as insects

use for more details( can usually see them with naked eye)

3D

Term
Nikon
Definition

compound microscope

.1mm

Magnify 1000x

smaller objects

not visible to naked eye

thin object that light can pass through

not 3D

Term
Lecia Parts
Definition

magnification control knob

eyepiece

light switches

base

focusing knob

Term
Nikon parts
Definition

eyepiece with diopter focusing ring

caliper

coarse focus (outer ring)

fine focus (inner ring)

power switch

eyepiece with pointer

nosepiece

objective

mechanical stage

stage controls

condenser with diaphram

light source

Term
Electrophoresis
Definition
one of the major ways to seperate and identify proteins
Term
Electrophoretic seperation
Definition
depends in great part on the net electrical charge of proteins
Term
If a protein has no net charge...
Definition
it will not move towards negative or positive
Term
carboxyl group
Definition
negative charge
Term
amino group
Definition
positive charge
Term
5 amino acids with ionic R groups
Definition

glutamic acid

aspartic acid

lysine

arginie

histidine

Term
isoelectric point
Definition
pH in between, a particular protein will have no net charge
Term

independent variable

 

Definition

property you change or manipulate in a systamatic way

 

ph of the buffer (4,6,8)

Term
dependent variable
Definition

outcome affected by the independent variable

electrical charge of protein

Term
Graph of Ph and net charge of the protein
Definition

inverse relationship

pH up charge down

pH down charge up

no charge= isoelectric point

downward slope to the right

Term
property that influences protein migration
Definition
charge on the R group and net eletronic charge of the protein
Term
At a lower pH...
Definition

H+ion concentration increases

H+ ions stick to proteins to make it positivly charged

Term
permeable
Definition
a molecule can pass through a cell's membrane
Term
impermeable
Definition
can not pass through a cell membrane
Term
red blood cell plasma membrane
Definition
realtivly simple because it is composed largely of phosphlipids with very few proteins
Term
diffusion
Definition
membrane permeability, water potential and the fragility of animal
Term
independent variable
Definition
size of teh solute molecule the net charge of the solute particle and the lipid solublitly of the solute molecule of teh solute molecule
Term
dependent variable
Definition
cell membrane permeability
Term
spec 20 for membrane permeability
Definition

1. no blank in the holder turn left knob to 0 transmittance which is 100% absorbance.

2. put the blank in turn the right knob to 100% transmittance 0 absorbtion

Term
tranmittance
Definition
right knob amount of light that passes through
Term
absorbtion
Definition
left knob amount of light absorbed lower reading on the spec 20
Term
charged ions
Definition

not permeable

no lyse

sodium ion

chloride ion

Term
size
Definition

as increases less permable

glycerol

urea

Term
lipid solubility
Definition

as increases becomes more permeable

so its a direct relationship

more CH bonds or nonpolar bonds more soluable

trumps size

Term
how to find H50
Definition

time to lyse (bigger)- starting amount(smaller)

+starting amount

Term
H50 increases...
Definition
permability decreases
Term
solute concentration increaeses
Definition
water potential decreases
Term
lyse
Definition
when the cell bursts and the solution becomes clear
Term

as lipid solubility increases

 

Definition
permeability increases
Term
prokaryotes
Definition
bacteria and archea
Term
archea
Definition

similar to eukaryotes

but they are prokaryotes

live in very strange and hostile environments such as salty hot or acidic

Term
bacteria
Definition

most familiar prokaryote

distinguished by 3 shapes

cocci-spherical

rod-shaped-bacilli

helical-spirilla

have cell walls (peptidoglycan)

heterotrophic

or

autotrophic

-aerobic

or

 anerobic

Term
heterotrophic
Definition
feed on other things
Term
autotrophic
Definition
self feeders
Term
aerobic
Definition
needs oxygen
Term
anerobic
Definition
does not require oxygen
Term
protists
Definition

diplomonads

rhizarians

ameoboszoans

euglenids

kinetoplastids

alveolata

stramenopila

rhodophytes

slime molds

Term
diplomads
Definition

move by flagella

looks like a face

lack mitochondria

giardia

heterotrophic

Term
rhizaria
Definition

move by pseudopdia

stiff and project out to catch food

amoeba proteus

heterotrophic

Term
amoebozoans
Definition

move by pseudopodia

amoeba proteus

heterotrophic

Term
euglenids
Definition

move by way of flagella

autotrophic

euglena and trypanosoma

Term
kinetoplastids
Definition

move by flagella

autrophic

one large mitochondria

Term
ciliates
Definition

unicellular

move by cilia

paramecium plasmodium and peridinium

heterotrophic

Term
Apicomplexa
Definition

disease causing internal parasites

unicelluar

heterotrophic

move by cilia

 

Term
Dinoflagellates
Definition

unicelluar

autotrophic

uses chlorophyll

have flagella

perididinium

 

Term
diatoms
Definition

2 flagella

unicellular

autotrophic with cholorphyll

 

Term
brown algea
Definition

autotrophic

move by flagella

multicellular

sargassum and fucus

 

Term
rhod
Definition
Term
rhodophytes
Definition

autotrophs

chlorophyl a and d

batrachospermum

Term
plasmodium
Definition

heterotrophic

a cellular

physarum

take in food by phagocytosis

 

Term
fungi
Definition

zygomycota

basidiomycota

ascomycota

Term
zyomycota
Definition

reproduce sexually by having the hyphae of adjoining individuals extending branches taht make contact, coalesce,and form gametes that fuse togethre to form a zygote that then can develop into a new individual

heterotrophic

Term
basidia
Definition

heterotrophic

reproduce sexally via basidia

Term
ascomycota
Definition

heterotrophic

yeast

reproduce sexually

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