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Definition
| the genetic composition of any individual. |
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Definition
| the expression seen in the individual (what it looks like). |
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Definition
| the various forms of one determining factor (of one gene). |
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Definition
| a genotype in which the two alleles in a diploid organism are the same (AA or aa). |
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Definition
| a genotype in which the two alleles in a diploid organism are not the same (Aa or Bb). |
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Definition
| a phenotype exressed whether present as both alleles (homozygous) or as only one of the alleles (heterozygous). |
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Definition
| a phenotype that can only be expressed when present as both alleles (homozygous). |
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Definition
| occurs when a heterozygote shows an INTERMEDIATE phenotype between what would be seen in a homozygous dominant individual and a homozygous recessive individual. |
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Definition
| occurs when BOTH alleles possesed by a heterozygote are expressed in the phenotype. |
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Term
| Identify and define: 3 stages of DNA isolation |
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Definition
1)Homogenization: SLS destructs the cellular and nuclear membranes, separation o chromosomal proteins from the DNA molecules.
2)Denaturation of proteins: Chloroform-isoamyl alcohol denatures proteins and collects them at the interface between the 2 formed layers (because of polar and nonpolar regions).
3)Precipitation of DNA: Ice-cold ethanol precipitates DNA (now thick, stringy, white mass); its cold temp. solidifies long DNA strands, making them easier to be extracted with glass rod. THE END |
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Term
| Appearance of solution (in DNA isolation) after each step in the process (identify steps). |
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Definition
After Homogenization: Foamy
After Filtration: clear liquid
After addition of ethanol: precipitated, or "cow snot"ish. |
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Term
| Be able to describe the roles of NaCl, SLS, 95% Ethanol, and 60 degree incubation in isolation procedure. |
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Definition
NaCl: helps DNA avoid being dissolved
SLS: breaks down cell wall
95% Ethanol: precipitates DNA
60 degree incubation: helps prevent enzymes and nucleauses from breaking DNA down. |
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Term
| Which bacterial strain (A or B) was resistant to the antibiotic Ampicillin and how did we figure that out. |
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Definition
| B was resistant (in Lois's) because the bacteria grew as normal. A was not resistant - barely any of the strain grew. |
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