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Lab Module 1 Midterm
Lab 1
20
Microbiology
Graduate
03/09/2011

Additional Microbiology Flashcards

 


 

Cards

Term
Arm
Definition

 

supports the body tube and is the part to grasp when carrying the

microscope.

 

Term
Body tube
Definition

connects the ocular lens with the nosepiece housing the objective

lenses.

Term
Nosepiece
Definition

rotating part to which the three objective lenses are attached.

Term
Objective lenses
Definition

lenses that magnify the images by the power shown on the side of the

objective lens: low-power (4X, 10X), high-dry (40X), and oil-immersion (100X).

Term

Ocular lens

Definition

lens that magnifies the real image another ten times.

Term

 

Stage

 

Definition

 

supports the microscope slide, which is held on to the _____ by a slide

holder.  

-Contains an opening that allows incident light shine through to

the specimen.

 

Term
Condenser
Definition

a lens located directly below the microscope stage that concentrates

the light before it passes through the specimen. There is a knob that can raise or

lower the condenser.

Term

Iris diaphragm (Condenser Aperture)

Definition

an opening on the bottom of the

condenser that regulates the amount of light entering the condenser. A lever

moves back and forth to regulate the size of the opening.

Term

Coarse adjustment

Definition

a rotating knob that moves the body tube up or down to bring the specimen into initial focus.

This knob is used only with the 4X and 10X

objectives. With the high-dry (40X) and oil immersion (100X) objectives, there is

a danger of driving the objectives through the slide.

Term

Fine adjustment

Definition

a smaller rotating knob that brings the specimen

into final focus by moving the body

tube more slowly. As the objective lenses are

parfocal, only the fine adjustment knob needs

to be used after you have focused with the

low-power objective.

Term

Parfocal - lenses

Definition

are arranged such that after focusing with

one objective; the image will remain in focus when moving to another objective

Term
Bright-field Microscope
Definition
Light goes in through the condenser to the specimen; used to visualize stained specimens
Term
Dark-field Microscope
Definition

Light passes through a condenser modified with a dark field stop; light goes around a specimen

 

 

Term
Dark-field Microscope Use
Definition
Used to view live unstained specimens that are motile
Term
Phase-Contrast Microscope
Definition
Light passes through a condenser modified with an annular diaphragm, light is modified to a hollow cone
Term
Phase Contrast Microscope Use
Definition
-Used to show increased contrast and density within a specimen
Term
Resolution
Definition

allows you to distinguish 2 points that are close to one another

 

Term
Limit Resolution
Definition
Limit resolution=(Wavelengthx6.1*) /numerical aperture
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