Term
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Definition
-May or may not have bulb or taper
-Calibrated to contain a set volume
-total holding volume of less than 1 ml |
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Term
| Factors determining the type of pipette.. |
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Definition
-Viscosity
-accuraccy desired
-volume to be used
-What solution/fluid being pipetted |
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Term
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Definition
| The portion of the electromagnetic spectrum that eyes can see. |
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Term
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Definition
Wavelengths of radiant energy visible or invisible to the human eye.
A wavelength of light is defined as the distance between two peaks, measured in nanometers(nm), as it travels in wavelike patterns. |
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Term
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Definition
Color of light depends on the wavelength that is NOT ABSORBED
What is not absorbed is transmitted. |
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Term
Wavelength of <380
(Ultraviolet) |
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Definition
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Term
380-440
440-500
500-580
580-600
600-620
620-750 |
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Definition
Violet
Blue
Green
Yellow
Orange |
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Term
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Definition
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Term
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Definition
Most Common
Sunlight, incandescent tungsten or tungsten iodide lamp.
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Term
| Lamps most commonly used for Ultraviolet work |
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Definition
| Deuterium- discharge lamp or the Mercury-Arc Lamp |
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Term
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Definition
| Concentration of a substance is directly proportional to the amount of light absorbed or inversly proportional to the logarithm of light transmitted. |
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Term
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Definition
| Concentration of the unknown equals Absorbance of the Unknown(Au) Times the Concentration of Standard(Cs) Divided By The Absorbance of Standard. |
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Term
| Percent Transmittance(%T) |
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Definition
| The Ratio of the radiant energy transmitted(T), divided by the radiant energy incidental(I) on the sample, Expressed as %. |
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Term
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Definition
| Light initially directed toward a sample |
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Term
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Definition
| A measure of the Fraction of initial Radiant Power P0 the passes through a sample, Emerges (p)and reaches a detector. |
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Term
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Definition
The amount of light ABSORBED
Cannot Be Measured Directly, But is Mathmatically Derived From %T as it is directly proportional to concentration. |
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Term
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Definition
A Measure of the amount of Radiant Power absorbed by the sample
-Indirectly Proportional t Transmittance
-Directly Proportional to Concentration |
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Term
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Definition
Methods of measuring concentration by reference to the absorption or transmission of light.
Visual-Interperated by the human eye
Photoelectrical- Interperated by machine |
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Term
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Definition
| Instrument used to measure the light transmitted by a solution in order to determine the concentration of the light absorbing substance in the solution. |
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Term
| Light source of Spectrophotometer |
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Definition
A device which provides EMR in a broad spectrum.
(Source of Light)
Visible and IR- Incandescent Tungsten or Tungsten Iodide Lamp
UV- Deuterium Discharge Lamp or Mercury Arc Lamp |
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Term
| Maintenence of light source for Spectrophotometer |
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Definition
Recalibrate when lamp is changed
-Changes the angle of the light striking the monochromator
-Angle change causes the color of the light striking the cuvet to be changed. |
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Term
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Definition
Device for selecting a range of wavelengths, surrounding the specified wavelength.
Isolation of individual wavelength is important and necessary for function. |
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Term
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Definition
-Colored Glass Filters
-Interference Filters
-Prism
-Diffraction Gratinng (Most commonly used) |
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Term
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Definition
Least Expensive
Usually pass a relatively wide band of radiant energy and have a low transmittance of the selected wavelength.
Not precise.
Simple and useful. |
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Term
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Definition
| Sample cell-a place to hold the sample being analyzed. |
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Term
| Tor F: Quartz Cuvettes must be ised for requiring ultraviolet radiation. |
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Definition
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