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allows you to see a complex world or microscopic beings usually unseen to the naked eye |
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makes image appear larger |
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Fine details, ability to distinguish two objects that are close together as being two seperate objects. |
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objects absorbtion and transmition of light |
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Technique used to enhance the contrast |
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stereomicroscop view range |
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few hundreths of a mm to a cm |
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distance between your eyes |
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left switch is upper light
right switch is lower light |
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opaque (non transparent) objects |
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increases or decreases the object in view |
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on the left eyepiece of the nikon allows you to precisely adjust the sharpness of an image |
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four objective lenses
4x,10x,40x,400x are the objectives |
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product of the eyepiece and the objective lens magnification
eye piece: 10x |
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lower knob moves slide left and right
upper knob moves slide towards or away from you |
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2 knobs used to sharpen images |
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moves stage up or down quite a distance to permit focusing used with 4x and 10x |
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moves stage vertically only a small distance |
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contains and iris diaphram that can be opened to permit more light to illuminate the slide |
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stereomicroscope
magnify 30x
for larger items such as insects
use for more details( can usually see them with naked eye)
3D |
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Term
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Definition
compound microscope
.1mm
Magnify 1000x
smaller objects
not visible to naked eye
thin object that light can pass through
not 3D |
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Term
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Definition
magnification control knob
eyepiece
light switches
base
focusing knob |
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Term
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Definition
eyepiece with diopter focusing ring
caliper
coarse focus (outer ring)
fine focus (inner ring)
power switch
eyepiece with pointer
nosepiece
objective
mechanical stage
stage controls
condenser with diaphram
light source |
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Definition
one of the major ways to seperate and identify proteins |
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Electrophoretic seperation |
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Definition
depends in great part on the net electrical charge of proteins |
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If a protein has no net charge... |
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Definition
it will not move towards negative or positive |
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5 amino acids with ionic R groups |
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Definition
glutamic acid
aspartic acid
lysine
arginie
histidine |
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Definition
pH in between, a particular protein will have no net charge |
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Definition
property you change or manipulate in a systamatic way
ph of the buffer (4,6,8) |
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Definition
outcome affected by the independent variable
electrical charge of protein |
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Graph of Ph and net charge of the protein |
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Definition
inverse relationship
pH up charge down
pH down charge up
no charge= isoelectric point
downward slope to the right |
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property that influences protein migration |
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Definition
charge on the R group and net eletronic charge of the protein |
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Definition
H+ion concentration increases
H+ ions stick to proteins to make it positivly charged |
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Definition
a molecule can pass through a cell's membrane |
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can not pass through a cell membrane |
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red blood cell plasma membrane |
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Definition
realtivly simple because it is composed largely of phosphlipids with very few proteins |
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Term
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Definition
membrane permeability, water potential and the fragility of animal |
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Definition
size of teh solute molecule the net charge of the solute particle and the lipid solublitly of the solute molecule of teh solute molecule |
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Definition
cell membrane permeability |
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Term
spec 20 for membrane permeability |
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Definition
1. no blank in the holder turn left knob to 0 transmittance which is 100% absorbance.
2. put the blank in turn the right knob to 100% transmittance 0 absorbtion |
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Definition
right knob amount of light that passes through |
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left knob amount of light absorbed lower reading on the spec 20 |
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not permeable
no lyse
sodium ion
chloride ion |
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Definition
as increases less permable
glycerol
urea |
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Definition
as increases becomes more permeable
so its a direct relationship
more CH bonds or nonpolar bonds more soluable
trumps size |
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Definition
time to lyse (bigger)- starting amount(smaller)
+starting amount |
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solute concentration increaeses |
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Definition
water potential decreases |
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when the cell bursts and the solution becomes clear |
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as lipid solubility increases
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similar to eukaryotes
but they are prokaryotes
live in very strange and hostile environments such as salty hot or acidic |
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Definition
most familiar prokaryote
distinguished by 3 shapes
cocci-spherical
rod-shaped-bacilli
helical-spirilla
have cell walls (peptidoglycan)
heterotrophic
or
autotrophic
-aerobic
or
anerobic |
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diplomonads
rhizarians
ameoboszoans
euglenids
kinetoplastids
alveolata
stramenopila
rhodophytes
slime molds |
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Term
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Definition
move by flagella
looks like a face
lack mitochondria
giardia
heterotrophic |
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Term
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Definition
move by pseudopdia
stiff and project out to catch food
amoeba proteus
heterotrophic |
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Term
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Definition
move by pseudopodia
amoeba proteus
heterotrophic |
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Definition
move by way of flagella
autotrophic
euglena and trypanosoma |
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Term
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Definition
move by flagella
autrophic
one large mitochondria |
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Definition
unicellular
move by cilia
paramecium plasmodium and peridinium
heterotrophic |
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Term
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Definition
disease causing internal parasites
unicelluar
heterotrophic
move by cilia
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Definition
unicelluar
autotrophic
uses chlorophyll
have flagella
perididinium
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Term
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Definition
2 flagella
unicellular
autotrophic with cholorphyll
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autotrophic
move by flagella
multicellular
sargassum and fucus
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autotrophs
chlorophyl a and d
batrachospermum |
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Definition
heterotrophic
a cellular
physarum
take in food by phagocytosis
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Definition
zygomycota
basidiomycota
ascomycota |
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Term
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Definition
reproduce sexually by having the hyphae of adjoining individuals extending branches taht make contact, coalesce,and form gametes that fuse togethre to form a zygote that then can develop into a new individual
heterotrophic |
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heterotrophic
reproduce sexally via basidia |
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Definition
heterotrophic
yeast
reproduce sexually |
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